Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5289 and GFF5290 are separated by 137 nucleotides GFF5290 and GFF5291 are separated by 208 nucleotides GFF5291 and GFF5292 are separated by 6 nucleotides GFF5292 and GFF5293 are separated by 23 nucleotides
GFF5289 - hypothetical protein, at 160,669 to 161,424
GFF5289
GFF5290 - hypothetical protein, at 161,562 to 161,909
GFF5290
GFF5291 - Ferrichrome-iron receptor, at 162,118 to 164,268
GFF5291
GFF5292 - no description, at 164,275 to 164,778
GFF5292
GFF5293 - N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87), at 164,802 to 166,052
GFF5293
Position (kb)
162
163
164
165 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 161.351 kb on + strand at 161.352 kb on - strand at 161.584 kb on + strand at 161.585 kb on - strand at 161.585 kb on - strand at 161.585 kb on - strand at 161.585 kb on - strand at 161.677 kb on - strand, within GFF5290 at 161.677 kb on - strand, within GFF5290 at 161.727 kb on + strand, within GFF5290 at 161.727 kb on + strand, within GFF5290 at 161.727 kb on + strand, within GFF5290 at 161.727 kb on + strand, within GFF5290 at 161.728 kb on - strand, within GFF5290 at 161.728 kb on - strand, within GFF5290 at 161.728 kb on - strand, within GFF5290 at 161.728 kb on - strand, within GFF5290 at 161.920 kb on + strand at 161.921 kb on - strand at 161.924 kb on - strand at 161.924 kb on - strand at 162.012 kb on - strand at 162.027 kb on + strand at 162.027 kb on + strand at 162.028 kb on - strand at 162.040 kb on + strand at 162.040 kb on + strand at 162.040 kb on + strand at 162.041 kb on - strand at 162.041 kb on - strand at 162.041 kb on - strand at 162.041 kb on - strand at 162.074 kb on - strand at 162.258 kb on + strand at 162.258 kb on + strand at 162.258 kb on + strand at 162.259 kb on - strand at 162.259 kb on - strand at 162.517 kb on + strand, within GFF5291 at 162.517 kb on + strand, within GFF5291 at 162.517 kb on + strand, within GFF5291 at 162.518 kb on - strand, within GFF5291 at 162.520 kb on + strand, within GFF5291 at 162.521 kb on - strand, within GFF5291 at 162.521 kb on - strand, within GFF5291 at 162.521 kb on - strand, within GFF5291 at 162.533 kb on - strand, within GFF5291 at 162.673 kb on + strand, within GFF5291 at 162.673 kb on + strand, within GFF5291 at 162.673 kb on + strand, within GFF5291 at 162.748 kb on + strand, within GFF5291 at 162.748 kb on + strand, within GFF5291 at 162.748 kb on + strand, within GFF5291 at 162.748 kb on + strand, within GFF5291 at 162.749 kb on - strand, within GFF5291 at 162.749 kb on - strand, within GFF5291 at 162.749 kb on - strand, within GFF5291 at 162.749 kb on - strand, within GFF5291 at 162.749 kb on - strand, within GFF5291 at 162.749 kb on - strand, within GFF5291 at 162.766 kb on + strand, within GFF5291 at 162.767 kb on - strand, within GFF5291 at 162.767 kb on - strand, within GFF5291 at 162.854 kb on - strand, within GFF5291 at 162.854 kb on - strand, within GFF5291 at 162.854 kb on - strand, within GFF5291 at 162.877 kb on - strand, within GFF5291 at 163.066 kb on + strand, within GFF5291 at 163.066 kb on + strand, within GFF5291 at 163.066 kb on + strand, within GFF5291 at 163.066 kb on + strand, within GFF5291 at 163.066 kb on + strand, within GFF5291 at 163.067 kb on - strand, within GFF5291 at 163.067 kb on - strand, within GFF5291 at 163.067 kb on - strand, within GFF5291 at 163.126 kb on + strand, within GFF5291 at 163.126 kb on + strand, within GFF5291 at 163.126 kb on + strand, within GFF5291 at 163.126 kb on + strand, within GFF5291 at 163.126 kb on + strand, within GFF5291 at 163.127 kb on - strand, within GFF5291 at 163.127 kb on - strand, within GFF5291 at 163.127 kb on - strand, within GFF5291 at 163.127 kb on - strand, within GFF5291 at 163.127 kb on - strand, within GFF5291 at 163.127 kb on - strand, within GFF5291 at 163.267 kb on + strand, within GFF5291 at 163.393 kb on + strand, within GFF5291 at 163.393 kb on + strand, within GFF5291 at 163.393 kb on + strand, within GFF5291 at 163.393 kb on + strand, within GFF5291 at 163.394 kb on - strand, within GFF5291 at 163.394 kb on - strand, within GFF5291 at 163.394 kb on - strand, within GFF5291 at 163.394 kb on - strand, within GFF5291 at 163.394 kb on - strand, within GFF5291 at 163.450 kb on + strand, within GFF5291 at 163.451 kb on - strand, within GFF5291 at 163.451 kb on - strand, within GFF5291 at 163.543 kb on + strand, within GFF5291 at 163.543 kb on + strand, within GFF5291 at 163.543 kb on + strand, within GFF5291 at 163.544 kb on - strand, within GFF5291 at 163.544 kb on - strand, within GFF5291 at 163.544 kb on - strand, within GFF5291 at 163.544 kb on - strand, within GFF5291 at 163.544 kb on - strand, within GFF5291 at 163.570 kb on + strand, within GFF5291 at 163.570 kb on + strand, within GFF5291 at 163.570 kb on + strand, within GFF5291 at 163.580 kb on - strand, within GFF5291 at 163.580 kb on - strand, within GFF5291 at 163.580 kb on - strand, within GFF5291 at 163.580 kb on - strand, within GFF5291 at 163.580 kb on - strand, within GFF5291 at 163.580 kb on - strand, within GFF5291 at 163.591 kb on + strand, within GFF5291 at 163.669 kb on + strand, within GFF5291 at 163.669 kb on + strand, within GFF5291 at 163.669 kb on + strand, within GFF5291 at 163.726 kb on + strand, within GFF5291 at 163.727 kb on - strand, within GFF5291 at 163.873 kb on + strand, within GFF5291 at 163.873 kb on + strand, within GFF5291 at 163.873 kb on + strand, within GFF5291 at 163.879 kb on + strand, within GFF5291 at 163.879 kb on + strand, within GFF5291 at 163.879 kb on + strand, within GFF5291 at 163.879 kb on + strand, within GFF5291 at 163.880 kb on - strand, within GFF5291 at 163.880 kb on - strand, within GFF5291 at 163.880 kb on - strand, within GFF5291 at 163.979 kb on - strand, within GFF5291 at 163.979 kb on - strand, within GFF5291 at 164.035 kb on + strand, within GFF5291 at 164.035 kb on + strand, within GFF5291 at 164.035 kb on + strand, within GFF5291 at 164.035 kb on + strand, within GFF5291 at 164.035 kb on + strand, within GFF5291 at 164.036 kb on - strand, within GFF5291 at 164.036 kb on - strand, within GFF5291 at 164.098 kb on + strand at 164.185 kb on + strand at 164.311 kb on + strand at 164.311 kb on + strand at 164.312 kb on - strand at 164.312 kb on - strand at 164.458 kb on + strand, within GFF5292 at 164.459 kb on - strand, within GFF5292 at 164.518 kb on + strand, within GFF5292 at 164.518 kb on + strand, within GFF5292 at 164.518 kb on + strand, within GFF5292 at 164.518 kb on + strand, within GFF5292 at 164.519 kb on - strand, within GFF5292 at 164.519 kb on - strand, within GFF5292 at 164.584 kb on + strand, within GFF5292 at 164.584 kb on + strand, within GFF5292 at 164.584 kb on + strand, within GFF5292 at 164.972 kb on + strand, within GFF5293 at 164.972 kb on + strand, within GFF5293
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 161,351 + +0.1 161,352 - +1.0 161,584 + +0.3 161,585 - +1.0 161,585 - -0.4 161,585 - +0.7 161,585 - +0.6 161,677 - GFF5290 0.33 +0.2 161,677 - GFF5290 0.33 -0.6 161,727 + GFF5290 0.47 -0.0 161,727 + GFF5290 0.47 +0.6 161,727 + GFF5290 0.47 -0.9 161,727 + GFF5290 0.47 -0.5 161,728 - GFF5290 0.48 -0.1 161,728 - GFF5290 0.48 +0.2 161,728 - GFF5290 0.48 -1.7 161,728 - GFF5290 0.48 +0.7 161,920 + +0.7 161,921 - -0.2 161,924 - -0.7 161,924 - -0.3 162,012 - -0.3 162,027 + +0.1 162,027 + +0.1 162,028 - +0.5 162,040 + +1.2 162,040 + -1.7 162,040 + +0.8 162,041 - -0.2 162,041 - -1.4 162,041 - -0.1 162,041 - +0.2 162,074 - +0.2 162,258 + -1.1 162,258 + +0.5 162,258 + -0.4 162,259 - -1.2 162,259 - -1.5 162,517 + GFF5291 0.19 -0.2 162,517 + GFF5291 0.19 +0.4 162,517 + GFF5291 0.19 +0.4 162,518 - GFF5291 0.19 +0.5 162,520 + GFF5291 0.19 -0.3 162,521 - GFF5291 0.19 +0.2 162,521 - GFF5291 0.19 -0.5 162,521 - GFF5291 0.19 +0.1 162,533 - GFF5291 0.19 -0.3 162,673 + GFF5291 0.26 +1.0 162,673 + GFF5291 0.26 +0.4 162,673 + GFF5291 0.26 -1.2 162,748 + GFF5291 0.29 +0.0 162,748 + GFF5291 0.29 -0.7 162,748 + GFF5291 0.29 +0.3 162,748 + GFF5291 0.29 -0.5 162,749 - GFF5291 0.29 +1.5 162,749 - GFF5291 0.29 -0.0 162,749 - GFF5291 0.29 +0.3 162,749 - GFF5291 0.29 +0.5 162,749 - GFF5291 0.29 +0.0 162,749 - GFF5291 0.29 +0.2 162,766 + GFF5291 0.30 -2.0 162,767 - GFF5291 0.30 +0.3 162,767 - GFF5291 0.30 -0.2 162,854 - GFF5291 0.34 +0.5 162,854 - GFF5291 0.34 -0.2 162,854 - GFF5291 0.34 +0.8 162,877 - GFF5291 0.35 -1.1 163,066 + GFF5291 0.44 -0.5 163,066 + GFF5291 0.44 -0.5 163,066 + GFF5291 0.44 -0.2 163,066 + GFF5291 0.44 +0.5 163,066 + GFF5291 0.44 -1.9 163,067 - GFF5291 0.44 +0.8 163,067 - GFF5291 0.44 +0.3 163,067 - GFF5291 0.44 -0.2 163,126 + GFF5291 0.47 +0.6 163,126 + GFF5291 0.47 +0.8 163,126 + GFF5291 0.47 +0.1 163,126 + GFF5291 0.47 +1.5 163,126 + GFF5291 0.47 +0.1 163,127 - GFF5291 0.47 +0.3 163,127 - GFF5291 0.47 -0.6 163,127 - GFF5291 0.47 -0.3 163,127 - GFF5291 0.47 +1.0 163,127 - GFF5291 0.47 -2.0 163,127 - GFF5291 0.47 -1.5 163,267 + GFF5291 0.53 +0.6 163,393 + GFF5291 0.59 -1.2 163,393 + GFF5291 0.59 -0.2 163,393 + GFF5291 0.59 +0.1 163,393 + GFF5291 0.59 +0.1 163,394 - GFF5291 0.59 +0.9 163,394 - GFF5291 0.59 -0.5 163,394 - GFF5291 0.59 +1.0 163,394 - GFF5291 0.59 -1.1 163,394 - GFF5291 0.59 +0.3 163,450 + GFF5291 0.62 -0.0 163,451 - GFF5291 0.62 +0.7 163,451 - GFF5291 0.62 -1.2 163,543 + GFF5291 0.66 -0.7 163,543 + GFF5291 0.66 +1.6 163,543 + GFF5291 0.66 +0.8 163,544 - GFF5291 0.66 +0.2 163,544 - GFF5291 0.66 -0.6 163,544 - GFF5291 0.66 -0.7 163,544 - GFF5291 0.66 +0.3 163,544 - GFF5291 0.66 +1.0 163,570 + GFF5291 0.68 -0.7 163,570 + GFF5291 0.68 -1.0 163,570 + GFF5291 0.68 +0.8 163,580 - GFF5291 0.68 -0.7 163,580 - GFF5291 0.68 +0.0 163,580 - GFF5291 0.68 +0.2 163,580 - GFF5291 0.68 -0.3 163,580 - GFF5291 0.68 +0.2 163,580 - GFF5291 0.68 +0.3 163,591 + GFF5291 0.68 -2.4 163,669 + GFF5291 0.72 +0.1 163,669 + GFF5291 0.72 -0.5 163,669 + GFF5291 0.72 +0.0 163,726 + GFF5291 0.75 +0.2 163,727 - GFF5291 0.75 -0.0 163,873 + GFF5291 0.82 +0.7 163,873 + GFF5291 0.82 -0.7 163,873 + GFF5291 0.82 +0.6 163,879 + GFF5291 0.82 +0.4 163,879 + GFF5291 0.82 -0.0 163,879 + GFF5291 0.82 -0.5 163,879 + GFF5291 0.82 +0.5 163,880 - GFF5291 0.82 +1.0 163,880 - GFF5291 0.82 -1.3 163,880 - GFF5291 0.82 +0.3 163,979 - GFF5291 0.87 -0.2 163,979 - GFF5291 0.87 +1.3 164,035 + GFF5291 0.89 -0.0 164,035 + GFF5291 0.89 +0.4 164,035 + GFF5291 0.89 +0.1 164,035 + GFF5291 0.89 -1.7 164,035 + GFF5291 0.89 +1.8 164,036 - GFF5291 0.89 -1.2 164,036 - GFF5291 0.89 -0.7 164,098 + +0.1 164,185 + -0.8 164,311 + -1.3 164,311 + +0.3 164,312 - +0.2 164,312 - -0.7 164,458 + GFF5292 0.36 +1.0 164,459 - GFF5292 0.37 +0.1 164,518 + GFF5292 0.48 -0.0 164,518 + GFF5292 0.48 +0.3 164,518 + GFF5292 0.48 +2.6 164,518 + GFF5292 0.48 -2.5 164,519 - GFF5292 0.48 +1.0 164,519 - GFF5292 0.48 -0.1 164,584 + GFF5292 0.61 +0.8 164,584 + GFF5292 0.61 -0.0 164,584 + GFF5292 0.61 -0.0 164,972 + GFF5293 0.14 -0.6 164,972 + GFF5293 0.14 +0.8
Or see this region's nucleotide sequence