Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5115 and GFF5116 overlap by 1 nucleotides GFF5116 and GFF5117 are separated by 126 nucleotides
GFF5115 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 216,115 to 217,233
GFF5115
GFF5116 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 217,233 to 218,735
GFF5116
GFF5117 - Transcriptional regulator, LysR family, at 218,862 to 219,746
GFF5117
Position (kb)
217
218
219 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 216.332 kb on + strand, within GFF5115 at 216.332 kb on + strand, within GFF5115 at 216.332 kb on + strand, within GFF5115 at 216.332 kb on + strand, within GFF5115 at 216.333 kb on - strand, within GFF5115 at 216.575 kb on + strand, within GFF5115 at 216.576 kb on - strand, within GFF5115 at 216.576 kb on - strand, within GFF5115 at 216.576 kb on - strand, within GFF5115 at 216.576 kb on - strand, within GFF5115 at 216.597 kb on - strand, within GFF5115 at 216.597 kb on - strand, within GFF5115 at 216.597 kb on - strand, within GFF5115 at 216.597 kb on - strand, within GFF5115 at 216.869 kb on + strand, within GFF5115 at 216.869 kb on + strand, within GFF5115 at 216.870 kb on - strand, within GFF5115 at 216.870 kb on - strand, within GFF5115 at 216.870 kb on - strand, within GFF5115 at 217.064 kb on + strand, within GFF5115 at 217.064 kb on + strand, within GFF5115 at 217.065 kb on - strand, within GFF5115 at 217.065 kb on - strand, within GFF5115 at 217.124 kb on + strand at 217.125 kb on - strand at 217.198 kb on + strand at 217.199 kb on - strand at 217.199 kb on - strand at 217.199 kb on - strand at 217.604 kb on - strand, within GFF5116 at 217.681 kb on + strand, within GFF5116 at 217.681 kb on + strand, within GFF5116 at 217.681 kb on + strand, within GFF5116 at 217.681 kb on + strand, within GFF5116 at 217.681 kb on + strand, within GFF5116 at 217.681 kb on + strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.682 kb on - strand, within GFF5116 at 217.885 kb on - strand, within GFF5116 at 217.886 kb on - strand, within GFF5116 at 218.302 kb on + strand, within GFF5116 at 218.303 kb on - strand, within GFF5116 at 218.303 kb on - strand, within GFF5116 at 218.305 kb on + strand, within GFF5116 at 218.305 kb on + strand, within GFF5116 at 218.305 kb on + strand, within GFF5116 at 218.305 kb on + strand, within GFF5116 at 218.305 kb on + strand, within GFF5116 at 218.306 kb on - strand, within GFF5116 at 218.306 kb on - strand, within GFF5116 at 218.306 kb on - strand, within GFF5116 at 218.306 kb on - strand, within GFF5116 at 218.779 kb on + strand at 218.779 kb on + strand at 218.779 kb on + strand at 218.779 kb on + strand at 218.779 kb on + strand at 218.780 kb on - strand at 218.780 kb on - strand at 218.780 kb on - strand at 218.780 kb on - strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.789 kb on + strand at 218.790 kb on - strand at 218.790 kb on - strand at 218.790 kb on - strand at 218.941 kb on + strand at 218.941 kb on + strand at 218.941 kb on + strand at 218.941 kb on + strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 218.942 kb on - strand at 219.064 kb on + strand, within GFF5117 at 219.064 kb on + strand, within GFF5117 at 219.064 kb on + strand, within GFF5117 at 219.064 kb on + strand, within GFF5117 at 219.064 kb on + strand, within GFF5117 at 219.065 kb on - strand, within GFF5117 at 219.065 kb on - strand, within GFF5117 at 219.065 kb on - strand, within GFF5117 at 219.065 kb on - strand, within GFF5117 at 219.065 kb on - strand, within GFF5117 at 219.232 kb on + strand, within GFF5117 at 219.232 kb on + strand, within GFF5117 at 219.232 kb on + strand, within GFF5117 at 219.232 kb on + strand, within GFF5117 at 219.233 kb on - strand, within GFF5117 at 219.233 kb on - strand, within GFF5117 at 219.233 kb on - strand, within GFF5117 at 219.233 kb on - strand, within GFF5117 at 219.233 kb on - strand, within GFF5117 at 219.233 kb on - strand, within GFF5117 at 219.392 kb on - strand, within GFF5117 at 219.392 kb on - strand, within GFF5117 at 219.392 kb on - strand, within GFF5117 at 219.392 kb on - strand, within GFF5117 at 219.392 kb on - strand, within GFF5117 at 219.392 kb on - strand, within GFF5117 at 219.392 kb on - strand, within GFF5117 at 219.544 kb on + strand, within GFF5117
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 216,332 + GFF5115 0.19 -0.7 216,332 + GFF5115 0.19 +0.1 216,332 + GFF5115 0.19 -0.5 216,332 + GFF5115 0.19 -0.7 216,333 - GFF5115 0.19 -0.2 216,575 + GFF5115 0.41 +0.4 216,576 - GFF5115 0.41 -1.2 216,576 - GFF5115 0.41 +0.2 216,576 - GFF5115 0.41 -1.5 216,576 - GFF5115 0.41 +0.9 216,597 - GFF5115 0.43 -0.2 216,597 - GFF5115 0.43 -0.1 216,597 - GFF5115 0.43 +1.6 216,597 - GFF5115 0.43 +0.8 216,869 + GFF5115 0.67 +0.3 216,869 + GFF5115 0.67 -0.2 216,870 - GFF5115 0.67 +0.1 216,870 - GFF5115 0.67 +0.6 216,870 - GFF5115 0.67 -0.6 217,064 + GFF5115 0.85 -1.1 217,064 + GFF5115 0.85 -0.1 217,065 - GFF5115 0.85 -1.8 217,065 - GFF5115 0.85 +0.4 217,124 + -1.5 217,125 - -1.0 217,198 + +0.1 217,199 - -0.2 217,199 - +0.9 217,199 - -0.7 217,604 - GFF5116 0.25 -1.6 217,681 + GFF5116 0.30 -0.2 217,681 + GFF5116 0.30 -0.2 217,681 + GFF5116 0.30 -0.0 217,681 + GFF5116 0.30 -0.6 217,681 + GFF5116 0.30 -1.3 217,681 + GFF5116 0.30 -0.5 217,682 - GFF5116 0.30 -1.1 217,682 - GFF5116 0.30 +0.6 217,682 - GFF5116 0.30 +1.1 217,682 - GFF5116 0.30 -0.9 217,682 - GFF5116 0.30 -1.3 217,682 - GFF5116 0.30 -0.2 217,682 - GFF5116 0.30 -0.8 217,682 - GFF5116 0.30 -0.4 217,682 - GFF5116 0.30 -0.4 217,682 - GFF5116 0.30 -1.4 217,682 - GFF5116 0.30 +1.1 217,682 - GFF5116 0.30 +0.4 217,682 - GFF5116 0.30 +0.2 217,682 - GFF5116 0.30 -1.5 217,682 - GFF5116 0.30 -0.4 217,682 - GFF5116 0.30 +0.1 217,885 - GFF5116 0.43 +1.0 217,886 - GFF5116 0.43 -0.8 218,302 + GFF5116 0.71 -0.5 218,303 - GFF5116 0.71 -0.8 218,303 - GFF5116 0.71 -0.1 218,305 + GFF5116 0.71 -0.6 218,305 + GFF5116 0.71 +0.5 218,305 + GFF5116 0.71 +0.2 218,305 + GFF5116 0.71 -0.8 218,305 + GFF5116 0.71 -0.2 218,306 - GFF5116 0.71 -1.0 218,306 - GFF5116 0.71 +1.1 218,306 - GFF5116 0.71 +0.9 218,306 - GFF5116 0.71 -0.2 218,779 + -0.6 218,779 + -0.9 218,779 + -0.2 218,779 + -0.5 218,779 + -0.3 218,780 - -0.4 218,780 - -1.3 218,780 - -0.2 218,780 - +0.8 218,789 + -0.8 218,789 + -1.8 218,789 + -0.7 218,789 + +0.1 218,790 - -0.4 218,790 - -0.0 218,790 - -0.3 218,941 + -0.3 218,941 + -1.1 218,941 + +0.5 218,941 + -1.4 218,942 - -0.5 218,942 - -1.2 218,942 - +0.9 218,942 - -0.1 218,942 - -0.4 218,942 - -0.8 218,942 - +0.0 218,942 - +0.1 218,942 - +0.1 218,942 - +0.5 218,942 - +0.7 218,942 - +1.0 218,942 - +0.8 218,942 - -1.6 219,064 + GFF5117 0.23 +0.3 219,064 + GFF5117 0.23 +1.3 219,064 + GFF5117 0.23 -0.2 219,064 + GFF5117 0.23 -0.3 219,064 + GFF5117 0.23 +0.4 219,065 - GFF5117 0.23 +1.9 219,065 - GFF5117 0.23 -0.2 219,065 - GFF5117 0.23 -0.8 219,065 - GFF5117 0.23 -0.2 219,065 - GFF5117 0.23 +0.0 219,232 + GFF5117 0.42 -1.2 219,232 + GFF5117 0.42 +0.8 219,232 + GFF5117 0.42 -0.7 219,232 + GFF5117 0.42 -0.1 219,233 - GFF5117 0.42 -0.7 219,233 - GFF5117 0.42 +0.9 219,233 - GFF5117 0.42 -0.2 219,233 - GFF5117 0.42 -0.3 219,233 - GFF5117 0.42 -0.0 219,233 - GFF5117 0.42 -1.4 219,392 - GFF5117 0.60 -0.1 219,392 - GFF5117 0.60 -0.7 219,392 - GFF5117 0.60 -2.9 219,392 - GFF5117 0.60 -0.1 219,392 - GFF5117 0.60 +0.3 219,392 - GFF5117 0.60 +0.5 219,392 - GFF5117 0.60 +0.3 219,544 + GFF5117 0.77 +0.3
Or see this region's nucleotide sequence