Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4461 and GFF4462 are separated by 66 nucleotides GFF4462 and GFF4463 are separated by 1 nucleotides GFF4463 and GFF4464 are separated by 0 nucleotides GFF4464 and GFF4465 are separated by 48 nucleotides
GFF4461 - hypothetical protein, at 101,487 to 103,649
GFF4461
GFF4462 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 103,716 to 104,264
GFF4462
GFF4463 - alpha-methyl-L-serine aldolase, at 104,266 to 105,534
GFF4463
GFF4464 - hypothetical protein, at 105,535 to 105,660
GFF4464
GFF4465 - Transcriptional regulator, LysR family, at 105,709 to 106,665
GFF4465
Position (kb) 104
105
106 Strain fitness (log2 ratio) -2
-1
0
1 at 103.267 kb on + strand, within GFF4461 at 103.563 kb on + strand at 103.563 kb on + strand at 103.564 kb on - strand at 103.619 kb on - strand at 103.702 kb on + strand at 103.711 kb on + strand at 103.711 kb on + strand at 103.711 kb on + strand at 103.711 kb on + strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.795 kb on - strand, within GFF4462 at 104.025 kb on + strand, within GFF4462 at 104.241 kb on + strand at 104.241 kb on + strand at 104.279 kb on + strand at 104.279 kb on + strand at 104.279 kb on + strand at 104.280 kb on - strand at 104.280 kb on - strand at 104.280 kb on - strand at 104.280 kb on - strand at 104.660 kb on + strand, within GFF4463 at 104.660 kb on + strand, within GFF4463 at 104.660 kb on + strand, within GFF4463 at 104.661 kb on - strand, within GFF4463 at 104.661 kb on - strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.829 kb on - strand, within GFF4463 at 104.829 kb on - strand, within GFF4463 at 104.829 kb on - strand, within GFF4463 at 105.062 kb on + strand, within GFF4463 at 105.206 kb on + strand, within GFF4463 at 105.206 kb on + strand, within GFF4463 at 105.207 kb on - strand, within GFF4463 at 105.207 kb on - strand, within GFF4463 at 105.326 kb on + strand, within GFF4463 at 105.326 kb on + strand, within GFF4463 at 105.327 kb on - strand, within GFF4463 at 105.327 kb on - strand, within GFF4463 at 105.327 kb on - strand, within GFF4463 at 105.903 kb on - strand, within GFF4465 at 105.903 kb on - strand, within GFF4465 at 105.903 kb on - strand, within GFF4465 at 105.903 kb on - strand, within GFF4465 at 106.175 kb on - strand, within GFF4465 at 106.453 kb on + strand, within GFF4465 at 106.453 kb on + strand, within GFF4465
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 103,267 + GFF4461 0.82 +0.2 103,563 + -0.4 103,563 + -0.0 103,564 - +0.2 103,619 - -1.7 103,702 + +0.0 103,711 + +0.1 103,711 + +0.4 103,711 + -0.3 103,711 + +0.1 103,712 - +0.2 103,712 - -0.2 103,712 - -0.2 103,712 - -0.4 103,712 - +0.2 103,712 - +1.6 103,795 - GFF4462 0.14 -1.2 104,025 + GFF4462 0.56 -1.0 104,241 + +0.2 104,241 + -0.3 104,279 + -0.2 104,279 + +0.9 104,279 + -0.5 104,280 - -1.7 104,280 - +0.2 104,280 - -0.2 104,280 - +0.4 104,660 + GFF4463 0.31 -0.7 104,660 + GFF4463 0.31 -0.7 104,660 + GFF4463 0.31 -0.5 104,661 - GFF4463 0.31 -0.7 104,661 - GFF4463 0.31 -1.2 104,828 + GFF4463 0.44 -0.2 104,828 + GFF4463 0.44 +0.4 104,828 + GFF4463 0.44 -1.3 104,828 + GFF4463 0.44 -0.9 104,829 - GFF4463 0.44 -0.9 104,829 - GFF4463 0.44 -0.1 104,829 - GFF4463 0.44 -0.6 105,062 + GFF4463 0.63 +0.3 105,206 + GFF4463 0.74 -0.6 105,206 + GFF4463 0.74 -1.8 105,207 - GFF4463 0.74 +0.1 105,207 - GFF4463 0.74 -0.1 105,326 + GFF4463 0.84 -0.5 105,326 + GFF4463 0.84 -1.6 105,327 - GFF4463 0.84 -0.2 105,327 - GFF4463 0.84 +1.4 105,327 - GFF4463 0.84 +0.2 105,903 - GFF4465 0.20 -0.2 105,903 - GFF4465 0.20 -0.9 105,903 - GFF4465 0.20 -0.7 105,903 - GFF4465 0.20 +1.5 106,175 - GFF4465 0.49 +0.4 106,453 + GFF4465 0.78 +0.1 106,453 + GFF4465 0.78 -1.1
Or see this region's nucleotide sequence