Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6104 and GFF6105 are separated by 14 nucleotides GFF6105 and GFF6106 are separated by 102 nucleotides GFF6106 and GFF6107 are separated by 120 nucleotides
GFF6104 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 3,983 to 4,873
GFF6104
GFF6105 - Aspartate racemase (EC 5.1.1.13), at 4,888 to 5,637
GFF6105
GFF6106 - Dienelactone hydrolase family protein, at 5,740 to 6,636
GFF6106
GFF6107 - Zinc transporter, ZIP family, at 6,757 to 7,647
GFF6107
Position (kb) 5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5.311 kb on + strand, within GFF6105 at 5.311 kb on + strand, within GFF6105 at 5.311 kb on + strand, within GFF6105 at 5.312 kb on - strand, within GFF6105 at 5.349 kb on - strand, within GFF6105 at 5.614 kb on + strand at 5.649 kb on + strand at 5.649 kb on + strand at 5.650 kb on - strand at 5.650 kb on - strand at 5.650 kb on - strand at 5.650 kb on - strand at 5.673 kb on + strand at 5.674 kb on - strand at 5.674 kb on - strand at 5.848 kb on + strand, within GFF6106 at 5.848 kb on + strand, within GFF6106 at 5.848 kb on + strand, within GFF6106 at 5.929 kb on + strand, within GFF6106 at 5.929 kb on + strand, within GFF6106 at 5.929 kb on + strand, within GFF6106 at 5.929 kb on + strand, within GFF6106 at 5.929 kb on + strand, within GFF6106 at 5.930 kb on - strand, within GFF6106 at 5.930 kb on - strand, within GFF6106 at 5.930 kb on - strand, within GFF6106 at 5.930 kb on - strand, within GFF6106 at 5.959 kb on + strand, within GFF6106 at 5.959 kb on + strand, within GFF6106 at 5.960 kb on - strand, within GFF6106 at 6.034 kb on + strand, within GFF6106 at 6.034 kb on + strand, within GFF6106 at 6.034 kb on + strand, within GFF6106 at 6.035 kb on - strand, within GFF6106 at 6.035 kb on - strand, within GFF6106 at 6.125 kb on - strand, within GFF6106 at 6.211 kb on + strand, within GFF6106 at 6.211 kb on + strand, within GFF6106 at 6.212 kb on - strand, within GFF6106 at 6.212 kb on - strand, within GFF6106 at 6.289 kb on + strand, within GFF6106 at 6.459 kb on + strand, within GFF6106 at 6.459 kb on + strand, within GFF6106 at 6.520 kb on + strand, within GFF6106 at 6.520 kb on + strand, within GFF6106 at 6.521 kb on - strand, within GFF6106 at 6.565 kb on + strand at 6.566 kb on - strand at 6.566 kb on - strand at 6.651 kb on - strand at 6.651 kb on - strand at 6.698 kb on + strand at 6.699 kb on - strand at 6.768 kb on - strand at 7.450 kb on + strand, within GFF6107 at 7.450 kb on + strand, within GFF6107 at 7.450 kb on + strand, within GFF6107 at 7.451 kb on - strand, within GFF6107
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 5,311 + GFF6105 0.56 -1.6 5,311 + GFF6105 0.56 +0.4 5,311 + GFF6105 0.56 -0.2 5,312 - GFF6105 0.57 -1.1 5,349 - GFF6105 0.61 +1.1 5,614 + -0.1 5,649 + -0.3 5,649 + +0.4 5,650 - -0.3 5,650 - -0.2 5,650 - -0.6 5,650 - +0.4 5,673 + -0.5 5,674 - +0.7 5,674 - +1.0 5,848 + GFF6106 0.12 -1.3 5,848 + GFF6106 0.12 -0.9 5,848 + GFF6106 0.12 -0.6 5,929 + GFF6106 0.21 -0.4 5,929 + GFF6106 0.21 -0.3 5,929 + GFF6106 0.21 +0.4 5,929 + GFF6106 0.21 +0.2 5,929 + GFF6106 0.21 +0.5 5,930 - GFF6106 0.21 -0.5 5,930 - GFF6106 0.21 +0.5 5,930 - GFF6106 0.21 -1.0 5,930 - GFF6106 0.21 -0.6 5,959 + GFF6106 0.24 -2.9 5,959 + GFF6106 0.24 -0.6 5,960 - GFF6106 0.25 -0.3 6,034 + GFF6106 0.33 +0.1 6,034 + GFF6106 0.33 -0.3 6,034 + GFF6106 0.33 +0.3 6,035 - GFF6106 0.33 +0.2 6,035 - GFF6106 0.33 -0.2 6,125 - GFF6106 0.43 -0.8 6,211 + GFF6106 0.53 -0.6 6,211 + GFF6106 0.53 -0.6 6,212 - GFF6106 0.53 +0.2 6,212 - GFF6106 0.53 +1.0 6,289 + GFF6106 0.61 +0.3 6,459 + GFF6106 0.80 +0.1 6,459 + GFF6106 0.80 -0.4 6,520 + GFF6106 0.87 -0.2 6,520 + GFF6106 0.87 -1.5 6,521 - GFF6106 0.87 -0.0 6,565 + +0.3 6,566 - -1.6 6,566 - +0.9 6,651 - -0.9 6,651 - +0.2 6,698 + -0.2 6,699 - -1.4 6,768 - +0.5 7,450 + GFF6107 0.78 +0.8 7,450 + GFF6107 0.78 -0.2 7,450 + GFF6107 0.78 -1.3 7,451 - GFF6107 0.78 -1.4
Or see this region's nucleotide sequence