Strain Fitness in Variovorax sp. SCN45 around GFF4298

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4296 and GFF4297 are separated by 22 nucleotidesGFF4297 and GFF4298 are separated by 42 nucleotidesGFF4298 and GFF4299 are separated by 58 nucleotides GFF4296 - hypothetical protein, at 214,772 to 216,310 GFF4296 GFF4297 - 2-ketocyclohexanecarboxyl-CoA hydrolase, at 216,333 to 217,127 GFF4297 GFF4298 - 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase, at 217,170 to 217,946 GFF4298 GFF4299 - 3-ketoacyl-CoA thiolase, at 218,005 to 219,210 GFF4299 Position (kb) 217 218Strain fitness (log2 ratio) -1 0 1at 216.409 kb on + strandat 216.409 kb on + strandat 216.409 kb on + strandat 216.412 kb on + strandat 216.412 kb on + strandat 216.413 kb on - strand, within GFF4297at 216.571 kb on - strand, within GFF4297at 216.613 kb on + strand, within GFF4297at 216.613 kb on + strand, within GFF4297at 216.614 kb on - strand, within GFF4297at 216.890 kb on - strand, within GFF4297at 217.049 kb on - strandat 217.115 kb on - strandat 217.474 kb on + strand, within GFF4298at 218.135 kb on + strand, within GFF4299at 218.136 kb on - strand, within GFF4299at 218.144 kb on + strand, within GFF4299at 218.580 kb on - strand, within GFF4299at 218.747 kb on + strand, within GFF4299

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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216,409 + -0.3
216,409 + -0.3
216,409 + +0.5
216,412 + -0.4
216,412 + -0.7
216,413 - GFF4297 0.10 +0.4
216,571 - GFF4297 0.30 +0.2
216,613 + GFF4297 0.35 -0.7
216,613 + GFF4297 0.35 -0.4
216,614 - GFF4297 0.35 +0.4
216,890 - GFF4297 0.70 -0.6
217,049 - +0.6
217,115 - +0.2
217,474 + GFF4298 0.39 -1.6
218,135 + GFF4299 0.11 -0.9
218,136 - GFF4299 0.11 -1.4
218,144 + GFF4299 0.12 +1.3
218,580 - GFF4299 0.48 +0.6
218,747 + GFF4299 0.62 -0.9

Or see this region's nucleotide sequence