Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4042 and GFF4043 overlap by 4 nucleotides GFF4043 and GFF4044 are separated by 807 nucleotides
GFF4042 - Copper-sensing two-component system response regulator CusR, at 253,094 to 253,777
GFF4042
GFF4043 - Copper sensory histidine kinase CusS, at 253,774 to 255,102
GFF4043
GFF4044 - hypothetical protein, at 255,910 to 256,335
GFF4044
Position (kb)
253
254
255
256 Strain fitness (log2 ratio)
-1
0
1
2 at 252.981 kb on - strand at 252.981 kb on - strand at 252.990 kb on + strand at 252.990 kb on + strand at 252.990 kb on + strand at 252.990 kb on + strand at 252.991 kb on - strand at 253.057 kb on + strand at 253.057 kb on + strand at 253.137 kb on - strand at 253.137 kb on - strand at 253.226 kb on + strand, within GFF4042 at 253.227 kb on - strand, within GFF4042 at 253.383 kb on - strand, within GFF4042 at 253.383 kb on - strand, within GFF4042 at 253.747 kb on - strand at 253.747 kb on - strand at 253.915 kb on + strand, within GFF4043 at 253.918 kb on - strand, within GFF4043 at 254.054 kb on - strand, within GFF4043 at 254.054 kb on - strand, within GFF4043 at 255.012 kb on + strand at 255.013 kb on - strand at 255.013 kb on - strand at 255.013 kb on - strand at 255.117 kb on - strand at 255.117 kb on - strand at 255.117 kb on - strand at 255.258 kb on + strand at 255.510 kb on + strand at 255.510 kb on + strand at 255.511 kb on - strand at 255.544 kb on - strand at 255.636 kb on - strand at 255.722 kb on + strand at 255.723 kb on - strand at 255.727 kb on - strand at 255.747 kb on - strand at 255.747 kb on - strand at 255.846 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 252,981 - -0.4 252,981 - +0.7 252,990 + +1.2 252,990 + -0.6 252,990 + -1.0 252,990 + -0.6 252,991 - -0.3 253,057 + -0.1 253,057 + +0.5 253,137 - -0.0 253,137 - +0.1 253,226 + GFF4042 0.19 -0.0 253,227 - GFF4042 0.19 -0.4 253,383 - GFF4042 0.42 -0.1 253,383 - GFF4042 0.42 +0.7 253,747 - -0.5 253,747 - +0.6 253,915 + GFF4043 0.11 -0.2 253,918 - GFF4043 0.11 +1.9 254,054 - GFF4043 0.21 +0.3 254,054 - GFF4043 0.21 +0.6 255,012 + +0.3 255,013 - -0.8 255,013 - +0.1 255,013 - -0.7 255,117 - -1.3 255,117 - +0.3 255,117 - +0.8 255,258 + -0.3 255,510 + -0.3 255,510 + +0.7 255,511 - +0.2 255,544 - +0.2 255,636 - -1.7 255,722 + -0.5 255,723 - -1.6 255,727 - -0.5 255,747 - -0.6 255,747 - -1.1 255,846 + -0.2
Or see this region's nucleotide sequence