Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5157 and GFF5158 overlap by 4 nucleotides GFF5158 and GFF5159 are separated by 58 nucleotides
GFF5157 - Glucoamylase (EC 3.2.1.3), at 9,614 to 11,482
GFF5157
GFF5158 - Trehalose-6-phosphate phosphatase (EC 3.1.3.12), at 11,479 to 12,297
GFF5158
GFF5159 - CCA tRNA nucleotidyltransferase (EC 2.7.7.72), at 12,356 to 13,615
GFF5159
Position (kb)
11
12
13 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 10.479 kb on + strand, within GFF5157 at 10.479 kb on + strand, within GFF5157 at 10.479 kb on + strand, within GFF5157 at 10.480 kb on - strand, within GFF5157 at 10.480 kb on - strand, within GFF5157 at 10.480 kb on - strand, within GFF5157 at 10.480 kb on - strand, within GFF5157 at 10.480 kb on - strand, within GFF5157 at 10.539 kb on + strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.614 kb on + strand, within GFF5157 at 10.615 kb on - strand, within GFF5157 at 10.620 kb on + strand, within GFF5157 at 10.620 kb on + strand, within GFF5157 at 10.620 kb on + strand, within GFF5157 at 10.621 kb on - strand, within GFF5157 at 10.621 kb on - strand, within GFF5157 at 10.621 kb on - strand, within GFF5157 at 10.698 kb on + strand, within GFF5157 at 10.699 kb on - strand, within GFF5157 at 10.699 kb on - strand, within GFF5157 at 10.746 kb on + strand, within GFF5157 at 10.747 kb on - strand, within GFF5157 at 10.747 kb on - strand, within GFF5157 at 10.747 kb on - strand, within GFF5157 at 10.789 kb on - strand, within GFF5157 at 10.872 kb on + strand, within GFF5157 at 10.872 kb on + strand, within GFF5157 at 10.872 kb on + strand, within GFF5157 at 10.872 kb on + strand, within GFF5157 at 10.872 kb on + strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.962 kb on + strand, within GFF5157 at 10.962 kb on + strand, within GFF5157 at 10.963 kb on - strand, within GFF5157 at 10.963 kb on - strand, within GFF5157 at 10.963 kb on - strand, within GFF5157 at 10.963 kb on - strand, within GFF5157 at 11.115 kb on + strand, within GFF5157 at 11.115 kb on + strand, within GFF5157 at 11.115 kb on + strand, within GFF5157 at 11.116 kb on - strand, within GFF5157 at 11.116 kb on - strand, within GFF5157 at 11.116 kb on - strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.203 kb on - strand, within GFF5157 at 11.203 kb on - strand, within GFF5157 at 11.203 kb on - strand, within GFF5157 at 11.203 kb on - strand, within GFF5157 at 11.203 kb on - strand, within GFF5157 at 11.355 kb on + strand at 11.355 kb on + strand at 11.355 kb on + strand at 11.355 kb on + strand at 11.355 kb on + strand at 11.356 kb on - strand at 11.356 kb on - strand at 11.567 kb on + strand, within GFF5158 at 11.567 kb on + strand, within GFF5158 at 11.567 kb on + strand, within GFF5158 at 11.568 kb on - strand, within GFF5158 at 11.568 kb on - strand, within GFF5158 at 12.201 kb on + strand, within GFF5158 at 12.201 kb on + strand, within GFF5158 at 12.202 kb on - strand, within GFF5158 at 12.202 kb on - strand, within GFF5158 at 12.202 kb on - strand, within GFF5158 at 12.202 kb on - strand, within GFF5158 at 12.292 kb on + strand at 12.292 kb on + strand at 12.292 kb on + strand at 12.292 kb on + strand at 12.292 kb on + strand at 12.292 kb on + strand at 12.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 10,479 + GFF5157 0.46 +0.3 10,479 + GFF5157 0.46 -2.0 10,479 + GFF5157 0.46 +0.2 10,480 - GFF5157 0.46 +0.2 10,480 - GFF5157 0.46 -0.0 10,480 - GFF5157 0.46 -1.2 10,480 - GFF5157 0.46 -0.2 10,480 - GFF5157 0.46 -0.5 10,539 + GFF5157 0.49 +1.1 10,540 - GFF5157 0.50 +2.6 10,540 - GFF5157 0.50 -0.5 10,540 - GFF5157 0.50 -0.0 10,540 - GFF5157 0.50 +0.2 10,540 - GFF5157 0.50 -0.1 10,540 - GFF5157 0.50 -0.4 10,540 - GFF5157 0.50 -0.3 10,614 + GFF5157 0.54 -0.0 10,615 - GFF5157 0.54 +0.1 10,620 + GFF5157 0.54 -0.4 10,620 + GFF5157 0.54 -0.0 10,620 + GFF5157 0.54 -0.6 10,621 - GFF5157 0.54 -0.7 10,621 - GFF5157 0.54 -1.8 10,621 - GFF5157 0.54 -1.6 10,698 + GFF5157 0.58 +0.3 10,699 - GFF5157 0.58 -0.2 10,699 - GFF5157 0.58 -0.2 10,746 + GFF5157 0.61 -0.1 10,747 - GFF5157 0.61 -0.7 10,747 - GFF5157 0.61 +0.4 10,747 - GFF5157 0.61 +0.2 10,789 - GFF5157 0.63 +0.2 10,872 + GFF5157 0.67 +0.2 10,872 + GFF5157 0.67 +0.1 10,872 + GFF5157 0.67 -0.7 10,872 + GFF5157 0.67 -0.2 10,872 + GFF5157 0.67 -0.3 10,873 - GFF5157 0.67 -0.9 10,873 - GFF5157 0.67 -0.6 10,873 - GFF5157 0.67 +0.2 10,873 - GFF5157 0.67 -0.8 10,873 - GFF5157 0.67 -1.1 10,873 - GFF5157 0.67 -1.0 10,873 - GFF5157 0.67 -2.0 10,962 + GFF5157 0.72 -0.4 10,962 + GFF5157 0.72 +0.4 10,963 - GFF5157 0.72 +0.2 10,963 - GFF5157 0.72 +0.5 10,963 - GFF5157 0.72 -0.6 10,963 - GFF5157 0.72 -0.8 11,115 + GFF5157 0.80 -0.8 11,115 + GFF5157 0.80 -1.3 11,115 + GFF5157 0.80 +0.7 11,116 - GFF5157 0.80 +0.5 11,116 - GFF5157 0.80 +0.8 11,116 - GFF5157 0.80 +1.5 11,202 + GFF5157 0.85 -0.7 11,202 + GFF5157 0.85 +0.5 11,202 + GFF5157 0.85 -1.2 11,202 + GFF5157 0.85 +0.4 11,202 + GFF5157 0.85 -3.1 11,202 + GFF5157 0.85 -0.1 11,202 + GFF5157 0.85 -0.7 11,202 + GFF5157 0.85 -1.8 11,203 - GFF5157 0.85 -0.3 11,203 - GFF5157 0.85 +1.1 11,203 - GFF5157 0.85 -0.7 11,203 - GFF5157 0.85 -1.5 11,203 - GFF5157 0.85 -0.9 11,355 + -0.0 11,355 + -0.6 11,355 + -1.0 11,355 + -1.0 11,355 + +0.1 11,356 - -0.2 11,356 - +0.8 11,567 + GFF5158 0.11 -1.2 11,567 + GFF5158 0.11 -2.7 11,567 + GFF5158 0.11 -1.5 11,568 - GFF5158 0.11 -3.5 11,568 - GFF5158 0.11 -3.6 12,201 + GFF5158 0.88 -2.0 12,201 + GFF5158 0.88 -3.2 12,202 - GFF5158 0.88 -2.1 12,202 - GFF5158 0.88 -3.6 12,202 - GFF5158 0.88 -5.5 12,202 - GFF5158 0.88 -1.2 12,292 + -0.6 12,292 + +0.3 12,292 + +0.3 12,292 + +0.2 12,292 + -0.4 12,292 + -0.5 12,293 - -0.0
Or see this region's nucleotide sequence