Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3974 and GFF3975 are separated by 16 nucleotides GFF3975 and GFF3976 overlap by 4 nucleotides GFF3976 and GFF3977 are separated by 49 nucleotides
GFF3974 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 177,317 to 178,570
GFF3974
GFF3975 - Metal-dependent hydrolase, at 178,587 to 179,399
GFF3975
GFF3976 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 179,396 to 180,130
GFF3976
GFF3977 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 180,180 to 181,346
GFF3977
Position (kb) 179
180
181 Strain fitness (log2 ratio) -2
-1
0
1
2 at 178.485 kb on - strand at 178.968 kb on + strand, within GFF3975 at 178.968 kb on + strand, within GFF3975 at 179.212 kb on - strand, within GFF3975 at 179.212 kb on - strand, within GFF3975 at 179.230 kb on - strand, within GFF3975 at 179.241 kb on + strand, within GFF3975 at 179.241 kb on + strand, within GFF3975 at 179.242 kb on - strand, within GFF3975 at 179.242 kb on - strand, within GFF3975 at 179.242 kb on - strand, within GFF3975 at 179.244 kb on + strand, within GFF3975 at 179.244 kb on + strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.825 kb on + strand, within GFF3976 at 179.825 kb on + strand, within GFF3976 at 179.825 kb on + strand, within GFF3976 at 179.863 kb on + strand, within GFF3976 at 179.943 kb on - strand, within GFF3976 at 180.089 kb on + strand at 180.090 kb on - strand at 180.090 kb on - strand at 180.090 kb on - strand at 180.090 kb on - strand at 180.325 kb on - strand, within GFF3977 at 180.354 kb on + strand, within GFF3977 at 180.355 kb on - strand, within GFF3977 at 180.708 kb on + strand, within GFF3977 at 180.898 kb on - strand, within GFF3977 at 180.913 kb on - strand, within GFF3977 at 180.913 kb on - strand, within GFF3977 at 181.111 kb on - strand, within GFF3977 at 181.111 kb on - strand, within GFF3977
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 178,485 - -0.3 178,968 + GFF3975 0.47 +0.0 178,968 + GFF3975 0.47 -1.5 179,212 - GFF3975 0.77 -1.0 179,212 - GFF3975 0.77 -0.3 179,230 - GFF3975 0.79 -0.3 179,241 + GFF3975 0.80 +2.0 179,241 + GFF3975 0.80 -0.1 179,242 - GFF3975 0.81 +0.3 179,242 - GFF3975 0.81 -0.3 179,242 - GFF3975 0.81 +1.1 179,244 + GFF3975 0.81 +0.7 179,244 + GFF3975 0.81 -0.8 179,245 - GFF3975 0.81 +0.9 179,245 - GFF3975 0.81 -0.1 179,245 - GFF3975 0.81 -1.7 179,245 - GFF3975 0.81 -1.1 179,825 + GFF3976 0.58 +1.1 179,825 + GFF3976 0.58 -1.1 179,825 + GFF3976 0.58 -2.1 179,863 + GFF3976 0.64 -0.4 179,943 - GFF3976 0.74 -1.8 180,089 + -0.8 180,090 - -2.5 180,090 - +1.4 180,090 - +0.0 180,090 - +0.2 180,325 - GFF3977 0.12 -1.7 180,354 + GFF3977 0.15 +0.0 180,355 - GFF3977 0.15 -0.1 180,708 + GFF3977 0.45 +0.2 180,898 - GFF3977 0.62 -1.1 180,913 - GFF3977 0.63 -1.3 180,913 - GFF3977 0.63 +0.9 181,111 - GFF3977 0.80 -1.5 181,111 - GFF3977 0.80 +0.6
Or see this region's nucleotide sequence