Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4568 and GFF4569 are separated by 31 nucleotides GFF4569 and GFF4570 are separated by 10 nucleotides
GFF4568 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 204,519 to 206,654
GFF4568
GFF4569 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 206,686 to 207,876
GFF4569
GFF4570 - Acyl-CoA dehydrogenase, long-chain specific (EC 1.3.8.8), at 207,887 to 209,095
GFF4570
Position (kb)
206
207
208 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 205.782 kb on + strand, within GFF4568 at 205.782 kb on + strand, within GFF4568 at 205.783 kb on - strand, within GFF4568 at 205.783 kb on - strand, within GFF4568 at 205.783 kb on - strand, within GFF4568 at 205.783 kb on - strand, within GFF4568 at 205.783 kb on - strand, within GFF4568 at 206.055 kb on + strand, within GFF4568 at 206.056 kb on - strand, within GFF4568 at 206.188 kb on - strand, within GFF4568 at 206.250 kb on + strand, within GFF4568 at 206.251 kb on - strand, within GFF4568 at 206.253 kb on + strand, within GFF4568 at 206.253 kb on + strand, within GFF4568 at 206.253 kb on + strand, within GFF4568 at 206.253 kb on + strand, within GFF4568 at 206.253 kb on + strand, within GFF4568 at 206.254 kb on - strand, within GFF4568 at 206.254 kb on - strand, within GFF4568 at 206.254 kb on - strand, within GFF4568 at 206.254 kb on - strand, within GFF4568 at 206.254 kb on - strand, within GFF4568 at 206.379 kb on + strand, within GFF4568 at 206.379 kb on + strand, within GFF4568 at 206.380 kb on - strand, within GFF4568 at 206.380 kb on - strand, within GFF4568 at 206.466 kb on + strand at 206.466 kb on + strand at 206.467 kb on - strand at 206.467 kb on - strand at 206.472 kb on + strand at 206.508 kb on + strand at 206.508 kb on + strand at 206.509 kb on - strand at 206.509 kb on - strand at 206.652 kb on + strand at 206.653 kb on - strand at 206.653 kb on - strand at 207.127 kb on + strand, within GFF4569 at 207.127 kb on + strand, within GFF4569 at 207.127 kb on + strand, within GFF4569 at 207.127 kb on + strand, within GFF4569 at 207.127 kb on + strand, within GFF4569 at 207.127 kb on + strand, within GFF4569 at 207.127 kb on + strand, within GFF4569 at 207.127 kb on + strand, within GFF4569 at 207.127 kb on + strand, within GFF4569 at 207.127 kb on + strand, within GFF4569 at 207.127 kb on + strand, within GFF4569 at 207.128 kb on - strand, within GFF4569 at 207.128 kb on - strand, within GFF4569 at 207.128 kb on - strand, within GFF4569 at 207.128 kb on - strand, within GFF4569 at 207.128 kb on - strand, within GFF4569 at 207.169 kb on + strand, within GFF4569 at 207.170 kb on - strand, within GFF4569 at 207.170 kb on - strand, within GFF4569 at 207.250 kb on + strand, within GFF4569 at 207.251 kb on - strand, within GFF4569 at 207.370 kb on + strand, within GFF4569 at 207.371 kb on - strand, within GFF4569 at 207.371 kb on - strand, within GFF4569 at 207.371 kb on - strand, within GFF4569 at 207.371 kb on - strand, within GFF4569 at 207.371 kb on - strand, within GFF4569 at 207.371 kb on - strand, within GFF4569 at 207.670 kb on + strand, within GFF4569 at 207.670 kb on + strand, within GFF4569 at 207.670 kb on + strand, within GFF4569 at 207.671 kb on - strand, within GFF4569 at 207.811 kb on + strand at 207.811 kb on + strand at 207.812 kb on - strand at 207.812 kb on - strand at 207.871 kb on + strand at 207.872 kb on - strand at 208.196 kb on + strand, within GFF4570 at 208.196 kb on + strand, within GFF4570 at 208.197 kb on - strand, within GFF4570 at 208.269 kb on - strand, within GFF4570 at 208.349 kb on + strand, within GFF4570 at 208.349 kb on + strand, within GFF4570 at 208.349 kb on + strand, within GFF4570 at 208.350 kb on - strand, within GFF4570 at 208.350 kb on - strand, within GFF4570 at 208.350 kb on - strand, within GFF4570 at 208.350 kb on - strand, within GFF4570 at 208.350 kb on - strand, within GFF4570 at 208.350 kb on - strand, within GFF4570 at 208.350 kb on - strand, within GFF4570 at 208.350 kb on - strand, within GFF4570 at 208.507 kb on + strand, within GFF4570 at 208.507 kb on + strand, within GFF4570 at 208.508 kb on - strand, within GFF4570 at 208.613 kb on + strand, within GFF4570 at 208.614 kb on - strand, within GFF4570 at 208.614 kb on - strand, within GFF4570 at 208.614 kb on - strand, within GFF4570 at 208.625 kb on + strand, within GFF4570 at 208.626 kb on - strand, within GFF4570 at 208.626 kb on - strand, within GFF4570 at 208.626 kb on - strand, within GFF4570
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 205,782 + GFF4568 0.59 +0.4 205,782 + GFF4568 0.59 +0.1 205,783 - GFF4568 0.59 -0.6 205,783 - GFF4568 0.59 -0.7 205,783 - GFF4568 0.59 -0.4 205,783 - GFF4568 0.59 +0.4 205,783 - GFF4568 0.59 +0.4 206,055 + GFF4568 0.72 -0.5 206,056 - GFF4568 0.72 +0.0 206,188 - GFF4568 0.78 -0.2 206,250 + GFF4568 0.81 +0.1 206,251 - GFF4568 0.81 +0.1 206,253 + GFF4568 0.81 -0.2 206,253 + GFF4568 0.81 +0.8 206,253 + GFF4568 0.81 +0.1 206,253 + GFF4568 0.81 -0.5 206,253 + GFF4568 0.81 +0.1 206,254 - GFF4568 0.81 -0.8 206,254 - GFF4568 0.81 +0.9 206,254 - GFF4568 0.81 -0.7 206,254 - GFF4568 0.81 -1.8 206,254 - GFF4568 0.81 -0.2 206,379 + GFF4568 0.87 +0.0 206,379 + GFF4568 0.87 +1.4 206,380 - GFF4568 0.87 +0.7 206,380 - GFF4568 0.87 -0.5 206,466 + -0.2 206,466 + +0.6 206,467 - -0.3 206,467 - +0.8 206,472 + -0.8 206,508 + -0.5 206,508 + -0.7 206,509 - +0.2 206,509 - -1.0 206,652 + -0.5 206,653 - -0.5 206,653 - +0.5 207,127 + GFF4569 0.37 -1.4 207,127 + GFF4569 0.37 +0.8 207,127 + GFF4569 0.37 +0.3 207,127 + GFF4569 0.37 +0.1 207,127 + GFF4569 0.37 +0.0 207,127 + GFF4569 0.37 +1.7 207,127 + GFF4569 0.37 +1.5 207,127 + GFF4569 0.37 +0.6 207,127 + GFF4569 0.37 -2.0 207,127 + GFF4569 0.37 +0.9 207,127 + GFF4569 0.37 +1.6 207,128 - GFF4569 0.37 +0.8 207,128 - GFF4569 0.37 -0.5 207,128 - GFF4569 0.37 -0.5 207,128 - GFF4569 0.37 -0.1 207,128 - GFF4569 0.37 -0.6 207,169 + GFF4569 0.41 -0.1 207,170 - GFF4569 0.41 -0.8 207,170 - GFF4569 0.41 +0.7 207,250 + GFF4569 0.47 -1.5 207,251 - GFF4569 0.47 +0.1 207,370 + GFF4569 0.57 +0.2 207,371 - GFF4569 0.58 +0.2 207,371 - GFF4569 0.58 -0.4 207,371 - GFF4569 0.58 -0.3 207,371 - GFF4569 0.58 +3.0 207,371 - GFF4569 0.58 +0.2 207,371 - GFF4569 0.58 -0.6 207,670 + GFF4569 0.83 -1.0 207,670 + GFF4569 0.83 +1.2 207,670 + GFF4569 0.83 -0.5 207,671 - GFF4569 0.83 -0.2 207,811 + +0.5 207,811 + -0.2 207,812 - -0.3 207,812 - +1.4 207,871 + +0.3 207,872 - -1.7 208,196 + GFF4570 0.26 -2.4 208,196 + GFF4570 0.26 +0.1 208,197 - GFF4570 0.26 +0.0 208,269 - GFF4570 0.32 +1.0 208,349 + GFF4570 0.38 +0.4 208,349 + GFF4570 0.38 -1.9 208,349 + GFF4570 0.38 +0.4 208,350 - GFF4570 0.38 -1.6 208,350 - GFF4570 0.38 -1.0 208,350 - GFF4570 0.38 -0.2 208,350 - GFF4570 0.38 +0.2 208,350 - GFF4570 0.38 -0.3 208,350 - GFF4570 0.38 +0.7 208,350 - GFF4570 0.38 -0.5 208,350 - GFF4570 0.38 +0.1 208,507 + GFF4570 0.51 -0.4 208,507 + GFF4570 0.51 -1.4 208,508 - GFF4570 0.51 -1.8 208,613 + GFF4570 0.60 +0.2 208,614 - GFF4570 0.60 -0.6 208,614 - GFF4570 0.60 -0.5 208,614 - GFF4570 0.60 +0.1 208,625 + GFF4570 0.61 +2.8 208,626 - GFF4570 0.61 -0.2 208,626 - GFF4570 0.61 +0.8 208,626 - GFF4570 0.61 -0.7
Or see this region's nucleotide sequence