Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3454 and GFF3455 are separated by 15 nucleotides GFF3455 and GFF3456 are separated by 2 nucleotides
GFF3454 - Cys regulon transcriptional activator CysB, at 275,982 to 276,872
GFF3454
GFF3455 - Two component response regulator associated with urea and amide use, at 276,888 to 277,538
GFF3455
GFF3456 - Possible regulatory protein similar to urea ABC transporter, substrate binding protein, at 277,541 to 278,695
GFF3456
Position (kb)
276
277
278 Strain fitness (log2 ratio)
-2
-1
0
1 at 275.904 kb on - strand at 275.949 kb on + strand at 276.378 kb on + strand, within GFF3454 at 276.378 kb on + strand, within GFF3454 at 276.379 kb on - strand, within GFF3454 at 276.588 kb on + strand, within GFF3454 at 276.588 kb on + strand, within GFF3454 at 276.588 kb on + strand, within GFF3454 at 276.588 kb on + strand, within GFF3454 at 276.588 kb on + strand, within GFF3454 at 276.589 kb on - strand, within GFF3454 at 276.589 kb on - strand, within GFF3454 at 276.713 kb on - strand, within GFF3454 at 276.957 kb on + strand, within GFF3455 at 276.957 kb on + strand, within GFF3455 at 276.958 kb on - strand, within GFF3455 at 276.958 kb on - strand, within GFF3455 at 276.958 kb on - strand, within GFF3455 at 277.476 kb on + strand at 277.476 kb on + strand at 277.476 kb on + strand at 277.600 kb on - strand at 277.600 kb on - strand at 277.827 kb on + strand, within GFF3456 at 277.828 kb on - strand, within GFF3456 at 278.046 kb on + strand, within GFF3456 at 278.047 kb on - strand, within GFF3456 at 278.169 kb on + strand, within GFF3456 at 278.250 kb on + strand, within GFF3456 at 278.251 kb on - strand, within GFF3456 at 278.251 kb on - strand, within GFF3456 at 278.269 kb on - strand, within GFF3456 at 278.344 kb on - strand, within GFF3456 at 278.362 kb on - strand, within GFF3456 at 278.376 kb on + strand, within GFF3456 at 278.377 kb on - strand, within GFF3456 at 278.377 kb on - strand, within GFF3456 at 278.451 kb on + strand, within GFF3456 at 278.452 kb on - strand, within GFF3456 at 278.452 kb on - strand, within GFF3456
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 275,904 - +0.8 275,949 + -0.6 276,378 + GFF3454 0.44 -0.2 276,378 + GFF3454 0.44 +0.3 276,379 - GFF3454 0.45 -1.7 276,588 + GFF3454 0.68 +0.8 276,588 + GFF3454 0.68 +0.1 276,588 + GFF3454 0.68 -1.5 276,588 + GFF3454 0.68 -1.2 276,588 + GFF3454 0.68 +0.2 276,589 - GFF3454 0.68 -2.2 276,589 - GFF3454 0.68 +1.2 276,713 - GFF3454 0.82 +0.6 276,957 + GFF3455 0.11 -0.9 276,957 + GFF3455 0.11 -1.3 276,958 - GFF3455 0.11 -0.5 276,958 - GFF3455 0.11 +1.4 276,958 - GFF3455 0.11 -2.7 277,476 + -0.5 277,476 + +0.9 277,476 + +0.9 277,600 - -1.2 277,600 - +0.6 277,827 + GFF3456 0.25 +0.1 277,828 - GFF3456 0.25 +0.4 278,046 + GFF3456 0.44 +0.5 278,047 - GFF3456 0.44 -0.1 278,169 + GFF3456 0.54 -1.0 278,250 + GFF3456 0.61 +0.7 278,251 - GFF3456 0.61 +0.1 278,251 - GFF3456 0.61 -2.6 278,269 - GFF3456 0.63 -0.3 278,344 - GFF3456 0.70 -0.4 278,362 - GFF3456 0.71 -0.7 278,376 + GFF3456 0.72 -0.7 278,377 - GFF3456 0.72 +0.6 278,377 - GFF3456 0.72 -0.2 278,451 + GFF3456 0.79 -0.6 278,452 - GFF3456 0.79 -0.9 278,452 - GFF3456 0.79 +0.0
Or see this region's nucleotide sequence