Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF459 and GFF460 overlap by 4 nucleotides GFF460 and GFF461 are separated by 15 nucleotides GFF461 and GFF462 overlap by 4 nucleotides
GFF459 - Beta-lactamase class C-like and penicillin binding proteins (PBPs) superfamily / DUF3471 domain, at 497,159 to 498,604
GFF459
GFF460 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 498,601 to 500,139
GFF460
GFF461 - Enoyl-CoA hydratase (EC 4.2.1.17), at 500,155 to 500,955
GFF461
GFF462 - BUG/TctC family periplasmic protein, at 500,952 to 501,938
GFF462
Position (kb)
498
499
500
501 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 497.618 kb on + strand, within GFF459 at 497.618 kb on + strand, within GFF459 at 497.618 kb on + strand, within GFF459 at 497.618 kb on + strand, within GFF459 at 497.618 kb on + strand, within GFF459 at 497.619 kb on - strand, within GFF459 at 497.619 kb on - strand, within GFF459 at 498.041 kb on + strand, within GFF459 at 498.041 kb on + strand, within GFF459 at 498.042 kb on - strand, within GFF459 at 498.161 kb on + strand, within GFF459 at 498.161 kb on + strand, within GFF459 at 498.162 kb on - strand, within GFF459 at 498.320 kb on + strand, within GFF459 at 498.320 kb on - strand, within GFF459 at 498.321 kb on - strand, within GFF459 at 498.321 kb on - strand, within GFF459 at 498.333 kb on - strand, within GFF459 at 498.333 kb on - strand, within GFF459 at 498.333 kb on - strand, within GFF459 at 498.348 kb on - strand, within GFF459 at 498.431 kb on + strand, within GFF459 at 498.431 kb on + strand, within GFF459 at 498.431 kb on + strand, within GFF459 at 498.501 kb on - strand at 498.866 kb on + strand, within GFF460 at 498.867 kb on - strand, within GFF460 at 498.887 kb on + strand, within GFF460 at 498.896 kb on + strand, within GFF460 at 498.896 kb on + strand, within GFF460 at 498.897 kb on - strand, within GFF460 at 498.897 kb on - strand, within GFF460 at 498.897 kb on - strand, within GFF460 at 498.897 kb on - strand, within GFF460 at 498.897 kb on - strand, within GFF460 at 498.944 kb on + strand, within GFF460 at 498.944 kb on + strand, within GFF460 at 498.944 kb on + strand, within GFF460 at 498.944 kb on + strand, within GFF460 at 499.034 kb on + strand, within GFF460 at 499.034 kb on + strand, within GFF460 at 499.035 kb on - strand, within GFF460 at 499.184 kb on + strand, within GFF460 at 499.184 kb on + strand, within GFF460 at 499.184 kb on + strand, within GFF460 at 499.185 kb on - strand, within GFF460 at 499.185 kb on - strand, within GFF460 at 499.185 kb on - strand, within GFF460 at 499.185 kb on - strand, within GFF460 at 499.185 kb on - strand, within GFF460 at 499.364 kb on + strand, within GFF460 at 499.364 kb on + strand, within GFF460 at 499.364 kb on + strand, within GFF460 at 499.364 kb on + strand, within GFF460 at 499.365 kb on - strand, within GFF460 at 499.365 kb on - strand, within GFF460 at 499.365 kb on - strand, within GFF460 at 499.365 kb on - strand, within GFF460 at 499.365 kb on - strand, within GFF460 at 499.553 kb on + strand, within GFF460 at 499.553 kb on + strand, within GFF460 at 499.554 kb on - strand, within GFF460 at 499.554 kb on - strand, within GFF460 at 499.637 kb on + strand, within GFF460 at 499.638 kb on - strand, within GFF460 at 499.806 kb on + strand, within GFF460 at 499.806 kb on + strand, within GFF460 at 499.806 kb on + strand, within GFF460 at 499.807 kb on - strand, within GFF460 at 499.807 kb on - strand, within GFF460 at 499.841 kb on + strand, within GFF460 at 499.845 kb on - strand, within GFF460 at 499.892 kb on + strand, within GFF460 at 499.916 kb on + strand, within GFF460 at 499.916 kb on + strand, within GFF460 at 499.916 kb on + strand, within GFF460 at 499.917 kb on - strand, within GFF460 at 499.917 kb on - strand, within GFF460 at 500.039 kb on + strand at 500.040 kb on - strand at 500.148 kb on + strand at 500.148 kb on + strand at 500.148 kb on + strand at 500.149 kb on - strand at 500.149 kb on - strand at 500.151 kb on + strand at 500.151 kb on + strand at 500.151 kb on + strand at 500.151 kb on + strand at 500.151 kb on + strand at 500.151 kb on + strand at 500.152 kb on - strand at 500.152 kb on - strand at 500.152 kb on - strand at 500.152 kb on - strand at 500.152 kb on - strand at 500.222 kb on + strand at 500.222 kb on + strand at 500.222 kb on + strand at 500.222 kb on + strand at 500.223 kb on - strand at 500.223 kb on - strand at 500.223 kb on - strand at 500.223 kb on - strand at 500.223 kb on - strand at 500.223 kb on - strand at 500.228 kb on + strand at 500.228 kb on + strand at 500.229 kb on - strand at 500.402 kb on + strand, within GFF461 at 500.402 kb on + strand, within GFF461 at 500.523 kb on + strand, within GFF461 at 500.524 kb on - strand, within GFF461
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 497,618 + GFF459 0.32 -0.9 497,618 + GFF459 0.32 -1.7 497,618 + GFF459 0.32 -0.2 497,618 + GFF459 0.32 -0.3 497,618 + GFF459 0.32 -0.5 497,619 - GFF459 0.32 -0.8 497,619 - GFF459 0.32 +0.9 498,041 + GFF459 0.61 +0.5 498,041 + GFF459 0.61 +1.0 498,042 - GFF459 0.61 +0.0 498,161 + GFF459 0.69 +0.4 498,161 + GFF459 0.69 -1.3 498,162 - GFF459 0.69 -0.6 498,320 + GFF459 0.80 -0.8 498,320 - GFF459 0.80 -0.7 498,321 - GFF459 0.80 -0.5 498,321 - GFF459 0.80 +0.4 498,333 - GFF459 0.81 +0.1 498,333 - GFF459 0.81 +1.4 498,333 - GFF459 0.81 -1.7 498,348 - GFF459 0.82 +1.0 498,431 + GFF459 0.88 -0.1 498,431 + GFF459 0.88 -0.8 498,431 + GFF459 0.88 -0.3 498,501 - +0.4 498,866 + GFF460 0.17 -0.9 498,867 - GFF460 0.17 -0.6 498,887 + GFF460 0.19 -0.4 498,896 + GFF460 0.19 -1.0 498,896 + GFF460 0.19 -1.6 498,897 - GFF460 0.19 -0.0 498,897 - GFF460 0.19 -0.6 498,897 - GFF460 0.19 -0.0 498,897 - GFF460 0.19 -0.8 498,897 - GFF460 0.19 +0.4 498,944 + GFF460 0.22 -0.6 498,944 + GFF460 0.22 -1.2 498,944 + GFF460 0.22 +0.4 498,944 + GFF460 0.22 -0.9 499,034 + GFF460 0.28 -0.9 499,034 + GFF460 0.28 -0.9 499,035 - GFF460 0.28 -0.7 499,184 + GFF460 0.38 -0.3 499,184 + GFF460 0.38 -0.0 499,184 + GFF460 0.38 -0.4 499,185 - GFF460 0.38 -0.8 499,185 - GFF460 0.38 -1.3 499,185 - GFF460 0.38 -0.9 499,185 - GFF460 0.38 -0.9 499,185 - GFF460 0.38 +0.9 499,364 + GFF460 0.50 -1.4 499,364 + GFF460 0.50 +0.3 499,364 + GFF460 0.50 +0.4 499,364 + GFF460 0.50 -1.3 499,365 - GFF460 0.50 -0.5 499,365 - GFF460 0.50 -0.5 499,365 - GFF460 0.50 -0.2 499,365 - GFF460 0.50 +0.1 499,365 - GFF460 0.50 -1.0 499,553 + GFF460 0.62 +0.4 499,553 + GFF460 0.62 +0.3 499,554 - GFF460 0.62 -0.8 499,554 - GFF460 0.62 -2.3 499,637 + GFF460 0.67 +0.8 499,638 - GFF460 0.67 -0.2 499,806 + GFF460 0.78 -0.5 499,806 + GFF460 0.78 -1.4 499,806 + GFF460 0.78 +0.2 499,807 - GFF460 0.78 -0.3 499,807 - GFF460 0.78 +0.4 499,841 + GFF460 0.81 -0.1 499,845 - GFF460 0.81 -0.9 499,892 + GFF460 0.84 +0.9 499,916 + GFF460 0.85 +0.6 499,916 + GFF460 0.85 +0.0 499,916 + GFF460 0.85 -1.8 499,917 - GFF460 0.86 -0.0 499,917 - GFF460 0.86 -3.0 500,039 + +0.5 500,040 - -1.2 500,148 + -0.0 500,148 + +0.8 500,148 + -0.0 500,149 - +0.9 500,149 - +0.2 500,151 + +1.0 500,151 + -0.8 500,151 + +0.8 500,151 + -0.6 500,151 + +1.0 500,151 + -0.4 500,152 - -1.6 500,152 - +0.6 500,152 - +0.0 500,152 - -0.6 500,152 - +0.4 500,222 + +0.9 500,222 + +0.1 500,222 + -0.6 500,222 + -0.6 500,223 - +0.9 500,223 - +1.9 500,223 - -0.1 500,223 - -0.3 500,223 - -0.1 500,223 - -1.2 500,228 + -0.5 500,228 + +0.3 500,229 - +0.4 500,402 + GFF461 0.31 -0.5 500,402 + GFF461 0.31 -0.3 500,523 + GFF461 0.46 -1.6 500,524 - GFF461 0.46 +0.2
Or see this region's nucleotide sequence