Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF55 and GFF56 overlap by 8 nucleotides GFF56 and GFF57 are separated by 67 nucleotides GFF57 and GFF58 are separated by 93 nucleotides
GFF55 - hypothetical protein, at 69,853 to 70,671
GFF55
GFF56 - hypothetical protein, at 70,664 to 71,593
GFF56
GFF57 - Putative polysaccharide export protein YccZ precursor, at 71,661 to 72,872
GFF57
GFF58 - Tyrosine-protein kinase (EC 2.7.10.2), at 72,966 to 75,230
GFF58
Position (kb) 71
72
73 Strain fitness (log2 ratio) -2
-1
0
1
2 at 71.327 kb on + strand, within GFF56 at 71.327 kb on + strand, within GFF56 at 71.328 kb on - strand, within GFF56 at 71.328 kb on - strand, within GFF56 at 71.328 kb on - strand, within GFF56 at 71.412 kb on - strand, within GFF56 at 71.616 kb on + strand at 71.617 kb on - strand at 71.983 kb on - strand, within GFF57 at 71.983 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.022 kb on - strand, within GFF57 at 72.141 kb on + strand, within GFF57 at 72.274 kb on - strand, within GFF57 at 72.525 kb on + strand, within GFF57 at 72.526 kb on - strand, within GFF57 at 72.526 kb on - strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.651 kb on + strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.652 kb on - strand, within GFF57 at 72.771 kb on + strand at 72.771 kb on + strand at 72.771 kb on + strand at 72.771 kb on + strand at 72.772 kb on - strand at 72.772 kb on - strand at 73.084 kb on - strand at 73.278 kb on + strand, within GFF58 at 73.278 kb on + strand, within GFF58 at 73.279 kb on - strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.299 kb on + strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.300 kb on - strand, within GFF58 at 73.378 kb on - strand, within GFF58 at 73.476 kb on + strand, within GFF58 at 73.476 kb on + strand, within GFF58 at 73.477 kb on - strand, within GFF58 at 73.477 kb on - strand, within GFF58 at 73.620 kb on + strand, within GFF58 at 73.621 kb on - strand, within GFF58 at 73.621 kb on - strand, within GFF58 at 73.764 kb on + strand, within GFF58 at 73.764 kb on + strand, within GFF58 at 73.764 kb on + strand, within GFF58 at 73.765 kb on - strand, within GFF58 at 73.765 kb on - strand, within GFF58 at 73.765 kb on - strand, within GFF58
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 71,327 + GFF56 0.71 +0.8 71,327 + GFF56 0.71 -1.0 71,328 - GFF56 0.71 +0.4 71,328 - GFF56 0.71 +0.3 71,328 - GFF56 0.71 -2.1 71,412 - GFF56 0.80 -0.6 71,616 + -0.2 71,617 - +0.6 71,983 - GFF57 0.27 -0.3 71,983 - GFF57 0.27 +0.4 72,022 - GFF57 0.30 -1.2 72,022 - GFF57 0.30 -0.4 72,022 - GFF57 0.30 -1.8 72,141 + GFF57 0.40 -1.4 72,274 - GFF57 0.51 -0.0 72,525 + GFF57 0.71 -0.1 72,526 - GFF57 0.71 +0.1 72,526 - GFF57 0.71 +1.1 72,651 + GFF57 0.82 -0.0 72,651 + GFF57 0.82 +1.2 72,651 + GFF57 0.82 +0.1 72,651 + GFF57 0.82 +1.3 72,652 - GFF57 0.82 +0.0 72,652 - GFF57 0.82 +0.0 72,652 - GFF57 0.82 +0.4 72,652 - GFF57 0.82 +0.2 72,652 - GFF57 0.82 -0.2 72,771 + +1.0 72,771 + -0.6 72,771 + -0.2 72,771 + +0.6 72,772 - -0.1 72,772 - -1.8 73,084 - -0.8 73,278 + GFF58 0.14 -0.6 73,278 + GFF58 0.14 +0.6 73,279 - GFF58 0.14 -0.8 73,299 + GFF58 0.15 -1.0 73,299 + GFF58 0.15 -0.2 73,299 + GFF58 0.15 +0.0 73,299 + GFF58 0.15 -1.1 73,299 + GFF58 0.15 -0.3 73,299 + GFF58 0.15 +0.3 73,300 - GFF58 0.15 -1.0 73,300 - GFF58 0.15 +1.6 73,300 - GFF58 0.15 -0.5 73,300 - GFF58 0.15 +0.6 73,300 - GFF58 0.15 +1.8 73,378 - GFF58 0.18 -1.1 73,476 + GFF58 0.23 -0.1 73,476 + GFF58 0.23 -0.1 73,477 - GFF58 0.23 +0.7 73,477 - GFF58 0.23 -1.5 73,620 + GFF58 0.29 +0.0 73,621 - GFF58 0.29 +0.0 73,621 - GFF58 0.29 -0.6 73,764 + GFF58 0.35 -0.4 73,764 + GFF58 0.35 -0.1 73,764 + GFF58 0.35 -0.4 73,765 - GFF58 0.35 -1.4 73,765 - GFF58 0.35 +0.6 73,765 - GFF58 0.35 -1.9
Or see this region's nucleotide sequence