Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4313 and GFF4314 are separated by 18 nucleotides GFF4314 and GFF4315 overlap by 4 nucleotides GFF4315 and GFF4316 overlap by 4 nucleotides
GFF4313 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 233,836 to 234,840
GFF4313
GFF4314 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 234,859 to 235,662
GFF4314
GFF4315 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 235,659 to 236,528
GFF4315
GFF4316 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 236,525 to 238,327
GFF4316
Position (kb) 235
236
237 Strain fitness (log2 ratio) -2
-1
0
1
2 at 234.824 kb on + strand at 234.824 kb on + strand at 234.825 kb on - strand at 234.915 kb on - strand at 235.128 kb on - strand, within GFF4314 at 235.157 kb on + strand, within GFF4314 at 235.157 kb on + strand, within GFF4314 at 235.181 kb on + strand, within GFF4314 at 235.181 kb on + strand, within GFF4314 at 235.182 kb on - strand, within GFF4314 at 235.182 kb on - strand, within GFF4314 at 235.601 kb on + strand at 235.754 kb on - strand, within GFF4315 at 235.831 kb on + strand, within GFF4315 at 236.417 kb on - strand, within GFF4315 at 236.489 kb on + strand at 236.490 kb on - strand at 236.490 kb on - strand at 236.559 kb on + strand at 236.560 kb on - strand at 236.560 kb on - strand at 236.560 kb on - strand at 236.681 kb on + strand at 236.682 kb on - strand at 237.284 kb on + strand, within GFF4316
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 234,824 + +0.6 234,824 + -0.8 234,825 - +1.8 234,915 - +0.2 235,128 - GFF4314 0.33 -0.0 235,157 + GFF4314 0.37 +0.5 235,157 + GFF4314 0.37 -0.7 235,181 + GFF4314 0.40 -1.3 235,181 + GFF4314 0.40 +0.3 235,182 - GFF4314 0.40 +0.2 235,182 - GFF4314 0.40 -0.2 235,601 + +0.8 235,754 - GFF4315 0.11 +0.4 235,831 + GFF4315 0.20 +0.6 236,417 - GFF4315 0.87 +0.5 236,489 + -1.2 236,490 - -0.6 236,490 - -1.9 236,559 + +0.8 236,560 - -0.6 236,560 - -1.3 236,560 - -1.6 236,681 + -0.1 236,682 - -0.5 237,284 + GFF4316 0.42 -0.2
Or see this region's nucleotide sequence