Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6249 and GFF6250 overlap by 4 nucleotides GFF6250 and GFF6251 are separated by 19 nucleotides GFF6251 and GFF6252 are separated by 10 nucleotides
GFF6249 - Transporter, monovalent cation:proton antiporter-2 (CPA2) family, at 42,942 to 44,213
GFF6249
GFF6250 - Glutamate--cysteine ligase-like protein YbdK, at 44,210 to 45,430
GFF6250
GFF6251 - hypothetical protein, at 45,450 to 46,019
GFF6251
GFF6252 - Phosphonate ABC transporter permease protein PhnE (TC 3.A.1.9.1), at 46,030 to 46,833
GFF6252
Position (kb)
44
45
46 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 43.299 kb on + strand, within GFF6249 at 43.299 kb on + strand, within GFF6249 at 43.299 kb on + strand, within GFF6249 at 43.299 kb on + strand, within GFF6249 at 43.300 kb on - strand, within GFF6249 at 43.300 kb on - strand, within GFF6249 at 43.311 kb on + strand, within GFF6249 at 43.312 kb on - strand, within GFF6249 at 43.312 kb on - strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.477 kb on - strand, within GFF6249 at 43.477 kb on - strand, within GFF6249 at 43.477 kb on - strand, within GFF6249 at 43.477 kb on - strand, within GFF6249 at 43.495 kb on - strand, within GFF6249 at 43.666 kb on + strand, within GFF6249 at 43.666 kb on + strand, within GFF6249 at 43.666 kb on + strand, within GFF6249 at 43.666 kb on + strand, within GFF6249 at 44.390 kb on + strand, within GFF6250 at 44.391 kb on - strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.640 kb on - strand, within GFF6250 at 44.675 kb on + strand, within GFF6250 at 44.675 kb on + strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.778 kb on - strand, within GFF6250 at 44.778 kb on - strand, within GFF6250 at 44.778 kb on - strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.782 kb on - strand, within GFF6250 at 44.928 kb on - strand, within GFF6250 at 44.928 kb on - strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.137 kb on + strand, within GFF6250 at 45.147 kb on - strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.194 kb on + strand, within GFF6250 at 45.194 kb on + strand, within GFF6250 at 45.195 kb on - strand, within GFF6250 at 45.493 kb on + strand at 45.494 kb on - strand at 45.494 kb on - strand at 45.545 kb on - strand, within GFF6251 at 45.775 kb on + strand, within GFF6251 at 45.775 kb on + strand, within GFF6251 at 45.775 kb on + strand, within GFF6251 at 45.776 kb on - strand, within GFF6251 at 45.776 kb on - strand, within GFF6251 at 46.031 kb on + strand at 46.031 kb on + strand at 46.032 kb on - strand at 46.032 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 43,299 + GFF6249 0.28 -1.8 43,299 + GFF6249 0.28 -0.6 43,299 + GFF6249 0.28 -1.6 43,299 + GFF6249 0.28 -1.0 43,300 - GFF6249 0.28 -0.2 43,300 - GFF6249 0.28 -1.8 43,311 + GFF6249 0.29 -1.5 43,312 - GFF6249 0.29 +0.1 43,312 - GFF6249 0.29 -2.3 43,476 + GFF6249 0.42 -2.3 43,476 + GFF6249 0.42 -2.8 43,476 + GFF6249 0.42 -1.8 43,476 + GFF6249 0.42 -1.0 43,476 + GFF6249 0.42 -1.5 43,476 + GFF6249 0.42 -1.8 43,477 - GFF6249 0.42 -1.2 43,477 - GFF6249 0.42 -1.4 43,477 - GFF6249 0.42 -0.2 43,477 - GFF6249 0.42 -0.9 43,495 - GFF6249 0.43 -2.6 43,666 + GFF6249 0.57 -0.6 43,666 + GFF6249 0.57 -1.4 43,666 + GFF6249 0.57 -2.7 43,666 + GFF6249 0.57 -1.3 44,390 + GFF6250 0.15 -1.2 44,391 - GFF6250 0.15 -2.2 44,639 + GFF6250 0.35 -1.1 44,639 + GFF6250 0.35 -2.0 44,639 + GFF6250 0.35 -2.6 44,639 + GFF6250 0.35 -0.9 44,640 - GFF6250 0.35 -2.2 44,675 + GFF6250 0.38 -0.9 44,675 + GFF6250 0.38 -1.1 44,676 - GFF6250 0.38 -1.2 44,676 - GFF6250 0.38 -2.2 44,676 - GFF6250 0.38 -0.1 44,676 - GFF6250 0.38 -3.2 44,778 - GFF6250 0.47 -1.1 44,778 - GFF6250 0.47 -0.8 44,778 - GFF6250 0.47 -1.8 44,781 + GFF6250 0.47 -2.6 44,781 + GFF6250 0.47 -0.8 44,781 + GFF6250 0.47 -1.2 44,781 + GFF6250 0.47 -0.9 44,782 - GFF6250 0.47 -1.3 44,928 - GFF6250 0.59 -1.9 44,928 - GFF6250 0.59 -0.4 45,074 + GFF6250 0.71 -1.6 45,074 + GFF6250 0.71 -0.6 45,074 + GFF6250 0.71 -1.6 45,074 + GFF6250 0.71 -1.9 45,075 - GFF6250 0.71 -1.8 45,075 - GFF6250 0.71 -2.0 45,075 - GFF6250 0.71 -1.9 45,075 - GFF6250 0.71 -1.2 45,137 + GFF6250 0.76 -1.2 45,147 - GFF6250 0.77 -2.7 45,152 + GFF6250 0.77 -0.7 45,152 + GFF6250 0.77 -2.1 45,152 + GFF6250 0.77 -2.1 45,152 + GFF6250 0.77 -1.0 45,152 + GFF6250 0.77 -2.1 45,153 - GFF6250 0.77 +1.8 45,153 - GFF6250 0.77 -0.6 45,153 - GFF6250 0.77 -1.9 45,153 - GFF6250 0.77 -1.1 45,153 - GFF6250 0.77 -2.2 45,194 + GFF6250 0.81 -1.5 45,194 + GFF6250 0.81 -1.8 45,195 - GFF6250 0.81 -0.2 45,493 + -0.4 45,494 - +0.1 45,494 - -0.0 45,545 - GFF6251 0.17 -0.2 45,775 + GFF6251 0.57 +0.8 45,775 + GFF6251 0.57 -0.5 45,775 + GFF6251 0.57 +0.5 45,776 - GFF6251 0.57 -0.7 45,776 - GFF6251 0.57 +0.8 46,031 + -1.6 46,031 + +0.8 46,032 - -0.5 46,032 - -2.2
Or see this region's nucleotide sequence