Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4803 and GFF4804 overlap by 4 nucleotides GFF4804 and GFF4805 are separated by 45 nucleotides GFF4805 and GFF4806 overlap by 4 nucleotides GFF4806 and GFF4807 are separated by 27 nucleotides
GFF4803 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 173,339 to 174,829
GFF4803
GFF4804 - tRNA threonylcarbamoyladenosine biosynthesis protein TsaE, at 174,826 to 175,383
GFF4804
GFF4805 - Epoxyqueuosine reductase (EC 1.17.99.6) QueG, at 175,429 to 176,391
GFF4805
GFF4806 - Arsenate reductase (EC 1.20.4.1) glutaredoxin-coupled, glutaredoxin-like family, at 176,388 to 176,789
GFF4806
GFF4807 - Uncharacterized GST-like protein yncG, at 176,817 to 177,503
GFF4807
Position (kb)
175
176
177 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 174.441 kb on + strand, within GFF4803 at 174.441 kb on + strand, within GFF4803 at 174.442 kb on - strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.554 kb on - strand, within GFF4803 at 174.702 kb on + strand at 174.702 kb on + strand at 174.702 kb on + strand at 174.702 kb on + strand at 174.703 kb on - strand at 174.703 kb on - strand at 174.703 kb on - strand at 174.703 kb on - strand at 175.306 kb on + strand, within GFF4804 at 175.306 kb on + strand, within GFF4804 at 175.306 kb on + strand, within GFF4804 at 175.348 kb on - strand at 175.348 kb on - strand at 175.407 kb on - strand at 175.407 kb on - strand at 175.407 kb on - strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.469 kb on - strand at 175.469 kb on - strand at 175.469 kb on - strand at 175.552 kb on + strand, within GFF4805 at 175.552 kb on + strand, within GFF4805 at 175.552 kb on + strand, within GFF4805 at 175.552 kb on + strand, within GFF4805 at 175.552 kb on + strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.636 kb on + strand, within GFF4805 at 175.636 kb on + strand, within GFF4805 at 175.636 kb on + strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.654 kb on + strand, within GFF4805 at 175.654 kb on + strand, within GFF4805 at 175.654 kb on + strand, within GFF4805 at 175.654 kb on + strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.859 kb on - strand, within GFF4805 at 175.859 kb on - strand, within GFF4805 at 175.988 kb on - strand, within GFF4805 at 175.990 kb on + strand, within GFF4805 at 175.991 kb on - strand, within GFF4805 at 176.056 kb on + strand, within GFF4805 at 176.056 kb on + strand, within GFF4805 at 176.056 kb on + strand, within GFF4805 at 176.056 kb on + strand, within GFF4805 at 176.056 kb on + strand, within GFF4805 at 176.057 kb on - strand, within GFF4805 at 176.057 kb on - strand, within GFF4805 at 176.057 kb on - strand, within GFF4805 at 176.058 kb on - strand, within GFF4805 at 176.389 kb on + strand at 176.389 kb on + strand at 176.389 kb on + strand at 176.390 kb on - strand at 176.390 kb on - strand at 176.390 kb on - strand at 176.390 kb on - strand at 176.390 kb on - strand at 176.569 kb on + strand, within GFF4806 at 176.569 kb on + strand, within GFF4806 at 176.570 kb on - strand, within GFF4806 at 176.570 kb on - strand, within GFF4806 at 176.665 kb on + strand, within GFF4806 at 176.746 kb on + strand, within GFF4806 at 176.746 kb on + strand, within GFF4806 at 176.747 kb on - strand, within GFF4806 at 176.747 kb on - strand, within GFF4806 at 176.747 kb on - strand, within GFF4806 at 176.800 kb on - strand at 176.818 kb on + strand at 176.819 kb on - strand at 176.819 kb on - strand at 176.819 kb on - strand at 176.819 kb on - strand at 176.935 kb on + strand, within GFF4807 at 176.980 kb on + strand, within GFF4807 at 176.980 kb on + strand, within GFF4807 at 176.980 kb on + strand, within GFF4807 at 176.981 kb on - strand, within GFF4807 at 176.981 kb on - strand, within GFF4807 at 176.981 kb on - strand, within GFF4807 at 176.981 kb on - strand, within GFF4807 at 177.016 kb on + strand, within GFF4807 at 177.157 kb on + strand, within GFF4807 at 177.157 kb on + strand, within GFF4807 at 177.157 kb on + strand, within GFF4807 at 177.157 kb on + strand, within GFF4807 at 177.157 kb on + strand, within GFF4807 at 177.158 kb on - strand, within GFF4807 at 177.158 kb on - strand, within GFF4807 at 177.175 kb on + strand, within GFF4807 at 177.175 kb on + strand, within GFF4807 at 177.194 kb on - strand, within GFF4807 at 177.309 kb on + strand, within GFF4807 at 177.309 kb on + strand, within GFF4807 at 177.310 kb on - strand, within GFF4807
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 174,441 + GFF4803 0.74 -0.1 174,441 + GFF4803 0.74 +2.3 174,442 - GFF4803 0.74 -1.1 174,552 + GFF4803 0.81 -0.8 174,552 + GFF4803 0.81 -1.2 174,552 + GFF4803 0.81 +2.3 174,552 + GFF4803 0.81 +0.0 174,552 + GFF4803 0.81 -0.3 174,552 + GFF4803 0.81 -0.4 174,553 - GFF4803 0.81 -1.1 174,553 - GFF4803 0.81 +0.4 174,553 - GFF4803 0.81 -0.9 174,553 - GFF4803 0.81 +0.2 174,553 - GFF4803 0.81 +0.1 174,553 - GFF4803 0.81 +0.1 174,553 - GFF4803 0.81 +1.1 174,554 - GFF4803 0.81 -0.6 174,702 + -1.1 174,702 + +0.0 174,702 + -0.2 174,702 + +0.2 174,703 - +1.4 174,703 - -1.3 174,703 - -1.8 174,703 - -0.3 175,306 + GFF4804 0.86 +0.2 175,306 + GFF4804 0.86 +0.8 175,306 + GFF4804 0.86 -2.2 175,348 - +1.2 175,348 - -1.2 175,407 - +0.3 175,407 - -2.1 175,407 - +0.5 175,468 + -0.8 175,468 + +0.0 175,468 + -1.3 175,468 + +0.5 175,468 + -0.2 175,468 + +1.2 175,468 + -0.3 175,468 + -1.1 175,468 + +0.4 175,468 + +0.5 175,468 + +0.3 175,468 + -0.7 175,468 + -0.2 175,468 + -0.3 175,468 + +0.2 175,469 - -0.3 175,469 - -0.3 175,469 - -0.5 175,552 + GFF4805 0.13 -1.7 175,552 + GFF4805 0.13 +1.2 175,552 + GFF4805 0.13 -0.1 175,552 + GFF4805 0.13 -0.6 175,552 + GFF4805 0.13 +0.5 175,553 - GFF4805 0.13 -0.5 175,553 - GFF4805 0.13 +0.7 175,553 - GFF4805 0.13 +1.4 175,553 - GFF4805 0.13 -1.5 175,553 - GFF4805 0.13 +0.7 175,553 - GFF4805 0.13 -0.5 175,636 + GFF4805 0.21 -0.7 175,636 + GFF4805 0.21 +1.2 175,636 + GFF4805 0.21 -0.2 175,637 - GFF4805 0.22 +0.5 175,637 - GFF4805 0.22 -1.1 175,637 - GFF4805 0.22 +0.3 175,637 - GFF4805 0.22 -0.4 175,637 - GFF4805 0.22 -2.0 175,637 - GFF4805 0.22 +0.2 175,637 - GFF4805 0.22 +0.2 175,637 - GFF4805 0.22 +0.4 175,637 - GFF4805 0.22 -0.6 175,637 - GFF4805 0.22 -0.5 175,637 - GFF4805 0.22 -1.4 175,637 - GFF4805 0.22 +2.5 175,637 - GFF4805 0.22 -2.8 175,654 + GFF4805 0.23 +0.1 175,654 + GFF4805 0.23 -0.5 175,654 + GFF4805 0.23 -0.7 175,654 + GFF4805 0.23 -1.2 175,655 - GFF4805 0.23 -0.0 175,655 - GFF4805 0.23 -2.3 175,655 - GFF4805 0.23 +0.8 175,655 - GFF4805 0.23 +1.9 175,655 - GFF4805 0.23 -2.1 175,655 - GFF4805 0.23 -1.2 175,858 + GFF4805 0.45 -0.5 175,858 + GFF4805 0.45 -1.7 175,858 + GFF4805 0.45 -1.1 175,858 + GFF4805 0.45 -0.2 175,858 + GFF4805 0.45 -0.7 175,858 + GFF4805 0.45 -1.9 175,858 + GFF4805 0.45 -1.1 175,859 - GFF4805 0.45 -0.2 175,859 - GFF4805 0.45 +0.0 175,988 - GFF4805 0.58 -0.7 175,990 + GFF4805 0.58 -3.4 175,991 - GFF4805 0.58 -0.1 176,056 + GFF4805 0.65 -0.4 176,056 + GFF4805 0.65 -1.1 176,056 + GFF4805 0.65 -0.4 176,056 + GFF4805 0.65 -1.3 176,056 + GFF4805 0.65 +0.5 176,057 - GFF4805 0.65 +0.4 176,057 - GFF4805 0.65 +0.5 176,057 - GFF4805 0.65 -0.2 176,058 - GFF4805 0.65 +0.5 176,389 + -0.3 176,389 + +2.8 176,389 + +0.6 176,390 - -0.9 176,390 - -0.9 176,390 - -0.6 176,390 - -1.7 176,390 - -2.7 176,569 + GFF4806 0.45 +0.7 176,569 + GFF4806 0.45 -0.8 176,570 - GFF4806 0.45 +0.4 176,570 - GFF4806 0.45 +0.3 176,665 + GFF4806 0.69 -1.5 176,746 + GFF4806 0.89 +0.9 176,746 + GFF4806 0.89 -0.4 176,747 - GFF4806 0.89 -0.5 176,747 - GFF4806 0.89 +0.2 176,747 - GFF4806 0.89 +0.3 176,800 - -0.3 176,818 + +0.6 176,819 - -1.4 176,819 - -1.4 176,819 - +1.2 176,819 - +0.7 176,935 + GFF4807 0.17 -0.6 176,980 + GFF4807 0.24 -0.1 176,980 + GFF4807 0.24 -1.0 176,980 + GFF4807 0.24 -0.9 176,981 - GFF4807 0.24 +0.2 176,981 - GFF4807 0.24 -0.5 176,981 - GFF4807 0.24 -0.5 176,981 - GFF4807 0.24 +1.3 177,016 + GFF4807 0.29 +0.3 177,157 + GFF4807 0.49 +0.7 177,157 + GFF4807 0.49 +0.4 177,157 + GFF4807 0.49 -0.3 177,157 + GFF4807 0.49 +0.3 177,157 + GFF4807 0.49 -2.7 177,158 - GFF4807 0.50 -0.7 177,158 - GFF4807 0.50 -0.2 177,175 + GFF4807 0.52 -1.9 177,175 + GFF4807 0.52 -0.9 177,194 - GFF4807 0.55 +0.1 177,309 + GFF4807 0.72 -1.0 177,309 + GFF4807 0.72 -0.6 177,310 - GFF4807 0.72 -0.3
Or see this region's nucleotide sequence