Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF462 and GFF463 are separated by 100 nucleotides GFF463 and GFF464 are separated by 28 nucleotides GFF464 and GFF465 are separated by 112 nucleotides
GFF462 - BUG/TctC family periplasmic protein, at 500,952 to 501,938
GFF462
GFF463 - Malonyl-CoA decarboxylase (EC 4.1.1.9), at 502,039 to 503,505
GFF463
GFF464 - Transcriptional regulator, GntR family, at 503,534 to 504,202
GFF464
GFF465 - Prolipoprotein diacylglyceryl transferase, at 504,315 to 505,139
GFF465
Position (kb)
502
503
504 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 501.170 kb on - strand, within GFF462 at 501.187 kb on + strand, within GFF462 at 501.187 kb on + strand, within GFF462 at 501.188 kb on - strand, within GFF462 at 501.188 kb on - strand, within GFF462 at 501.188 kb on - strand, within GFF462 at 501.219 kb on + strand, within GFF462 at 501.219 kb on + strand, within GFF462 at 501.219 kb on + strand, within GFF462 at 501.219 kb on + strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.220 kb on - strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.289 kb on + strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.290 kb on - strand, within GFF462 at 501.460 kb on + strand, within GFF462 at 501.460 kb on + strand, within GFF462 at 501.601 kb on + strand, within GFF462 at 501.665 kb on - strand, within GFF462 at 502.136 kb on + strand at 502.137 kb on - strand at 502.137 kb on - strand at 502.142 kb on + strand at 502.142 kb on + strand at 502.142 kb on + strand at 502.143 kb on - strand at 502.143 kb on - strand at 502.143 kb on - strand at 502.143 kb on - strand at 502.161 kb on - strand at 502.289 kb on + strand, within GFF463 at 502.290 kb on - strand, within GFF463 at 502.763 kb on + strand, within GFF463 at 502.763 kb on + strand, within GFF463 at 502.764 kb on - strand, within GFF463 at 502.952 kb on - strand, within GFF463 at 503.196 kb on - strand, within GFF463 at 503.229 kb on - strand, within GFF463 at 503.732 kb on + strand, within GFF464 at 503.732 kb on + strand, within GFF464 at 503.733 kb on - strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.824 kb on - strand, within GFF464 at 503.824 kb on - strand, within GFF464 at 503.824 kb on - strand, within GFF464 at 503.824 kb on - strand, within GFF464 at 503.824 kb on - strand, within GFF464 at 503.923 kb on - strand, within GFF464 at 503.923 kb on - strand, within GFF464 at 504.230 kb on + strand at 504.237 kb on - strand at 504.296 kb on + strand at 504.296 kb on + strand at 504.296 kb on + strand at 504.296 kb on + strand at 504.296 kb on + strand at 504.296 kb on + strand at 504.297 kb on - strand at 504.297 kb on - strand at 504.297 kb on - strand at 504.297 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 501,170 - GFF462 0.22 -2.1 501,187 + GFF462 0.24 -1.3 501,187 + GFF462 0.24 +2.4 501,188 - GFF462 0.24 -0.0 501,188 - GFF462 0.24 -0.7 501,188 - GFF462 0.24 -0.6 501,219 + GFF462 0.27 -1.0 501,219 + GFF462 0.27 -0.6 501,219 + GFF462 0.27 -0.9 501,219 + GFF462 0.27 -0.5 501,220 - GFF462 0.27 -0.1 501,220 - GFF462 0.27 -1.8 501,220 - GFF462 0.27 -2.3 501,220 - GFF462 0.27 -1.1 501,220 - GFF462 0.27 -1.1 501,220 - GFF462 0.27 +0.9 501,220 - GFF462 0.27 -0.5 501,220 - GFF462 0.27 +1.0 501,289 + GFF462 0.34 -0.6 501,289 + GFF462 0.34 -0.4 501,289 + GFF462 0.34 -1.3 501,289 + GFF462 0.34 -1.9 501,289 + GFF462 0.34 -0.0 501,289 + GFF462 0.34 -0.9 501,289 + GFF462 0.34 +1.4 501,289 + GFF462 0.34 -0.5 501,290 - GFF462 0.34 -0.5 501,290 - GFF462 0.34 -0.0 501,290 - GFF462 0.34 +1.7 501,290 - GFF462 0.34 +0.7 501,290 - GFF462 0.34 -0.9 501,290 - GFF462 0.34 -0.8 501,290 - GFF462 0.34 -1.2 501,460 + GFF462 0.51 -0.3 501,460 + GFF462 0.51 +0.4 501,601 + GFF462 0.66 +1.0 501,665 - GFF462 0.72 +0.1 502,136 + +0.4 502,137 - -0.3 502,137 - +1.4 502,142 + -0.1 502,142 + -1.5 502,142 + -1.2 502,143 - +1.6 502,143 - -0.1 502,143 - -0.8 502,143 - -0.4 502,161 - -0.8 502,289 + GFF463 0.17 -2.5 502,290 - GFF463 0.17 -1.1 502,763 + GFF463 0.49 -0.4 502,763 + GFF463 0.49 -1.6 502,764 - GFF463 0.49 -0.2 502,952 - GFF463 0.62 -0.8 503,196 - GFF463 0.79 -1.6 503,229 - GFF463 0.81 -0.5 503,732 + GFF464 0.30 -0.6 503,732 + GFF464 0.30 -0.7 503,733 - GFF464 0.30 +0.5 503,820 + GFF464 0.43 +0.0 503,820 + GFF464 0.43 +1.2 503,820 + GFF464 0.43 -1.8 503,820 + GFF464 0.43 +0.0 503,820 + GFF464 0.43 -0.3 503,820 + GFF464 0.43 +0.4 503,821 - GFF464 0.43 +0.3 503,821 - GFF464 0.43 -0.8 503,821 - GFF464 0.43 +0.3 503,821 - GFF464 0.43 +0.2 503,821 - GFF464 0.43 +0.1 503,821 - GFF464 0.43 -1.4 503,821 - GFF464 0.43 -0.1 503,823 + GFF464 0.43 -0.1 503,823 + GFF464 0.43 +0.8 503,823 + GFF464 0.43 -0.7 503,823 + GFF464 0.43 -1.0 503,823 + GFF464 0.43 -0.9 503,823 + GFF464 0.43 +0.3 503,824 - GFF464 0.43 -0.9 503,824 - GFF464 0.43 -0.5 503,824 - GFF464 0.43 -0.2 503,824 - GFF464 0.43 -0.4 503,824 - GFF464 0.43 -0.7 503,923 - GFF464 0.58 -0.4 503,923 - GFF464 0.58 +0.1 504,230 + -0.3 504,237 - +1.0 504,296 + +1.0 504,296 + -1.5 504,296 + +0.1 504,296 + -0.2 504,296 + -1.0 504,296 + +0.1 504,297 - -0.2 504,297 - +0.7 504,297 - +1.8 504,297 - +0.6
Or see this region's nucleotide sequence