Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5041 and GFF5042 are separated by 119 nucleotides GFF5042 and GFF5043 are separated by 182 nucleotides
GFF5041 - Malonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.18), at 142,186 to 143,697
GFF5041
GFF5042 - Transcriptional regulator, LysR family, at 143,817 to 144,749
GFF5042
GFF5043 - ABC transporter, permease protein (cluster 9, phospholipid), at 144,932 to 146,098
GFF5043
Position (kb) 143
144
145 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 142.992 kb on - strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.997 kb on + strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 142.998 kb on - strand, within GFF5041 at 143.746 kb on + strand at 143.746 kb on + strand at 143.769 kb on + strand at 143.770 kb on - strand at 143.770 kb on - strand at 143.788 kb on + strand at 143.789 kb on - strand at 143.789 kb on - strand at 143.832 kb on - strand at 144.345 kb on + strand, within GFF5042 at 144.345 kb on + strand, within GFF5042 at 144.346 kb on - strand, within GFF5042 at 144.346 kb on - strand, within GFF5042 at 144.654 kb on + strand, within GFF5042 at 144.654 kb on + strand, within GFF5042 at 144.654 kb on + strand, within GFF5042 at 144.669 kb on + strand at 144.670 kb on - strand at 144.670 kb on - strand at 144.755 kb on + strand at 144.755 kb on + strand at 144.755 kb on + strand at 144.756 kb on - strand at 144.756 kb on - strand at 144.801 kb on - strand at 144.832 kb on - strand at 144.859 kb on + strand at 144.859 kb on + strand at 144.859 kb on + strand at 144.860 kb on - strand at 144.860 kb on - strand at 144.878 kb on + strand at 144.879 kb on - strand at 144.911 kb on - strand at 144.911 kb on - strand at 144.911 kb on - strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.932 kb on - strand at 144.932 kb on - strand at 144.932 kb on - strand at 144.932 kb on - strand at 144.932 kb on - strand at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.451 kb on + strand, within GFF5043 at 145.452 kb on - strand, within GFF5043 at 145.452 kb on - strand, within GFF5043 at 145.452 kb on - strand, within GFF5043 at 145.497 kb on + strand, within GFF5043 at 145.498 kb on - strand, within GFF5043 at 145.498 kb on - strand, within GFF5043 at 145.520 kb on + strand, within GFF5043 at 145.521 kb on - strand, within GFF5043 at 145.521 kb on - strand, within GFF5043 at 145.521 kb on - strand, within GFF5043 at 145.521 kb on - strand, within GFF5043 at 145.716 kb on + strand, within GFF5043 at 145.716 kb on + strand, within GFF5043
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 142,992 - GFF5041 0.53 +0.6 142,997 + GFF5041 0.54 +0.7 142,997 + GFF5041 0.54 +0.2 142,997 + GFF5041 0.54 +0.0 142,997 + GFF5041 0.54 -0.1 142,997 + GFF5041 0.54 +0.0 142,997 + GFF5041 0.54 -0.2 142,997 + GFF5041 0.54 +0.4 142,997 + GFF5041 0.54 +1.2 142,997 + GFF5041 0.54 +1.0 142,997 + GFF5041 0.54 -0.1 142,997 + GFF5041 0.54 +0.7 142,997 + GFF5041 0.54 -0.5 142,997 + GFF5041 0.54 -0.3 142,997 + GFF5041 0.54 +1.3 142,997 + GFF5041 0.54 +0.3 142,997 + GFF5041 0.54 +0.3 142,997 + GFF5041 0.54 -0.6 142,997 + GFF5041 0.54 +1.3 142,998 - GFF5041 0.54 -0.5 142,998 - GFF5041 0.54 +0.4 142,998 - GFF5041 0.54 +1.5 142,998 - GFF5041 0.54 -0.5 142,998 - GFF5041 0.54 -1.4 142,998 - GFF5041 0.54 +0.6 142,998 - GFF5041 0.54 -1.3 142,998 - GFF5041 0.54 +0.3 142,998 - GFF5041 0.54 +0.8 142,998 - GFF5041 0.54 -0.7 142,998 - GFF5041 0.54 +0.3 142,998 - GFF5041 0.54 -0.7 142,998 - GFF5041 0.54 -0.6 142,998 - GFF5041 0.54 -0.5 142,998 - GFF5041 0.54 -1.8 143,746 + -1.2 143,746 + -1.2 143,769 + -1.4 143,770 - -0.1 143,770 - -1.4 143,788 + -0.5 143,789 - -1.6 143,789 - -3.1 143,832 - -0.4 144,345 + GFF5042 0.57 -1.7 144,345 + GFF5042 0.57 -1.0 144,346 - GFF5042 0.57 -1.2 144,346 - GFF5042 0.57 -1.5 144,654 + GFF5042 0.90 -0.1 144,654 + GFF5042 0.90 -1.4 144,654 + GFF5042 0.90 -0.1 144,669 + -1.9 144,670 - -1.3 144,670 - -0.5 144,755 + -1.0 144,755 + -0.5 144,755 + +0.2 144,756 - +1.0 144,756 - +0.5 144,801 - +0.5 144,832 - +0.5 144,859 + +0.3 144,859 + +0.6 144,859 + +0.7 144,860 - -0.8 144,860 - -1.0 144,878 + +0.1 144,879 - -0.8 144,911 - +0.7 144,911 - -0.7 144,911 - -1.4 144,931 + -0.2 144,931 + +0.9 144,931 + -0.5 144,931 + -0.2 144,931 + -1.2 144,931 + -1.1 144,931 + +0.1 144,931 + -0.4 144,931 + -0.2 144,931 + -1.4 144,931 + +0.2 144,931 + -1.3 144,932 - -0.4 144,932 - -0.7 144,932 - -1.1 144,932 - +0.3 144,932 - -0.2 145,259 + GFF5043 0.28 -0.0 145,259 + GFF5043 0.28 +0.0 145,259 + GFF5043 0.28 -0.9 145,259 + GFF5043 0.28 +0.8 145,259 + GFF5043 0.28 +0.9 145,259 + GFF5043 0.28 -1.5 145,259 + GFF5043 0.28 -0.5 145,259 + GFF5043 0.28 +1.0 145,259 + GFF5043 0.28 -2.5 145,260 - GFF5043 0.28 -1.2 145,260 - GFF5043 0.28 -1.7 145,260 - GFF5043 0.28 +1.1 145,260 - GFF5043 0.28 -0.3 145,260 - GFF5043 0.28 -0.6 145,260 - GFF5043 0.28 -1.9 145,451 + GFF5043 0.44 +0.2 145,452 - GFF5043 0.45 -0.4 145,452 - GFF5043 0.45 +0.1 145,452 - GFF5043 0.45 +1.7 145,497 + GFF5043 0.48 +0.1 145,498 - GFF5043 0.49 +0.3 145,498 - GFF5043 0.49 -1.5 145,520 + GFF5043 0.50 -0.2 145,521 - GFF5043 0.50 -0.2 145,521 - GFF5043 0.50 -0.9 145,521 - GFF5043 0.50 -1.2 145,521 - GFF5043 0.50 -2.1 145,716 + GFF5043 0.67 -1.1 145,716 + GFF5043 0.67 +1.7
Or see this region's nucleotide sequence