Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4356 and GFF4357 overlap by 4 nucleotides GFF4357 and GFF4358 are separated by 49 nucleotides GFF4358 and GFF4359 are separated by 180 nucleotides GFF4359 and GFF4360 are separated by 7 nucleotides GFF4360 and GFF4361 are separated by 99 nucleotides
GFF4356 - Alpha-methylacyl-CoA racemase (EC 5.1.99.4), at 279,286 to 280,458
GFF4356
GFF4357 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 280,455 to 281,240
GFF4357
GFF4358 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 281,290 to 282,078
GFF4358
GFF4359 - no description, at 282,259 to 282,576
GFF4359
GFF4360 - no description, at 282,584 to 282,853
GFF4360
GFF4361 - Gamma-glutamyltranspeptidase (EC 2.3.2.2) @ Glutathione hydrolase (EC 3.4.19.13), at 282,953 to 284,584
GFF4361
Position (kb) 281
282
283 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 280.370 kb on + strand at 280.660 kb on - strand, within GFF4357 at 281.755 kb on + strand, within GFF4358 at 281.756 kb on - strand, within GFF4358 at 281.756 kb on - strand, within GFF4358 at 281.756 kb on - strand, within GFF4358 at 281.756 kb on - strand, within GFF4358 at 282.123 kb on + strand at 282.135 kb on + strand at 282.557 kb on + strand at 282.558 kb on - strand at 282.558 kb on - strand at 282.558 kb on - strand at 282.660 kb on + strand, within GFF4360 at 282.661 kb on - strand, within GFF4360 at 283.034 kb on + strand at 283.034 kb on + strand at 283.066 kb on - strand at 283.066 kb on - strand at 283.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 280,370 + +1.5 280,660 - GFF4357 0.26 +0.3 281,755 + GFF4358 0.59 -0.2 281,756 - GFF4358 0.59 -0.5 281,756 - GFF4358 0.59 +1.4 281,756 - GFF4358 0.59 +2.5 281,756 - GFF4358 0.59 +0.5 282,123 + -0.5 282,135 + -0.0 282,557 + -0.2 282,558 - -2.8 282,558 - +0.2 282,558 - +0.5 282,660 + GFF4360 0.28 +0.8 282,661 - GFF4360 0.29 -1.3 283,034 + +0.5 283,034 + -1.8 283,066 - -0.2 283,066 - +2.5 283,071 + +0.2
Or see this region's nucleotide sequence