Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3981 and GFF3982 are separated by 34 nucleotides GFF3982 and GFF3983 overlap by 4 nucleotides
GFF3981 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 184,352 to 185,515
GFF3981
GFF3982 - Branched-chain amino acid ABC transporter, permease protein LivH (TC 3.A.1.4.1), at 185,550 to 186,605
GFF3982
GFF3983 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 186,602 to 188,389
GFF3983
Position (kb)
185
186
187 Strain fitness (log2 ratio)
-1
0
1 at 184.844 kb on + strand, within GFF3981 at 184.844 kb on + strand, within GFF3981 at 184.880 kb on + strand, within GFF3981 at 184.881 kb on - strand, within GFF3981 at 185.070 kb on - strand, within GFF3981 at 185.085 kb on - strand, within GFF3981 at 185.198 kb on + strand, within GFF3981 at 185.198 kb on + strand, within GFF3981 at 185.198 kb on + strand, within GFF3981 at 185.423 kb on + strand at 185.423 kb on + strand at 185.423 kb on + strand at 185.423 kb on + strand at 185.424 kb on - strand at 185.424 kb on - strand at 185.424 kb on - strand at 185.608 kb on - strand at 186.300 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.364 kb on - strand, within GFF3982 at 186.364 kb on - strand, within GFF3982 at 186.367 kb on - strand, within GFF3982 at 187.076 kb on + strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.079 kb on + strand, within GFF3983 at 187.080 kb on - strand, within GFF3983 at 187.271 kb on + strand, within GFF3983 at 187.271 kb on + strand, within GFF3983 at 187.272 kb on - strand, within GFF3983 at 187.331 kb on + strand, within GFF3983
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 184,844 + GFF3981 0.42 -0.2 184,844 + GFF3981 0.42 -0.3 184,880 + GFF3981 0.45 -1.2 184,881 - GFF3981 0.45 -0.6 185,070 - GFF3981 0.62 -1.1 185,085 - GFF3981 0.63 -0.1 185,198 + GFF3981 0.73 +0.2 185,198 + GFF3981 0.73 -0.8 185,198 + GFF3981 0.73 +0.9 185,423 + -0.0 185,423 + +0.8 185,423 + +0.7 185,423 + -1.1 185,424 - +0.2 185,424 - -0.3 185,424 - -1.2 185,608 - -0.4 186,300 + GFF3982 0.71 -0.8 186,363 + GFF3982 0.77 +0.5 186,363 + GFF3982 0.77 -0.7 186,363 + GFF3982 0.77 -0.3 186,363 + GFF3982 0.77 +0.9 186,363 + GFF3982 0.77 -1.0 186,363 + GFF3982 0.77 +0.1 186,364 - GFF3982 0.77 -0.6 186,364 - GFF3982 0.77 -1.5 186,367 - GFF3982 0.77 -0.9 187,076 + GFF3983 0.27 -1.5 187,077 - GFF3983 0.27 +0.3 187,077 - GFF3983 0.27 +1.2 187,077 - GFF3983 0.27 +0.6 187,079 + GFF3983 0.27 +0.1 187,080 - GFF3983 0.27 +0.1 187,271 + GFF3983 0.37 -0.0 187,271 + GFF3983 0.37 +0.2 187,272 - GFF3983 0.37 +1.1 187,331 + GFF3983 0.41 +0.1
Or see this region's nucleotide sequence