Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3444 and GFF3445 are separated by 64 nucleotides GFF3445 and GFF3446 are separated by 24 nucleotides GFF3446 and GFF3447 are separated by 48 nucleotides GFF3447 and GFF3448 overlap by 4 nucleotides
GFF3444 - BUG/TctC family periplasmic protein, at 267,348 to 268,337
GFF3444
GFF3445 - Cupin 2, conserved barrel, at 268,402 to 268,761
GFF3445
GFF3446 - Quinone oxidoreductase (EC 1.6.5.5), at 268,786 to 269,793
GFF3446
GFF3447 - FIG00555764: hypothetical protein, at 269,842 to 270,732
GFF3447
GFF3448 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 270,729 to 271,493
GFF3448
Position (kb) 268
269
270 Strain fitness (log2 ratio) -1
0
1 at 267.856 kb on + strand, within GFF3444 at 267.856 kb on + strand, within GFF3444 at 267.856 kb on + strand, within GFF3444 at 267.857 kb on - strand, within GFF3444 at 267.857 kb on - strand, within GFF3444 at 267.992 kb on - strand, within GFF3444 at 268.007 kb on - strand, within GFF3444 at 268.054 kb on + strand, within GFF3444 at 268.508 kb on + strand, within GFF3445 at 268.584 kb on - strand, within GFF3445 at 269.091 kb on - strand, within GFF3446 at 269.091 kb on - strand, within GFF3446 at 269.111 kb on + strand, within GFF3446 at 269.139 kb on + strand, within GFF3446 at 269.510 kb on + strand, within GFF3446 at 269.511 kb on - strand, within GFF3446 at 269.981 kb on + strand, within GFF3447 at 269.981 kb on + strand, within GFF3447 at 270.111 kb on - strand, within GFF3447 at 270.143 kb on + strand, within GFF3447 at 270.143 kb on + strand, within GFF3447 at 270.144 kb on - strand, within GFF3447 at 270.144 kb on - strand, within GFF3447 at 270.144 kb on - strand, within GFF3447 at 270.537 kb on - strand, within GFF3447
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 267,856 + GFF3444 0.51 -1.7 267,856 + GFF3444 0.51 +1.1 267,856 + GFF3444 0.51 -0.4 267,857 - GFF3444 0.51 -0.6 267,857 - GFF3444 0.51 +1.1 267,992 - GFF3444 0.65 +0.3 268,007 - GFF3444 0.67 -0.4 268,054 + GFF3444 0.71 +1.1 268,508 + GFF3445 0.29 -0.4 268,584 - GFF3445 0.51 -0.8 269,091 - GFF3446 0.30 +0.8 269,091 - GFF3446 0.30 +0.7 269,111 + GFF3446 0.32 -0.2 269,139 + GFF3446 0.35 +0.3 269,510 + GFF3446 0.72 -0.6 269,511 - GFF3446 0.72 -1.6 269,981 + GFF3447 0.16 +0.6 269,981 + GFF3447 0.16 +0.0 270,111 - GFF3447 0.30 +0.7 270,143 + GFF3447 0.34 -0.5 270,143 + GFF3447 0.34 -0.4 270,144 - GFF3447 0.34 -0.3 270,144 - GFF3447 0.34 -0.3 270,144 - GFF3447 0.34 +0.1 270,537 - GFF3447 0.78 +0.3
Or see this region's nucleotide sequence