Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3433 and GFF3434 are separated by 188 nucleotides GFF3434 and GFF3435 are separated by 1 nucleotides GFF3435 and GFF3436 are separated by 67 nucleotides
GFF3433 - hypothetical protein, at 256,903 to 257,334
GFF3433
GFF3434 - Organic hydroperoxide resistance protein, at 257,523 to 257,936
GFF3434
GFF3435 - predicted integral membrane protein, at 257,938 to 258,396
GFF3435
GFF3436 - Transcriptional regulator, LysR family, at 258,464 to 259,432
GFF3436
Position (kb) 257
258
259 Strain fitness (log2 ratio) -2
-1
0
1
2 at 256.993 kb on + strand, within GFF3433 at 257.279 kb on - strand, within GFF3433 at 257.279 kb on - strand, within GFF3433 at 257.279 kb on - strand, within GFF3433 at 257.279 kb on - strand, within GFF3433 at 257.333 kb on - strand at 257.370 kb on + strand at 257.370 kb on + strand at 257.371 kb on - strand at 257.371 kb on - strand at 257.371 kb on - strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.400 kb on - strand at 257.400 kb on - strand at 257.442 kb on + strand at 257.442 kb on + strand at 257.457 kb on + strand at 257.457 kb on + strand at 257.458 kb on - strand at 257.458 kb on - strand at 257.544 kb on + strand at 257.544 kb on + strand at 257.545 kb on - strand at 257.793 kb on + strand, within GFF3434 at 257.793 kb on + strand, within GFF3434 at 257.793 kb on + strand, within GFF3434 at 257.794 kb on - strand, within GFF3434 at 257.794 kb on - strand, within GFF3434 at 257.794 kb on - strand, within GFF3434 at 257.886 kb on + strand, within GFF3434 at 257.887 kb on - strand, within GFF3434 at 257.887 kb on - strand, within GFF3434 at 257.887 kb on - strand, within GFF3434 at 258.098 kb on + strand, within GFF3435 at 258.098 kb on + strand, within GFF3435 at 258.098 kb on + strand, within GFF3435 at 258.098 kb on + strand, within GFF3435 at 258.098 kb on + strand, within GFF3435 at 258.099 kb on - strand, within GFF3435 at 258.099 kb on - strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.390 kb on - strand at 258.390 kb on - strand at 258.390 kb on - strand at 258.390 kb on - strand at 258.390 kb on - strand at 258.390 kb on - strand at 258.529 kb on - strand at 258.708 kb on + strand, within GFF3436 at 258.708 kb on + strand, within GFF3436 at 259.119 kb on + strand, within GFF3436 at 259.119 kb on + strand, within GFF3436 at 259.120 kb on - strand, within GFF3436 at 259.221 kb on + strand, within GFF3436 at 259.221 kb on + strand, within GFF3436 at 259.222 kb on - strand, within GFF3436
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 256,993 + GFF3433 0.21 +0.2 257,279 - GFF3433 0.87 +0.2 257,279 - GFF3433 0.87 +0.5 257,279 - GFF3433 0.87 -0.2 257,279 - GFF3433 0.87 +0.3 257,333 - -0.2 257,370 + -0.1 257,370 + -0.4 257,371 - -0.2 257,371 - -0.1 257,371 - -0.7 257,399 + -1.1 257,399 + -0.0 257,399 + +0.9 257,399 + -0.6 257,399 + -0.1 257,399 + -2.1 257,399 + +0.1 257,399 + -0.8 257,400 - -0.9 257,400 - +0.0 257,442 + -0.7 257,442 + +0.4 257,457 + -0.3 257,457 + +0.3 257,458 - -0.7 257,458 - +0.8 257,544 + +1.0 257,544 + +0.9 257,545 - -0.7 257,793 + GFF3434 0.65 -0.7 257,793 + GFF3434 0.65 +1.0 257,793 + GFF3434 0.65 -1.8 257,794 - GFF3434 0.65 +0.5 257,794 - GFF3434 0.65 -1.5 257,794 - GFF3434 0.65 +0.7 257,886 + GFF3434 0.88 -0.3 257,887 - GFF3434 0.88 -1.0 257,887 - GFF3434 0.88 +0.0 257,887 - GFF3434 0.88 +1.9 258,098 + GFF3435 0.35 +0.3 258,098 + GFF3435 0.35 +0.2 258,098 + GFF3435 0.35 +1.3 258,098 + GFF3435 0.35 +0.2 258,098 + GFF3435 0.35 +1.9 258,099 - GFF3435 0.35 +0.2 258,099 - GFF3435 0.35 +0.8 258,104 + GFF3435 0.36 +0.3 258,104 + GFF3435 0.36 +0.3 258,104 + GFF3435 0.36 +0.0 258,104 + GFF3435 0.36 -1.1 258,104 + GFF3435 0.36 +0.5 258,104 + GFF3435 0.36 +0.3 258,104 + GFF3435 0.36 -0.9 258,390 - +0.7 258,390 - +2.2 258,390 - +1.0 258,390 - -0.4 258,390 - -0.6 258,390 - +0.7 258,529 - +0.2 258,708 + GFF3436 0.25 +0.0 258,708 + GFF3436 0.25 +0.0 259,119 + GFF3436 0.68 -0.3 259,119 + GFF3436 0.68 -0.4 259,120 - GFF3436 0.68 -1.3 259,221 + GFF3436 0.78 -0.8 259,221 + GFF3436 0.78 -0.2 259,222 - GFF3436 0.78 -0.4
Or see this region's nucleotide sequence