Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3263 and GFF3264 are separated by 562 nucleotides GFF3264 and GFF3265 overlap by 4 nucleotides
GFF3263 - Potassium voltage-gated channel subfamily KQT; possible potassium channel, VIC family, at 83,146 to 84,066
GFF3263
GFF3264 - Methionine biosynthesis protein MetW, at 84,629 to 85,219
GFF3264
GFF3265 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 85,216 to 86,340
GFF3265
Position (kb) 84
85
86 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.761 kb on + strand, within GFF3263 at 83.762 kb on - strand, within GFF3263 at 83.762 kb on - strand, within GFF3263 at 84.270 kb on + strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.315 kb on + strand at 84.315 kb on + strand at 84.316 kb on - strand at 84.316 kb on - strand at 84.801 kb on + strand, within GFF3264 at 84.801 kb on + strand, within GFF3264 at 84.801 kb on + strand, within GFF3264 at 84.801 kb on + strand, within GFF3264 at 84.801 kb on + strand, within GFF3264 at 84.802 kb on - strand, within GFF3264 at 84.802 kb on - strand, within GFF3264 at 84.802 kb on - strand, within GFF3264 at 84.802 kb on - strand, within GFF3264 at 84.802 kb on - strand, within GFF3264 at 84.811 kb on - strand, within GFF3264 at 85.271 kb on + strand at 85.486 kb on + strand, within GFF3265 at 85.491 kb on - strand, within GFF3265 at 85.610 kb on + strand, within GFF3265 at 85.691 kb on + strand, within GFF3265 at 85.691 kb on + strand, within GFF3265 at 85.754 kb on + strand, within GFF3265 at 86.028 kb on - strand, within GFF3265
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 83,710 + GFF3263 0.61 -0.5 83,710 + GFF3263 0.61 -0.0 83,710 + GFF3263 0.61 -0.2 83,710 + GFF3263 0.61 -2.1 83,710 + GFF3263 0.61 +0.2 83,710 + GFF3263 0.61 -0.2 83,710 + GFF3263 0.61 -0.8 83,710 + GFF3263 0.61 -0.5 83,710 + GFF3263 0.61 +0.3 83,710 + GFF3263 0.61 -0.2 83,710 + GFF3263 0.61 -0.6 83,710 + GFF3263 0.61 -0.6 83,710 + GFF3263 0.61 -0.1 83,710 + GFF3263 0.61 +0.6 83,711 - GFF3263 0.61 -1.4 83,711 - GFF3263 0.61 -0.4 83,711 - GFF3263 0.61 -0.8 83,711 - GFF3263 0.61 +0.6 83,711 - GFF3263 0.61 -0.2 83,711 - GFF3263 0.61 -0.8 83,711 - GFF3263 0.61 +0.6 83,711 - GFF3263 0.61 +0.2 83,711 - GFF3263 0.61 -0.3 83,761 + GFF3263 0.67 -0.4 83,762 - GFF3263 0.67 -1.4 83,762 - GFF3263 0.67 -0.7 84,270 + +0.6 84,271 - -0.1 84,271 - -2.2 84,271 - -0.0 84,271 - -0.3 84,271 - +0.5 84,271 - +0.0 84,271 - +0.1 84,315 + +0.1 84,315 + +0.3 84,316 - +0.9 84,316 - -0.9 84,801 + GFF3264 0.29 -1.3 84,801 + GFF3264 0.29 -0.7 84,801 + GFF3264 0.29 +0.3 84,801 + GFF3264 0.29 -3.8 84,801 + GFF3264 0.29 -0.7 84,802 - GFF3264 0.29 -2.4 84,802 - GFF3264 0.29 -2.1 84,802 - GFF3264 0.29 -1.3 84,802 - GFF3264 0.29 -1.3 84,802 - GFF3264 0.29 -1.1 84,811 - GFF3264 0.31 -1.3 85,271 + -0.1 85,486 + GFF3265 0.24 -1.4 85,491 - GFF3265 0.24 -3.0 85,610 + GFF3265 0.35 -1.3 85,691 + GFF3265 0.42 -2.2 85,691 + GFF3265 0.42 -1.7 85,754 + GFF3265 0.48 -0.9 86,028 - GFF3265 0.72 -1.6
Or see this region's nucleotide sequence