Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6208 and GFF6209 are separated by 192 nucleotides GFF6209 and GFF6210 overlap by 4 nucleotides GFF6210 and GFF6211 are separated by 25 nucleotides GFF6211 and GFF6212 are separated by 169 nucleotides
GFF6208 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 115,313 to 116,806
GFF6208
GFF6209 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 116,999 to 117,742
GFF6209
GFF6210 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 117,739 to 118,527
GFF6210
GFF6211 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 118,553 to 119,317
GFF6211
GFF6212 - Transcriptional regulator, AsnC family, at 119,487 to 119,909
GFF6212
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 116.932 kb on + strand at 116.932 kb on + strand at 116.933 kb on - strand at 116.933 kb on - strand at 116.933 kb on - strand at 116.933 kb on - strand at 116.934 kb on + strand at 116.934 kb on + strand at 116.935 kb on - strand at 116.940 kb on + strand at 116.941 kb on - strand at 116.941 kb on - strand at 117.241 kb on + strand, within GFF6209 at 117.241 kb on + strand, within GFF6209 at 117.242 kb on - strand, within GFF6209 at 117.242 kb on - strand, within GFF6209 at 117.368 kb on + strand, within GFF6209 at 117.818 kb on + strand, within GFF6210 at 117.818 kb on + strand, within GFF6210 at 117.819 kb on - strand, within GFF6210 at 117.819 kb on - strand, within GFF6210 at 117.819 kb on - strand, within GFF6210 at 117.819 kb on - strand, within GFF6210 at 117.819 kb on - strand, within GFF6210 at 117.883 kb on + strand, within GFF6210 at 117.883 kb on + strand, within GFF6210 at 117.883 kb on + strand, within GFF6210 at 117.883 kb on + strand, within GFF6210 at 117.883 kb on + strand, within GFF6210 at 117.884 kb on - strand, within GFF6210 at 117.884 kb on - strand, within GFF6210 at 118.013 kb on + strand, within GFF6210 at 118.014 kb on - strand, within GFF6210 at 118.014 kb on - strand, within GFF6210 at 118.014 kb on - strand, within GFF6210 at 118.100 kb on + strand, within GFF6210 at 118.101 kb on - strand, within GFF6210 at 118.101 kb on - strand, within GFF6210 at 118.101 kb on - strand, within GFF6210 at 118.101 kb on - strand, within GFF6210 at 118.236 kb on + strand, within GFF6210 at 118.236 kb on + strand, within GFF6210 at 118.236 kb on + strand, within GFF6210 at 118.236 kb on + strand, within GFF6210 at 118.238 kb on + strand, within GFF6210 at 118.238 kb on + strand, within GFF6210 at 118.238 kb on + strand, within GFF6210 at 118.238 kb on + strand, within GFF6210 at 118.238 kb on + strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.564 kb on + strand at 118.564 kb on + strand at 118.564 kb on + strand at 118.564 kb on + strand at 118.565 kb on - strand at 118.565 kb on - strand at 118.565 kb on - strand at 118.565 kb on - strand at 118.565 kb on - strand at 118.581 kb on + strand at 118.581 kb on + strand at 118.581 kb on + strand at 118.581 kb on + strand at 118.582 kb on - strand at 118.582 kb on - strand at 118.582 kb on - strand at 118.602 kb on + strand at 118.602 kb on + strand at 118.602 kb on + strand at 118.602 kb on + strand at 118.603 kb on - strand at 118.603 kb on - strand at 118.603 kb on - strand at 118.603 kb on - strand at 118.603 kb on - strand at 118.603 kb on - strand at 118.641 kb on + strand, within GFF6211 at 118.784 kb on + strand, within GFF6211 at 118.784 kb on + strand, within GFF6211 at 118.785 kb on - strand, within GFF6211 at 118.785 kb on - strand, within GFF6211 at 118.785 kb on - strand, within GFF6211 at 118.785 kb on - strand, within GFF6211 at 118.785 kb on - strand, within GFF6211 at 118.878 kb on + strand, within GFF6211 at 118.878 kb on + strand, within GFF6211 at 118.878 kb on + strand, within GFF6211 at 118.879 kb on - strand, within GFF6211 at 118.879 kb on - strand, within GFF6211 at 118.879 kb on - strand, within GFF6211 at 118.879 kb on - strand, within GFF6211 at 118.879 kb on - strand, within GFF6211 at 118.975 kb on - strand, within GFF6211 at 118.975 kb on - strand, within GFF6211 at 118.975 kb on - strand, within GFF6211 at 119.178 kb on + strand, within GFF6211 at 119.178 kb on + strand, within GFF6211 at 119.178 kb on + strand, within GFF6211 at 119.178 kb on + strand, within GFF6211 at 119.178 kb on + strand, within GFF6211 at 119.178 kb on + strand, within GFF6211 at 119.179 kb on - strand, within GFF6211 at 119.179 kb on - strand, within GFF6211 at 119.267 kb on + strand at 119.267 kb on + strand at 119.267 kb on + strand at 119.267 kb on + strand at 119.267 kb on + strand at 119.268 kb on - strand at 119.268 kb on - strand at 119.268 kb on - strand at 119.268 kb on - strand at 119.268 kb on - strand at 119.268 kb on - strand at 119.268 kb on - strand at 119.268 kb on - strand at 119.268 kb on - strand at 119.268 kb on - strand at 119.526 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 116,932 + -0.8 116,932 + -0.1 116,933 - -0.8 116,933 - -0.8 116,933 - -0.1 116,933 - +0.0 116,934 + -0.2 116,934 + -0.3 116,935 - -0.5 116,940 + -1.9 116,941 - +0.8 116,941 - +0.2 117,241 + GFF6209 0.33 -1.6 117,241 + GFF6209 0.33 +0.4 117,242 - GFF6209 0.33 -0.6 117,242 - GFF6209 0.33 +0.1 117,368 + GFF6209 0.50 -0.5 117,818 + GFF6210 0.10 +0.0 117,818 + GFF6210 0.10 +0.9 117,819 - GFF6210 0.10 -1.4 117,819 - GFF6210 0.10 +0.0 117,819 - GFF6210 0.10 -1.3 117,819 - GFF6210 0.10 -0.3 117,819 - GFF6210 0.10 -0.1 117,883 + GFF6210 0.18 -0.3 117,883 + GFF6210 0.18 +1.4 117,883 + GFF6210 0.18 -0.3 117,883 + GFF6210 0.18 -0.6 117,883 + GFF6210 0.18 -0.3 117,884 - GFF6210 0.18 +0.1 117,884 - GFF6210 0.18 +0.6 118,013 + GFF6210 0.35 +0.2 118,014 - GFF6210 0.35 -0.2 118,014 - GFF6210 0.35 -0.3 118,014 - GFF6210 0.35 -0.4 118,100 + GFF6210 0.46 -0.1 118,101 - GFF6210 0.46 -0.0 118,101 - GFF6210 0.46 +1.7 118,101 - GFF6210 0.46 +0.4 118,101 - GFF6210 0.46 +0.9 118,236 + GFF6210 0.63 +0.3 118,236 + GFF6210 0.63 -1.1 118,236 + GFF6210 0.63 +0.3 118,236 + GFF6210 0.63 +1.5 118,238 + GFF6210 0.63 +1.2 118,238 + GFF6210 0.63 +0.1 118,238 + GFF6210 0.63 -1.3 118,238 + GFF6210 0.63 +1.7 118,238 + GFF6210 0.63 +0.1 118,239 - GFF6210 0.63 +0.7 118,239 - GFF6210 0.63 +0.3 118,239 - GFF6210 0.63 -1.0 118,239 - GFF6210 0.63 +0.4 118,239 - GFF6210 0.63 +0.7 118,239 - GFF6210 0.63 -1.3 118,239 - GFF6210 0.63 +0.0 118,239 - GFF6210 0.63 +0.4 118,564 + +0.1 118,564 + +0.0 118,564 + -0.5 118,564 + +0.1 118,565 - -1.1 118,565 - -0.5 118,565 - -0.5 118,565 - +0.0 118,565 - -0.5 118,581 + -0.7 118,581 + -1.9 118,581 + +0.1 118,581 + +0.2 118,582 - +0.0 118,582 - -1.1 118,582 - +0.6 118,602 + +0.9 118,602 + -1.0 118,602 + +0.7 118,602 + -0.6 118,603 - +1.7 118,603 - +0.1 118,603 - -0.6 118,603 - -0.5 118,603 - +0.7 118,603 - +0.8 118,641 + GFF6211 0.12 +0.1 118,784 + GFF6211 0.30 -0.5 118,784 + GFF6211 0.30 +2.0 118,785 - GFF6211 0.30 +0.1 118,785 - GFF6211 0.30 +0.4 118,785 - GFF6211 0.30 -0.9 118,785 - GFF6211 0.30 +0.9 118,785 - GFF6211 0.30 +0.1 118,878 + GFF6211 0.42 +2.0 118,878 + GFF6211 0.42 -0.5 118,878 + GFF6211 0.42 +0.1 118,879 - GFF6211 0.43 +0.3 118,879 - GFF6211 0.43 +0.3 118,879 - GFF6211 0.43 +0.8 118,879 - GFF6211 0.43 -2.1 118,879 - GFF6211 0.43 -0.6 118,975 - GFF6211 0.55 +0.5 118,975 - GFF6211 0.55 +1.3 118,975 - GFF6211 0.55 -0.3 119,178 + GFF6211 0.82 -0.2 119,178 + GFF6211 0.82 +1.3 119,178 + GFF6211 0.82 -0.2 119,178 + GFF6211 0.82 +1.2 119,178 + GFF6211 0.82 +1.2 119,178 + GFF6211 0.82 +0.4 119,179 - GFF6211 0.82 -0.4 119,179 - GFF6211 0.82 +0.2 119,267 + -0.8 119,267 + -0.1 119,267 + -0.1 119,267 + -0.8 119,267 + +0.8 119,268 - +1.0 119,268 - -0.4 119,268 - +0.3 119,268 - -0.1 119,268 - -2.6 119,268 - +0.6 119,268 - +1.0 119,268 - -0.2 119,268 - -0.4 119,268 - -0.2 119,526 - -0.0
Or see this region's nucleotide sequence