Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3319 and GFF3320 are separated by 35 nucleotides GFF3320 and GFF3321 overlap by 4 nucleotides GFF3321 and GFF3322 are separated by 34 nucleotides
GFF3319 - Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase), at 146,106 to 147,236
GFF3319
GFF3320 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 147,272 to 148,555
GFF3320
GFF3321 - Acyl carrier protein, at 148,552 to 148,857
GFF3321
GFF3322 - hypothetical protein, at 148,892 to 149,752
GFF3322
Position (kb) 147
148
149 Strain fitness (log2 ratio) -2
-1
0
1
2 at 146.503 kb on + strand, within GFF3319 at 146.504 kb on - strand, within GFF3319 at 146.560 kb on + strand, within GFF3319 at 146.591 kb on + strand, within GFF3319 at 146.592 kb on - strand, within GFF3319 at 146.610 kb on + strand, within GFF3319 at 146.610 kb on + strand, within GFF3319 at 146.610 kb on + strand, within GFF3319 at 146.611 kb on - strand, within GFF3319 at 146.611 kb on - strand, within GFF3319 at 146.611 kb on - strand, within GFF3319 at 146.611 kb on - strand, within GFF3319 at 146.629 kb on + strand, within GFF3319 at 146.645 kb on - strand, within GFF3319 at 146.645 kb on - strand, within GFF3319 at 146.645 kb on - strand, within GFF3319 at 146.749 kb on + strand, within GFF3319 at 146.750 kb on - strand, within GFF3319 at 146.818 kb on + strand, within GFF3319 at 146.818 kb on + strand, within GFF3319 at 146.818 kb on + strand, within GFF3319 at 146.818 kb on + strand, within GFF3319 at 146.818 kb on + strand, within GFF3319 at 146.819 kb on - strand, within GFF3319 at 146.819 kb on - strand, within GFF3319 at 146.819 kb on - strand, within GFF3319 at 146.953 kb on + strand, within GFF3319 at 146.953 kb on + strand, within GFF3319 at 147.043 kb on + strand, within GFF3319 at 147.044 kb on - strand, within GFF3319 at 147.055 kb on + strand, within GFF3319 at 147.145 kb on + strand at 147.146 kb on - strand at 147.146 kb on - strand at 148.143 kb on + strand, within GFF3320 at 148.144 kb on - strand, within GFF3320 at 148.560 kb on + strand at 148.561 kb on - strand at 148.899 kb on + strand at 148.899 kb on + strand at 148.900 kb on - strand at 148.900 kb on - strand at 149.001 kb on + strand, within GFF3322 at 149.001 kb on + strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.277 kb on + strand, within GFF3322 at 149.295 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 146,503 + GFF3319 0.35 -1.0 146,504 - GFF3319 0.35 -1.6 146,560 + GFF3319 0.40 -0.4 146,591 + GFF3319 0.43 +0.4 146,592 - GFF3319 0.43 -0.7 146,610 + GFF3319 0.45 +0.1 146,610 + GFF3319 0.45 +0.2 146,610 + GFF3319 0.45 -0.3 146,611 - GFF3319 0.45 +0.3 146,611 - GFF3319 0.45 -0.7 146,611 - GFF3319 0.45 +0.4 146,611 - GFF3319 0.45 -2.1 146,629 + GFF3319 0.46 -0.1 146,645 - GFF3319 0.48 +1.0 146,645 - GFF3319 0.48 -0.9 146,645 - GFF3319 0.48 -0.6 146,749 + GFF3319 0.57 -0.7 146,750 - GFF3319 0.57 -0.3 146,818 + GFF3319 0.63 +0.5 146,818 + GFF3319 0.63 -0.7 146,818 + GFF3319 0.63 -0.4 146,818 + GFF3319 0.63 +0.1 146,818 + GFF3319 0.63 -0.7 146,819 - GFF3319 0.63 +0.1 146,819 - GFF3319 0.63 -0.4 146,819 - GFF3319 0.63 +0.1 146,953 + GFF3319 0.75 +0.6 146,953 + GFF3319 0.75 +0.6 147,043 + GFF3319 0.83 -0.8 147,044 - GFF3319 0.83 +0.5 147,055 + GFF3319 0.84 -2.0 147,145 + +0.0 147,146 - -0.0 147,146 - +0.0 148,143 + GFF3320 0.68 -0.9 148,144 - GFF3320 0.68 -0.4 148,560 + -0.6 148,561 - +2.5 148,899 + +0.4 148,899 + -0.2 148,900 - -0.3 148,900 - -0.0 149,001 + GFF3322 0.13 +0.4 149,001 + GFF3322 0.13 -0.1 149,002 - GFF3322 0.13 +1.6 149,002 - GFF3322 0.13 +0.9 149,002 - GFF3322 0.13 -0.6 149,002 - GFF3322 0.13 +0.4 149,002 - GFF3322 0.13 -0.6 149,277 + GFF3322 0.45 +0.6 149,295 + GFF3322 0.47 -0.4 149,322 + GFF3322 0.50 +0.2 149,322 + GFF3322 0.50 -2.1 149,322 + GFF3322 0.50 -0.8 149,322 + GFF3322 0.50 -0.5 149,322 + GFF3322 0.50 -0.2 149,322 + GFF3322 0.50 -0.3 149,323 - GFF3322 0.50 -1.8 149,323 - GFF3322 0.50 -0.2 149,323 - GFF3322 0.50 -0.5 149,323 - GFF3322 0.50 +0.8 149,323 - GFF3322 0.50 -1.1
Or see this region's nucleotide sequence