Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4732 and GFF4733 are separated by 9 nucleotides GFF4733 and GFF4734 overlap by 4 nucleotides GFF4734 and GFF4735 are separated by 24 nucleotides GFF4735 and GFF4736 overlap by 4 nucleotides
GFF4732 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 97,558 to 97,953
GFF4732
GFF4733 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 97,963 to 98,754
GFF4733
GFF4734 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 98,751 to 101,219
GFF4734
GFF4735 - Nitrate ABC transporter, ATP-binding protein, at 101,244 to 102,053
GFF4735
GFF4736 - Nitrate ABC transporter, permease protein, at 102,050 to 102,970
GFF4736
Position (kb)
98
99
100
101
102 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 97.964 kb on + strand at 97.964 kb on + strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.982 kb on + strand at 97.983 kb on - strand at 98.258 kb on + strand, within GFF4733 at 98.259 kb on - strand, within GFF4733 at 98.345 kb on + strand, within GFF4733 at 98.480 kb on + strand, within GFF4733 at 98.481 kb on - strand, within GFF4733 at 98.609 kb on + strand, within GFF4733 at 98.609 kb on + strand, within GFF4733 at 98.610 kb on - strand, within GFF4733 at 98.610 kb on - strand, within GFF4733 at 98.921 kb on - strand at 98.944 kb on + strand at 98.944 kb on + strand at 98.944 kb on + strand at 98.945 kb on - strand at 98.945 kb on - strand at 98.965 kb on + strand at 98.966 kb on - strand at 99.007 kb on + strand, within GFF4734 at 99.008 kb on - strand, within GFF4734 at 99.008 kb on - strand, within GFF4734 at 99.084 kb on + strand, within GFF4734 at 99.084 kb on + strand, within GFF4734 at 99.085 kb on - strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.089 kb on - strand, within GFF4734 at 99.089 kb on - strand, within GFF4734 at 99.196 kb on + strand, within GFF4734 at 99.196 kb on + strand, within GFF4734 at 99.197 kb on - strand, within GFF4734 at 99.311 kb on - strand, within GFF4734 at 99.424 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.503 kb on - strand, within GFF4734 at 99.503 kb on - strand, within GFF4734 at 99.598 kb on + strand, within GFF4734 at 99.598 kb on + strand, within GFF4734 at 99.599 kb on - strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.602 kb on - strand, within GFF4734 at 99.602 kb on - strand, within GFF4734 at 99.682 kb on + strand, within GFF4734 at 99.682 kb on + strand, within GFF4734 at 99.769 kb on + strand, within GFF4734 at 99.770 kb on - strand, within GFF4734 at 100.067 kb on - strand, within GFF4734 at 100.067 kb on - strand, within GFF4734 at 100.141 kb on + strand, within GFF4734 at 100.142 kb on - strand, within GFF4734 at 100.264 kb on + strand, within GFF4734 at 100.264 kb on + strand, within GFF4734 at 100.264 kb on + strand, within GFF4734 at 100.265 kb on - strand, within GFF4734 at 100.378 kb on + strand, within GFF4734 at 100.378 kb on + strand, within GFF4734 at 100.379 kb on - strand, within GFF4734 at 100.901 kb on - strand, within GFF4734 at 101.077 kb on + strand at 101.077 kb on + strand at 101.077 kb on + strand at 101.078 kb on - strand at 101.078 kb on - strand at 101.116 kb on + strand at 101.116 kb on + strand at 101.116 kb on + strand at 101.281 kb on + strand at 101.281 kb on + strand at 101.282 kb on - strand at 101.317 kb on + strand at 101.317 kb on + strand at 101.317 kb on + strand at 101.317 kb on + strand at 101.318 kb on - strand at 101.749 kb on + strand, within GFF4735 at 101.749 kb on + strand, within GFF4735 at 101.749 kb on + strand, within GFF4735 at 101.749 kb on + strand, within GFF4735 at 101.750 kb on - strand, within GFF4735 at 101.750 kb on - strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.751 kb on + strand, within GFF4735 at 101.752 kb on - strand, within GFF4735 at 101.752 kb on - strand, within GFF4735 at 101.752 kb on - strand, within GFF4735 at 101.752 kb on - strand, within GFF4735 at 102.034 kb on + strand at 102.034 kb on + strand at 102.034 kb on + strand at 102.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 97,964 + -0.1 97,964 + -2.4 97,965 - -0.5 97,965 - +0.5 97,965 - -0.1 97,965 - +0.3 97,982 + +0.1 97,983 - +0.9 98,258 + GFF4733 0.37 -0.9 98,259 - GFF4733 0.37 -0.8 98,345 + GFF4733 0.48 -1.1 98,480 + GFF4733 0.65 -2.7 98,481 - GFF4733 0.65 +1.8 98,609 + GFF4733 0.82 +3.1 98,609 + GFF4733 0.82 -0.7 98,610 - GFF4733 0.82 -0.2 98,610 - GFF4733 0.82 +3.1 98,921 - -1.3 98,944 + +0.2 98,944 + +0.1 98,944 + -0.6 98,945 - +1.9 98,945 - +2.2 98,965 + -0.6 98,966 - -0.5 99,007 + GFF4734 0.10 -0.1 99,008 - GFF4734 0.10 -0.8 99,008 - GFF4734 0.10 -0.1 99,084 + GFF4734 0.13 -0.6 99,084 + GFF4734 0.13 -0.5 99,085 - GFF4734 0.14 -0.1 99,088 + GFF4734 0.14 -2.1 99,088 + GFF4734 0.14 -0.4 99,088 + GFF4734 0.14 +0.5 99,088 + GFF4734 0.14 -0.1 99,088 + GFF4734 0.14 -0.2 99,088 + GFF4734 0.14 -0.4 99,089 - GFF4734 0.14 -0.1 99,089 - GFF4734 0.14 -0.6 99,196 + GFF4734 0.18 -0.1 99,196 + GFF4734 0.18 +0.7 99,197 - GFF4734 0.18 -0.6 99,311 - GFF4734 0.23 -0.2 99,424 + GFF4734 0.27 -0.7 99,502 + GFF4734 0.30 -0.3 99,502 + GFF4734 0.30 +0.3 99,502 + GFF4734 0.30 -0.3 99,502 + GFF4734 0.30 -0.4 99,503 - GFF4734 0.30 -1.1 99,503 - GFF4734 0.30 +1.5 99,598 + GFF4734 0.34 -0.2 99,598 + GFF4734 0.34 +0.2 99,599 - GFF4734 0.34 -0.2 99,601 + GFF4734 0.34 -0.1 99,601 + GFF4734 0.34 +0.6 99,601 + GFF4734 0.34 +0.5 99,601 + GFF4734 0.34 -0.1 99,601 + GFF4734 0.34 -0.7 99,601 + GFF4734 0.34 -2.5 99,601 + GFF4734 0.34 -1.3 99,602 - GFF4734 0.34 -0.7 99,602 - GFF4734 0.34 +0.0 99,682 + GFF4734 0.38 +1.4 99,682 + GFF4734 0.38 -0.3 99,769 + GFF4734 0.41 -0.8 99,770 - GFF4734 0.41 -0.5 100,067 - GFF4734 0.53 +0.9 100,067 - GFF4734 0.53 +0.3 100,141 + GFF4734 0.56 +0.7 100,142 - GFF4734 0.56 -0.4 100,264 + GFF4734 0.61 -1.9 100,264 + GFF4734 0.61 +0.1 100,264 + GFF4734 0.61 +0.3 100,265 - GFF4734 0.61 +0.8 100,378 + GFF4734 0.66 -0.5 100,378 + GFF4734 0.66 -0.1 100,379 - GFF4734 0.66 +1.5 100,901 - GFF4734 0.87 -0.3 101,077 + -0.1 101,077 + +1.4 101,077 + -0.2 101,078 - -0.8 101,078 - -0.5 101,116 + +2.1 101,116 + -0.1 101,116 + -0.1 101,281 + -0.7 101,281 + +0.3 101,282 - +0.5 101,317 + +0.8 101,317 + +1.0 101,317 + -1.1 101,317 + +0.2 101,318 - +0.3 101,749 + GFF4735 0.62 +0.2 101,749 + GFF4735 0.62 -1.0 101,749 + GFF4735 0.62 +0.1 101,749 + GFF4735 0.62 -1.7 101,750 - GFF4735 0.62 -0.1 101,750 - GFF4735 0.62 -1.3 101,751 + GFF4735 0.63 +0.2 101,751 + GFF4735 0.63 +0.1 101,751 + GFF4735 0.63 +0.2 101,751 + GFF4735 0.63 +0.9 101,751 + GFF4735 0.63 +1.7 101,751 + GFF4735 0.63 +0.9 101,751 + GFF4735 0.63 +0.8 101,751 + GFF4735 0.63 +0.9 101,752 - GFF4735 0.63 -1.1 101,752 - GFF4735 0.63 -0.2 101,752 - GFF4735 0.63 +0.5 101,752 - GFF4735 0.63 -0.7 102,034 + -1.2 102,034 + +0.8 102,034 + -0.1 102,035 - -0.5
Or see this region's nucleotide sequence