Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6180 and GFF6181 are separated by 1 nucleotides GFF6181 and GFF6182 are separated by 19 nucleotides GFF6182 and GFF6183 are separated by 29 nucleotides
GFF6180 - 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) / Cytidylate kinase (EC 2.7.4.25), at 84,510 to 86,522
GFF6180
GFF6181 - Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43), at 86,524 to 87,414
GFF6181
GFF6182 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 87,434 to 88,558
GFF6182
GFF6183 - Phosphoserine aminotransferase (EC 2.6.1.52), at 88,588 to 89,718
GFF6183
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 86.826 kb on - strand, within GFF6181 at 87.468 kb on + strand at 87.469 kb on - strand at 87.469 kb on - strand at 87.546 kb on + strand at 87.546 kb on + strand at 87.546 kb on + strand at 87.546 kb on + strand at 87.547 kb on - strand, within GFF6182 at 87.547 kb on - strand, within GFF6182 at 87.547 kb on - strand, within GFF6182 at 87.547 kb on - strand, within GFF6182 at 87.547 kb on - strand, within GFF6182 at 87.547 kb on - strand, within GFF6182 at 87.547 kb on - strand, within GFF6182 at 87.552 kb on + strand, within GFF6182 at 87.552 kb on + strand, within GFF6182 at 87.553 kb on - strand, within GFF6182 at 87.553 kb on - strand, within GFF6182 at 87.553 kb on - strand, within GFF6182 at 87.553 kb on - strand, within GFF6182 at 87.553 kb on - strand, within GFF6182 at 87.553 kb on - strand, within GFF6182 at 87.556 kb on - strand, within GFF6182 at 87.556 kb on - strand, within GFF6182 at 87.556 kb on - strand, within GFF6182 at 87.782 kb on + strand, within GFF6182 at 87.782 kb on + strand, within GFF6182 at 88.031 kb on + strand, within GFF6182 at 88.031 kb on + strand, within GFF6182 at 88.031 kb on + strand, within GFF6182 at 88.589 kb on + strand at 88.589 kb on + strand at 88.590 kb on - strand at 88.590 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 86,826 - GFF6181 0.34 +0.5 87,468 + -2.3 87,469 - -1.9 87,469 - -2.3 87,546 + -1.1 87,546 + -1.3 87,546 + -0.9 87,546 + -3.4 87,547 - GFF6182 0.10 -0.6 87,547 - GFF6182 0.10 -0.6 87,547 - GFF6182 0.10 -0.3 87,547 - GFF6182 0.10 +1.1 87,547 - GFF6182 0.10 -0.3 87,547 - GFF6182 0.10 -1.6 87,547 - GFF6182 0.10 -1.8 87,552 + GFF6182 0.10 -1.5 87,552 + GFF6182 0.10 -0.3 87,553 - GFF6182 0.11 -1.1 87,553 - GFF6182 0.11 -1.5 87,553 - GFF6182 0.11 -1.1 87,553 - GFF6182 0.11 +0.1 87,553 - GFF6182 0.11 +0.1 87,553 - GFF6182 0.11 +0.1 87,556 - GFF6182 0.11 -2.7 87,556 - GFF6182 0.11 -0.4 87,556 - GFF6182 0.11 -3.2 87,782 + GFF6182 0.31 -1.9 87,782 + GFF6182 0.31 -2.7 88,031 + GFF6182 0.53 -1.1 88,031 + GFF6182 0.53 -1.8 88,031 + GFF6182 0.53 -0.1 88,589 + -0.3 88,589 + -2.6 88,590 - +1.2 88,590 - +0.3
Or see this region's nucleotide sequence