Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5093 and GFF5094 are separated by 2 nucleotides GFF5094 and GFF5095 are separated by 3 nucleotides GFF5095 and GFF5096 are separated by 85 nucleotides GFF5096 and GFF5097 are separated by 13 nucleotides
GFF5093 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 195,580 to 197,043
GFF5093
GFF5094 - BUG/TctC family periplasmic protein, at 197,046 to 198,014
GFF5094
GFF5095 - hypothetical protein, at 198,018 to 198,353
GFF5095
GFF5096 - similar to protocatechuate 4,5-dioxygenase beta chain, at 198,439 to 199,266
GFF5096
GFF5097 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 199,280 to 199,687
GFF5097
Position (kb) 198
199 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 197.304 kb on + strand, within GFF5094 at 197.304 kb on + strand, within GFF5094 at 197.368 kb on - strand, within GFF5094 at 197.593 kb on - strand, within GFF5094 at 197.593 kb on - strand, within GFF5094 at 197.770 kb on + strand, within GFF5094 at 197.771 kb on - strand, within GFF5094 at 197.771 kb on - strand, within GFF5094 at 197.970 kb on + strand at 198.183 kb on + strand, within GFF5095 at 198.183 kb on + strand, within GFF5095 at 198.183 kb on + strand, within GFF5095 at 198.183 kb on + strand, within GFF5095 at 198.184 kb on - strand, within GFF5095 at 198.184 kb on - strand, within GFF5095 at 198.252 kb on + strand, within GFF5095 at 198.285 kb on + strand, within GFF5095 at 198.286 kb on - strand, within GFF5095 at 198.286 kb on - strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.310 kb on - strand, within GFF5095 at 198.310 kb on - strand, within GFF5095 at 198.310 kb on - strand, within GFF5095 at 198.349 kb on + strand at 198.450 kb on - strand at 198.640 kb on + strand, within GFF5096 at 198.664 kb on + strand, within GFF5096 at 198.664 kb on + strand, within GFF5096 at 198.665 kb on - strand, within GFF5096 at 198.665 kb on - strand, within GFF5096 at 198.758 kb on + strand, within GFF5096 at 198.758 kb on + strand, within GFF5096 at 199.120 kb on + strand, within GFF5096 at 199.120 kb on + strand, within GFF5096 at 199.121 kb on - strand, within GFF5096 at 199.301 kb on + strand at 199.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 197,304 + GFF5094 0.27 -0.4 197,304 + GFF5094 0.27 -0.2 197,368 - GFF5094 0.33 +0.3 197,593 - GFF5094 0.56 -2.1 197,593 - GFF5094 0.56 -0.5 197,770 + GFF5094 0.75 +0.5 197,771 - GFF5094 0.75 -0.2 197,771 - GFF5094 0.75 +1.9 197,970 + -1.1 198,183 + GFF5095 0.49 -1.0 198,183 + GFF5095 0.49 +0.1 198,183 + GFF5095 0.49 +0.4 198,183 + GFF5095 0.49 +0.9 198,184 - GFF5095 0.49 +0.9 198,184 - GFF5095 0.49 +0.5 198,252 + GFF5095 0.70 +0.7 198,285 + GFF5095 0.79 +0.3 198,286 - GFF5095 0.80 -1.7 198,286 - GFF5095 0.80 +0.6 198,309 + GFF5095 0.87 +3.7 198,309 + GFF5095 0.87 -0.3 198,309 + GFF5095 0.87 +0.0 198,309 + GFF5095 0.87 -1.8 198,309 + GFF5095 0.87 -0.3 198,309 + GFF5095 0.87 -1.9 198,309 + GFF5095 0.87 -2.3 198,309 + GFF5095 0.87 +0.4 198,310 - GFF5095 0.87 -0.5 198,310 - GFF5095 0.87 +0.4 198,310 - GFF5095 0.87 -0.1 198,349 + +0.7 198,450 - +0.1 198,640 + GFF5096 0.24 -1.4 198,664 + GFF5096 0.27 -0.3 198,664 + GFF5096 0.27 -0.4 198,665 - GFF5096 0.27 -0.8 198,665 - GFF5096 0.27 -0.3 198,758 + GFF5096 0.39 +1.3 198,758 + GFF5096 0.39 +0.4 199,120 + GFF5096 0.82 +2.6 199,120 + GFF5096 0.82 -0.1 199,121 - GFF5096 0.82 -0.7 199,301 + -1.2 199,302 - -0.7
Or see this region's nucleotide sequence