Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3223 and GFF3224 overlap by 4 nucleotides GFF3224 and GFF3225 overlap by 4 nucleotides GFF3225 and GFF3226 are separated by 90 nucleotides GFF3226 and GFF3227 are separated by 17 nucleotides GFF3227 and GFF3228 are separated by 41 nucleotides
GFF3223 - no description, at 44,390 to 45,007
GFF3223
GFF3224 - no description, at 45,004 to 45,630
GFF3224
GFF3225 - hypothetical protein, at 45,627 to 47,885
GFF3225
GFF3226 - no description, at 47,976 to 48,326
GFF3226
GFF3227 - no description, at 48,344 to 48,694
GFF3227
GFF3228 - hypothetical protein, at 48,736 to 49,665
GFF3228
Position (kb) 45
46
47
48 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 45.566 kb on + strand, within GFF3224 at 45.566 kb on + strand, within GFF3224 at 45.567 kb on - strand, within GFF3224 at 45.928 kb on + strand, within GFF3225 at 46.133 kb on - strand, within GFF3225 at 46.133 kb on - strand, within GFF3225 at 47.024 kb on + strand, within GFF3225 at 47.120 kb on + strand, within GFF3225 at 47.120 kb on + strand, within GFF3225 at 47.120 kb on + strand, within GFF3225 at 47.134 kb on + strand, within GFF3225 at 47.135 kb on - strand, within GFF3225 at 47.135 kb on - strand, within GFF3225 at 47.369 kb on - strand, within GFF3225 at 47.635 kb on + strand, within GFF3225 at 47.636 kb on - strand, within GFF3225 at 47.636 kb on - strand, within GFF3225 at 47.665 kb on + strand at 47.710 kb on + strand at 47.710 kb on + strand at 47.710 kb on + strand at 47.826 kb on + strand at 47.826 kb on + strand at 47.891 kb on + strand at 47.892 kb on - strand at 48.192 kb on + strand, within GFF3226 at 48.192 kb on + strand, within GFF3226 at 48.193 kb on - strand, within GFF3226 at 48.381 kb on + strand, within GFF3227 at 48.381 kb on + strand, within GFF3227 at 48.387 kb on + strand, within GFF3227 at 48.388 kb on - strand, within GFF3227
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 45,566 + GFF3224 0.90 +0.4 45,566 + GFF3224 0.90 -0.5 45,567 - GFF3224 0.90 +0.6 45,928 + GFF3225 0.13 -1.7 46,133 - GFF3225 0.22 -0.5 46,133 - GFF3225 0.22 -0.1 47,024 + GFF3225 0.62 -0.3 47,120 + GFF3225 0.66 -1.1 47,120 + GFF3225 0.66 +1.6 47,120 + GFF3225 0.66 -0.4 47,134 + GFF3225 0.67 -0.4 47,135 - GFF3225 0.67 -0.8 47,135 - GFF3225 0.67 -0.3 47,369 - GFF3225 0.77 -0.6 47,635 + GFF3225 0.89 -0.7 47,636 - GFF3225 0.89 -0.3 47,636 - GFF3225 0.89 -0.5 47,665 + +0.3 47,710 + -0.7 47,710 + -0.3 47,710 + +0.9 47,826 + -0.4 47,826 + -0.8 47,891 + -2.3 47,892 - -0.5 48,192 + GFF3226 0.62 -0.8 48,192 + GFF3226 0.62 -0.6 48,193 - GFF3226 0.62 -0.4 48,381 + GFF3227 0.11 -0.4 48,381 + GFF3227 0.11 +0.2 48,387 + GFF3227 0.12 -0.2 48,388 - GFF3227 0.13 +2.9
Or see this region's nucleotide sequence