Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3152 and GFF3153 overlap by 4 nucleotides GFF3153 and GFF3154 are separated by 188 nucleotides
GFF3152 - hypothetical protein, at 587,719 to 589,104
GFF3152
GFF3153 - Two-component transcriptional response regulator, LuxR family, at 589,101 to 589,757
GFF3153
GFF3154 - no description, at 589,946 to 591,103
GFF3154
Position (kb)
589
590 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 588.358 kb on - strand, within GFF3152 at 588.358 kb on - strand, within GFF3152 at 588.705 kb on - strand, within GFF3152 at 588.705 kb on - strand, within GFF3152 at 588.714 kb on - strand, within GFF3152 at 588.795 kb on - strand, within GFF3152 at 588.795 kb on - strand, within GFF3152 at 589.007 kb on + strand at 589.007 kb on + strand at 589.007 kb on + strand at 589.008 kb on - strand at 589.009 kb on - strand at 589.013 kb on + strand at 589.014 kb on - strand at 589.117 kb on + strand at 589.117 kb on + strand at 589.118 kb on - strand at 589.118 kb on - strand at 589.143 kb on - strand at 589.402 kb on + strand, within GFF3153 at 589.403 kb on - strand, within GFF3153 at 589.523 kb on + strand, within GFF3153 at 589.524 kb on - strand, within GFF3153 at 589.782 kb on + strand at 589.782 kb on + strand at 589.783 kb on - strand at 589.783 kb on - strand at 589.783 kb on - strand at 589.845 kb on + strand at 590.432 kb on + strand, within GFF3154 at 590.433 kb on - strand, within GFF3154 at 590.433 kb on - strand, within GFF3154 at 590.648 kb on + strand, within GFF3154 at 590.648 kb on + strand, within GFF3154 at 590.649 kb on - strand, within GFF3154 at 590.649 kb on - strand, within GFF3154 at 590.649 kb on - strand, within GFF3154 at 590.649 kb on - strand, within GFF3154 at 590.714 kb on + strand, within GFF3154
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 588,358 - GFF3152 0.46 +0.5 588,358 - GFF3152 0.46 +0.5 588,705 - GFF3152 0.71 -0.6 588,705 - GFF3152 0.71 -0.2 588,714 - GFF3152 0.72 -3.5 588,795 - GFF3152 0.78 -0.2 588,795 - GFF3152 0.78 +0.6 589,007 + -0.7 589,007 + -1.2 589,007 + -0.3 589,008 - -0.6 589,009 - +0.7 589,013 + -1.3 589,014 - -1.3 589,117 + +0.8 589,117 + +0.3 589,118 - -0.1 589,118 - -0.3 589,143 - -0.6 589,402 + GFF3153 0.46 -1.2 589,403 - GFF3153 0.46 -2.3 589,523 + GFF3153 0.64 -0.4 589,524 - GFF3153 0.64 -1.7 589,782 + +0.6 589,782 + +1.0 589,783 - -1.5 589,783 - -0.3 589,783 - +1.0 589,845 + -1.3 590,432 + GFF3154 0.42 +0.8 590,433 - GFF3154 0.42 +0.5 590,433 - GFF3154 0.42 -0.4 590,648 + GFF3154 0.61 -0.3 590,648 + GFF3154 0.61 -1.8 590,649 - GFF3154 0.61 -0.9 590,649 - GFF3154 0.61 -0.9 590,649 - GFF3154 0.61 +0.4 590,649 - GFF3154 0.61 +0.2 590,714 + GFF3154 0.66 +0.9
Or see this region's nucleotide sequence