Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3699 and GFF3700 are separated by 59 nucleotides GFF3700 and GFF3701 are separated by 126 nucleotides GFF3701 and GFF3702 are separated by 5 nucleotides GFF3702 and GFF3703 are separated by 126 nucleotides
GFF3699 - Thioredoxin 2, at 200,809 to 201,258
GFF3699
GFF3700 - FIG006285: hypothetical protein, at 201,318 to 202,016
GFF3700
GFF3701 - FIG004694: Hypothetical protein, at 202,143 to 202,670
GFF3701
GFF3702 - Beta-propeller domains of methanol dehydrogenase type, at 202,676 to 203,533
GFF3702
GFF3703 - LemA family protein, at 203,660 to 204,259
GFF3703
Position (kb)
202
203 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 201.483 kb on + strand, within GFF3700 at 202.152 kb on + strand at 202.152 kb on + strand at 202.152 kb on + strand at 202.153 kb on - strand at 202.153 kb on - strand at 202.153 kb on - strand at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.480 kb on + strand, within GFF3701 at 202.480 kb on + strand, within GFF3701 at 202.481 kb on - strand, within GFF3701 at 202.481 kb on - strand, within GFF3701 at 202.481 kb on - strand, within GFF3701 at 203.328 kb on + strand, within GFF3702 at 203.661 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 201,483 + GFF3700 0.24 +0.1 202,152 + -0.0 202,152 + -1.6 202,152 + +0.1 202,153 - -0.4 202,153 - -1.7 202,153 - -1.5 202,384 + GFF3701 0.46 +1.9 202,384 + GFF3701 0.46 +0.4 202,384 + GFF3701 0.46 +0.2 202,384 + GFF3701 0.46 +0.1 202,384 + GFF3701 0.46 +0.1 202,385 - GFF3701 0.46 +0.6 202,385 - GFF3701 0.46 -0.6 202,385 - GFF3701 0.46 +0.4 202,385 - GFF3701 0.46 -0.7 202,480 + GFF3701 0.64 +0.1 202,480 + GFF3701 0.64 -0.2 202,481 - GFF3701 0.64 -0.4 202,481 - GFF3701 0.64 -2.9 202,481 - GFF3701 0.64 +0.3 203,328 + GFF3702 0.76 -0.3 203,661 + +0.3
Or see this region's nucleotide sequence