Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5945 and GFF5946 are separated by 60 nucleotides
GFF5945 - Membrane-bound lytic murein transglycosylase A, at 166,195 to 167,796
GFF5945
GFF5946 - T6SS Serine/threonine protein kinase (EC 2.7.11.1) PpkA, at 167,857 to 169,785
GFF5946
Position (kb)
167
168
169
170 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 166.892 kb on + strand, within GFF5945 at 167.036 kb on + strand, within GFF5945 at 167.037 kb on - strand, within GFF5945 at 167.037 kb on - strand, within GFF5945 at 167.039 kb on + strand, within GFF5945 at 167.039 kb on + strand, within GFF5945 at 167.265 kb on - strand, within GFF5945 at 167.265 kb on - strand, within GFF5945 at 167.265 kb on - strand, within GFF5945 at 167.325 kb on - strand, within GFF5945 at 167.372 kb on + strand, within GFF5945 at 167.372 kb on + strand, within GFF5945 at 167.570 kb on + strand, within GFF5945 at 167.570 kb on + strand, within GFF5945 at 167.571 kb on - strand, within GFF5945 at 167.571 kb on - strand, within GFF5945 at 167.595 kb on + strand, within GFF5945 at 167.595 kb on + strand, within GFF5945 at 167.596 kb on - strand, within GFF5945 at 167.858 kb on + strand at 167.858 kb on + strand at 167.859 kb on - strand at 167.859 kb on - strand at 167.859 kb on - strand at 167.891 kb on + strand at 167.892 kb on - strand at 167.892 kb on - strand at 168.162 kb on - strand, within GFF5946 at 168.162 kb on - strand, within GFF5946 at 168.162 kb on - strand, within GFF5946 at 168.162 kb on - strand, within GFF5946 at 168.162 kb on - strand, within GFF5946 at 168.224 kb on + strand, within GFF5946 at 168.224 kb on + strand, within GFF5946 at 168.225 kb on - strand, within GFF5946 at 168.225 kb on - strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.915 kb on - strand, within GFF5946 at 168.915 kb on - strand, within GFF5946 at 168.915 kb on - strand, within GFF5946 at 168.915 kb on - strand, within GFF5946 at 168.915 kb on - strand, within GFF5946 at 168.996 kb on - strand, within GFF5946 at 169.040 kb on + strand, within GFF5946 at 169.040 kb on + strand, within GFF5946 at 169.193 kb on + strand, within GFF5946 at 169.194 kb on - strand, within GFF5946 at 169.194 kb on - strand, within GFF5946 at 169.316 kb on + strand, within GFF5946 at 169.316 kb on + strand, within GFF5946 at 169.317 kb on - strand, within GFF5946 at 169.317 kb on - strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.365 kb on - strand, within GFF5946 at 169.517 kb on + strand, within GFF5946 at 169.517 kb on + strand, within GFF5946 at 169.518 kb on - strand, within GFF5946 at 169.518 kb on - strand, within GFF5946 at 169.518 kb on - strand, within GFF5946 at 169.565 kb on + strand, within GFF5946 at 169.566 kb on - strand, within GFF5946 at 169.591 kb on + strand, within GFF5946 at 169.631 kb on - strand at 169.753 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 166,892 + GFF5945 0.44 +0.2 167,036 + GFF5945 0.52 -1.3 167,037 - GFF5945 0.53 +0.6 167,037 - GFF5945 0.53 -0.8 167,039 + GFF5945 0.53 -0.2 167,039 + GFF5945 0.53 -0.1 167,265 - GFF5945 0.67 +0.7 167,265 - GFF5945 0.67 -1.2 167,265 - GFF5945 0.67 +1.2 167,325 - GFF5945 0.71 -0.5 167,372 + GFF5945 0.73 +0.0 167,372 + GFF5945 0.73 +0.2 167,570 + GFF5945 0.86 -0.1 167,570 + GFF5945 0.86 +1.2 167,571 - GFF5945 0.86 -0.7 167,571 - GFF5945 0.86 +1.2 167,595 + GFF5945 0.87 -0.1 167,595 + GFF5945 0.87 -2.3 167,596 - GFF5945 0.87 -0.1 167,858 + -0.8 167,858 + -0.6 167,859 - +1.8 167,859 - +1.6 167,859 - +0.5 167,891 + -1.0 167,892 - +0.3 167,892 - -0.3 168,162 - GFF5946 0.16 +0.2 168,162 - GFF5946 0.16 -0.5 168,162 - GFF5946 0.16 +0.2 168,162 - GFF5946 0.16 +0.8 168,162 - GFF5946 0.16 +0.4 168,224 + GFF5946 0.19 -0.2 168,224 + GFF5946 0.19 -1.2 168,225 - GFF5946 0.19 -0.6 168,225 - GFF5946 0.19 -1.0 168,914 + GFF5946 0.55 -0.2 168,914 + GFF5946 0.55 -0.0 168,914 + GFF5946 0.55 +0.7 168,914 + GFF5946 0.55 -0.8 168,914 + GFF5946 0.55 -0.5 168,914 + GFF5946 0.55 -0.1 168,914 + GFF5946 0.55 -0.1 168,914 + GFF5946 0.55 -0.6 168,914 + GFF5946 0.55 -0.5 168,915 - GFF5946 0.55 -1.2 168,915 - GFF5946 0.55 -0.5 168,915 - GFF5946 0.55 -0.7 168,915 - GFF5946 0.55 -0.6 168,915 - GFF5946 0.55 +0.1 168,996 - GFF5946 0.59 -0.7 169,040 + GFF5946 0.61 -1.4 169,040 + GFF5946 0.61 -1.0 169,193 + GFF5946 0.69 -0.3 169,194 - GFF5946 0.69 -1.1 169,194 - GFF5946 0.69 +0.1 169,316 + GFF5946 0.76 +0.3 169,316 + GFF5946 0.76 -1.5 169,317 - GFF5946 0.76 +0.0 169,317 - GFF5946 0.76 +0.7 169,334 + GFF5946 0.77 -1.0 169,334 + GFF5946 0.77 -1.9 169,334 + GFF5946 0.77 +1.1 169,334 + GFF5946 0.77 -0.1 169,334 + GFF5946 0.77 -1.2 169,334 + GFF5946 0.77 +0.9 169,334 + GFF5946 0.77 -1.0 169,334 + GFF5946 0.77 -0.0 169,335 - GFF5946 0.77 -1.0 169,335 - GFF5946 0.77 -0.8 169,335 - GFF5946 0.77 +0.4 169,335 - GFF5946 0.77 -0.2 169,335 - GFF5946 0.77 -0.5 169,335 - GFF5946 0.77 +0.1 169,365 - GFF5946 0.78 -0.4 169,517 + GFF5946 0.86 +0.5 169,517 + GFF5946 0.86 -0.3 169,518 - GFF5946 0.86 +2.2 169,518 - GFF5946 0.86 +0.2 169,518 - GFF5946 0.86 -2.1 169,565 + GFF5946 0.89 -0.4 169,566 - GFF5946 0.89 +0.8 169,591 + GFF5946 0.90 -0.2 169,631 - -0.8 169,753 + +0.1
Or see this region's nucleotide sequence