Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5276 and GFF5277 overlap by 4 nucleotides GFF5277 and GFF5278 are separated by 22 nucleotides GFF5278 and GFF5279 are separated by 30 nucleotides
GFF5276 - D-arabinitol 4-dehydrogenase (EC 1.1.1.11), at 144,433 to 145,869
GFF5276
GFF5277 - Sorbitol dehydrogenase (EC 1.1.1.14), at 145,866 to 146,654
GFF5277
GFF5278 - Transaldolase (EC 2.2.1.2), at 146,677 to 147,348
GFF5278
GFF5279 - Fructokinase (EC 2.7.1.4), at 147,379 to 148,347
GFF5279
Position (kb) 145
146
147 Strain fitness (log2 ratio) -2
-1
0
1
2 at 144.898 kb on + strand, within GFF5276 at 144.898 kb on + strand, within GFF5276 at 144.899 kb on - strand, within GFF5276 at 144.899 kb on - strand, within GFF5276 at 144.899 kb on - strand, within GFF5276 at 145.276 kb on + strand, within GFF5276 at 145.276 kb on + strand, within GFF5276 at 145.276 kb on + strand, within GFF5276 at 145.277 kb on - strand, within GFF5276 at 145.277 kb on - strand, within GFF5276 at 145.277 kb on - strand, within GFF5276 at 145.405 kb on + strand, within GFF5276 at 145.405 kb on + strand, within GFF5276 at 145.405 kb on + strand, within GFF5276 at 145.406 kb on - strand, within GFF5276 at 145.406 kb on - strand, within GFF5276 at 145.673 kb on - strand, within GFF5276 at 145.678 kb on + strand, within GFF5276 at 145.679 kb on - strand, within GFF5276 at 145.679 kb on - strand, within GFF5276 at 146.043 kb on + strand, within GFF5277 at 146.043 kb on + strand, within GFF5277 at 146.043 kb on + strand, within GFF5277 at 146.044 kb on - strand, within GFF5277 at 146.044 kb on - strand, within GFF5277 at 146.044 kb on - strand, within GFF5277 at 146.044 kb on - strand, within GFF5277 at 146.044 kb on - strand, within GFF5277 at 146.337 kb on + strand, within GFF5277 at 146.337 kb on + strand, within GFF5277 at 146.337 kb on + strand, within GFF5277 at 146.367 kb on + strand, within GFF5277 at 146.367 kb on + strand, within GFF5277 at 146.367 kb on + strand, within GFF5277 at 146.367 kb on + strand, within GFF5277 at 146.367 kb on + strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.368 kb on - strand, within GFF5277 at 146.407 kb on + strand, within GFF5277 at 146.407 kb on + strand, within GFF5277 at 146.408 kb on - strand, within GFF5277 at 146.408 kb on - strand, within GFF5277 at 146.604 kb on + strand at 146.605 kb on - strand at 146.698 kb on + strand at 146.699 kb on - strand at 146.699 kb on - strand at 146.699 kb on - strand at 146.699 kb on - strand at 147.031 kb on + strand, within GFF5278 at 147.032 kb on - strand, within GFF5278 at 147.077 kb on - strand, within GFF5278 at 147.077 kb on - strand, within GFF5278 at 147.088 kb on + strand, within GFF5278 at 147.217 kb on + strand, within GFF5278 at 147.217 kb on + strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.218 kb on - strand, within GFF5278 at 147.218 kb on - strand, within GFF5278
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 144,898 + GFF5276 0.32 +0.2 144,898 + GFF5276 0.32 -0.4 144,899 - GFF5276 0.32 +0.6 144,899 - GFF5276 0.32 -2.5 144,899 - GFF5276 0.32 -1.1 145,276 + GFF5276 0.59 -1.7 145,276 + GFF5276 0.59 -0.2 145,276 + GFF5276 0.59 -0.2 145,277 - GFF5276 0.59 -1.1 145,277 - GFF5276 0.59 -0.7 145,277 - GFF5276 0.59 -0.4 145,405 + GFF5276 0.68 +1.8 145,405 + GFF5276 0.68 -0.1 145,405 + GFF5276 0.68 -0.5 145,406 - GFF5276 0.68 +0.8 145,406 - GFF5276 0.68 -0.7 145,673 - GFF5276 0.86 +0.2 145,678 + GFF5276 0.87 -0.2 145,679 - GFF5276 0.87 -0.4 145,679 - GFF5276 0.87 -0.0 146,043 + GFF5277 0.22 -0.1 146,043 + GFF5277 0.22 +0.7 146,043 + GFF5277 0.22 -1.5 146,044 - GFF5277 0.23 -0.5 146,044 - GFF5277 0.23 -0.5 146,044 - GFF5277 0.23 -0.8 146,044 - GFF5277 0.23 -1.2 146,044 - GFF5277 0.23 -0.5 146,337 + GFF5277 0.60 -2.3 146,337 + GFF5277 0.60 -1.1 146,337 + GFF5277 0.60 +1.6 146,367 + GFF5277 0.63 -0.0 146,367 + GFF5277 0.63 -1.7 146,367 + GFF5277 0.63 -0.0 146,367 + GFF5277 0.63 +1.2 146,367 + GFF5277 0.63 -0.6 146,368 - GFF5277 0.64 +1.9 146,368 - GFF5277 0.64 -0.4 146,368 - GFF5277 0.64 -1.9 146,368 - GFF5277 0.64 -1.4 146,368 - GFF5277 0.64 -0.9 146,368 - GFF5277 0.64 +0.6 146,407 + GFF5277 0.69 -0.9 146,407 + GFF5277 0.69 -0.2 146,408 - GFF5277 0.69 -1.4 146,408 - GFF5277 0.69 -0.2 146,604 + +0.2 146,605 - +0.2 146,698 + +0.4 146,699 - +0.2 146,699 - -0.4 146,699 - +0.8 146,699 - +0.4 147,031 + GFF5278 0.53 -0.8 147,032 - GFF5278 0.53 -0.9 147,077 - GFF5278 0.60 +0.5 147,077 - GFF5278 0.60 -0.4 147,088 + GFF5278 0.61 -0.1 147,217 + GFF5278 0.80 +0.4 147,217 + GFF5278 0.80 -0.6 147,218 - GFF5278 0.81 -0.0 147,218 - GFF5278 0.81 -0.7 147,218 - GFF5278 0.81 -0.4 147,218 - GFF5278 0.81 +0.6
Or see this region's nucleotide sequence