Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5770 and GFF5771 overlap by 4 nucleotides GFF5771 and GFF5772 overlap by 4 nucleotides
GFF5770 - Flagellar biosynthesis protein FlhB, at 141,916 to 143,070
GFF5770
GFF5771 - Flagellar biosynthesis protein FlhA, at 143,067 to 145,166
GFF5771
GFF5772 - Flagellar biosynthesis protein FlhF, at 145,163 to 147,700
GFF5772
Position (kb) 143
144
145
146 Strain fitness (log2 ratio) -1
0
1
2
3 at 142.456 kb on + strand, within GFF5770 at 142.456 kb on + strand, within GFF5770 at 142.457 kb on - strand, within GFF5770 at 142.457 kb on - strand, within GFF5770 at 142.561 kb on + strand, within GFF5770 at 142.562 kb on - strand, within GFF5770 at 142.562 kb on - strand, within GFF5770 at 142.750 kb on + strand, within GFF5770 at 142.750 kb on + strand, within GFF5770 at 142.751 kb on - strand, within GFF5770 at 142.882 kb on + strand, within GFF5770 at 142.958 kb on - strand at 143.002 kb on - strand at 143.038 kb on + strand at 143.038 kb on + strand at 143.039 kb on - strand at 143.039 kb on - strand at 143.039 kb on - strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.653 kb on - strand, within GFF5771 at 143.793 kb on + strand, within GFF5771 at 143.793 kb on + strand, within GFF5771 at 143.793 kb on + strand, within GFF5771 at 143.794 kb on - strand, within GFF5771 at 144.337 kb on - strand, within GFF5771 at 144.337 kb on - strand, within GFF5771 at 144.513 kb on + strand, within GFF5771 at 144.514 kb on - strand, within GFF5771 at 144.514 kb on - strand, within GFF5771 at 144.520 kb on - strand, within GFF5771 at 144.571 kb on + strand, within GFF5771 at 144.571 kb on + strand, within GFF5771 at 145.734 kb on - strand, within GFF5772 at 145.751 kb on + strand, within GFF5772 at 145.751 kb on + strand, within GFF5772 at 145.752 kb on - strand, within GFF5772 at 145.752 kb on - strand, within GFF5772 at 145.752 kb on - strand, within GFF5772 at 145.752 kb on - strand, within GFF5772 at 145.826 kb on + strand, within GFF5772 at 145.940 kb on + strand, within GFF5772 at 145.941 kb on - strand, within GFF5772 at 145.956 kb on - strand, within GFF5772 at 145.973 kb on + strand, within GFF5772 at 145.973 kb on + strand, within GFF5772 at 145.973 kb on + strand, within GFF5772 at 145.974 kb on - strand, within GFF5772 at 145.974 kb on - strand, within GFF5772 at 145.992 kb on + strand, within GFF5772 at 145.992 kb on + strand, within GFF5772 at 146.000 kb on + strand, within GFF5772 at 146.001 kb on - strand, within GFF5772
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 142,456 + GFF5770 0.47 +0.5 142,456 + GFF5770 0.47 -1.0 142,457 - GFF5770 0.47 -0.2 142,457 - GFF5770 0.47 -0.3 142,561 + GFF5770 0.56 -0.5 142,562 - GFF5770 0.56 -0.7 142,562 - GFF5770 0.56 +0.7 142,750 + GFF5770 0.72 -0.3 142,750 + GFF5770 0.72 -0.1 142,751 - GFF5770 0.72 -0.4 142,882 + GFF5770 0.84 -0.7 142,958 - -0.8 143,002 - -0.8 143,038 + -0.2 143,038 + +2.9 143,039 - -0.3 143,039 - +1.5 143,039 - -0.3 143,259 + -0.8 143,259 + -0.4 143,259 + -0.5 143,259 + +0.6 143,259 + -0.1 143,260 - -1.1 143,260 - +0.0 143,260 - +0.3 143,260 - -0.5 143,260 - +0.4 143,260 - -1.5 143,653 - GFF5771 0.28 -0.5 143,793 + GFF5771 0.35 -1.2 143,793 + GFF5771 0.35 +0.6 143,793 + GFF5771 0.35 +1.8 143,794 - GFF5771 0.35 +2.8 144,337 - GFF5771 0.60 -0.3 144,337 - GFF5771 0.60 +0.4 144,513 + GFF5771 0.69 +0.9 144,514 - GFF5771 0.69 +2.9 144,514 - GFF5771 0.69 +0.3 144,520 - GFF5771 0.69 +0.8 144,571 + GFF5771 0.72 -0.6 144,571 + GFF5771 0.72 -0.8 145,734 - GFF5772 0.22 +0.2 145,751 + GFF5772 0.23 -0.4 145,751 + GFF5772 0.23 +0.7 145,752 - GFF5772 0.23 +0.5 145,752 - GFF5772 0.23 -1.2 145,752 - GFF5772 0.23 +0.6 145,752 - GFF5772 0.23 -0.8 145,826 + GFF5772 0.26 -0.2 145,940 + GFF5772 0.31 +0.5 145,941 - GFF5772 0.31 +0.2 145,956 - GFF5772 0.31 +0.0 145,973 + GFF5772 0.32 +0.3 145,973 + GFF5772 0.32 -1.0 145,973 + GFF5772 0.32 +0.2 145,974 - GFF5772 0.32 -0.5 145,974 - GFF5772 0.32 +0.1 145,992 + GFF5772 0.33 -0.3 145,992 + GFF5772 0.33 +0.1 146,000 + GFF5772 0.33 +0.2 146,001 - GFF5772 0.33 -0.4
Or see this region's nucleotide sequence