Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4603 and GFF4604 are separated by 2 nucleotides GFF4604 and GFF4605 are separated by 29 nucleotides GFF4605 and GFF4606 are separated by 55 nucleotides
GFF4603 - Short-chain dehydrogenase, associated with 2-hydroxychromene-2-carboxylate isomerase family protein, at 244,786 to 245,535
GFF4603
GFF4604 - TesB-like acyl-CoA thioesterase 2, at 245,538 to 246,374
GFF4604
GFF4605 - FIG00537210: hypothetical protein, at 246,404 to 247,309
GFF4605
GFF4606 - 3-ketoacyl-CoA thiolase [fadN-fadA-fadE operon] (EC 2.3.1.16), at 247,365 to 248,561
GFF4606
Position (kb) 246
247
248 Strain fitness (log2 ratio) -2
-1
0
1 at 245.530 kb on - strand at 245.530 kb on - strand at 245.530 kb on - strand at 245.548 kb on + strand at 245.549 kb on - strand at 245.549 kb on - strand at 245.549 kb on - strand at 245.551 kb on + strand at 245.552 kb on - strand at 245.552 kb on - strand at 245.662 kb on + strand, within GFF4604 at 245.662 kb on + strand, within GFF4604 at 245.662 kb on + strand, within GFF4604 at 245.662 kb on + strand, within GFF4604 at 245.663 kb on - strand, within GFF4604 at 245.663 kb on - strand, within GFF4604 at 245.707 kb on + strand, within GFF4604 at 245.707 kb on + strand, within GFF4604 at 245.707 kb on + strand, within GFF4604 at 245.708 kb on - strand, within GFF4604 at 245.708 kb on - strand, within GFF4604 at 245.708 kb on - strand, within GFF4604 at 245.770 kb on + strand, within GFF4604 at 245.911 kb on + strand, within GFF4604 at 246.172 kb on + strand, within GFF4604 at 246.172 kb on + strand, within GFF4604 at 246.172 kb on + strand, within GFF4604 at 246.281 kb on - strand, within GFF4604 at 246.591 kb on + strand, within GFF4605 at 246.591 kb on + strand, within GFF4605 at 246.592 kb on - strand, within GFF4605 at 246.592 kb on - strand, within GFF4605 at 246.681 kb on + strand, within GFF4605 at 246.682 kb on - strand, within GFF4605 at 246.682 kb on - strand, within GFF4605 at 246.807 kb on + strand, within GFF4605 at 246.853 kb on - strand, within GFF4605 at 246.853 kb on - strand, within GFF4605 at 246.991 kb on - strand, within GFF4605 at 246.991 kb on - strand, within GFF4605 at 247.179 kb on + strand, within GFF4605 at 247.179 kb on + strand, within GFF4605 at 247.180 kb on - strand, within GFF4605 at 247.180 kb on - strand, within GFF4605 at 247.248 kb on + strand at 247.249 kb on - strand at 247.429 kb on + strand at 247.430 kb on - strand at 247.640 kb on - strand, within GFF4606
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 245,530 - -1.8 245,530 - +0.4 245,530 - +0.4 245,548 + -0.8 245,549 - -0.3 245,549 - -0.3 245,549 - -0.6 245,551 + +0.1 245,552 - -1.4 245,552 - -0.9 245,662 + GFF4604 0.15 -0.6 245,662 + GFF4604 0.15 -0.1 245,662 + GFF4604 0.15 +0.1 245,662 + GFF4604 0.15 -0.5 245,663 - GFF4604 0.15 -0.3 245,663 - GFF4604 0.15 -0.5 245,707 + GFF4604 0.20 +0.1 245,707 + GFF4604 0.20 -0.8 245,707 + GFF4604 0.20 +0.4 245,708 - GFF4604 0.20 -0.2 245,708 - GFF4604 0.20 -1.5 245,708 - GFF4604 0.20 +0.2 245,770 + GFF4604 0.28 -0.4 245,911 + GFF4604 0.45 -0.2 246,172 + GFF4604 0.76 -0.7 246,172 + GFF4604 0.76 -2.4 246,172 + GFF4604 0.76 +0.6 246,281 - GFF4604 0.89 -1.9 246,591 + GFF4605 0.21 -0.1 246,591 + GFF4605 0.21 -0.7 246,592 - GFF4605 0.21 -0.2 246,592 - GFF4605 0.21 +0.2 246,681 + GFF4605 0.31 -2.1 246,682 - GFF4605 0.31 -0.5 246,682 - GFF4605 0.31 -0.4 246,807 + GFF4605 0.44 -1.5 246,853 - GFF4605 0.50 +0.5 246,853 - GFF4605 0.50 +0.3 246,991 - GFF4605 0.65 -0.5 246,991 - GFF4605 0.65 -0.4 247,179 + GFF4605 0.86 -0.6 247,179 + GFF4605 0.86 +0.0 247,180 - GFF4605 0.86 -0.5 247,180 - GFF4605 0.86 -0.4 247,248 + -0.5 247,249 - +0.8 247,429 + +0.0 247,430 - -0.2 247,640 - GFF4606 0.23 -1.1
Or see this region's nucleotide sequence