Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3131 and GFF3132 are separated by 41 nucleotides GFF3132 and GFF3133 are separated by 222 nucleotides GFF3133 and GFF3134 are separated by 5 nucleotides GFF3134 and GFF3135 are separated by 88 nucleotides GFF3135 and GFF3136 are separated by 6 nucleotides
GFF3131 - VgrG protein, at 559,559 to 562,732
GFF3131
GFF3132 - T6SS component Hcp, at 562,774 to 563,256
GFF3132
GFF3133 - hypothetical protein, at 563,479 to 564,624
GFF3133
GFF3134 - no description, at 564,630 to 564,902
GFF3134
GFF3135 - no description, at 564,991 to 565,530
GFF3135
GFF3136 - no description, at 565,537 to 566,523
GFF3136
Position (kb) 563
564
565 Strain fitness (log2 ratio) -1
0
1 at 562.494 kb on + strand at 562.719 kb on + strand at 562.925 kb on + strand, within GFF3132 at 563.249 kb on - strand at 563.458 kb on + strand at 563.459 kb on - strand at 563.571 kb on - strand at 563.844 kb on - strand, within GFF3133 at 563.973 kb on - strand, within GFF3133 at 564.273 kb on + strand, within GFF3133 at 564.452 kb on + strand, within GFF3133 at 565.054 kb on + strand, within GFF3135 at 565.055 kb on - strand, within GFF3135 at 565.055 kb on - strand, within GFF3135 at 565.078 kb on + strand, within GFF3135 at 565.079 kb on - strand, within GFF3135 at 565.103 kb on - strand, within GFF3135 at 565.286 kb on + strand, within GFF3135 at 565.287 kb on - strand, within GFF3135 at 565.287 kb on - strand, within GFF3135 at 565.318 kb on + strand, within GFF3135 at 565.377 kb on - strand, within GFF3135
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 562,494 + +1.0 562,719 + -0.0 562,925 + GFF3132 0.31 +0.6 563,249 - +0.5 563,458 + +0.1 563,459 - +1.0 563,571 - -0.3 563,844 - GFF3133 0.32 -1.1 563,973 - GFF3133 0.43 -0.8 564,273 + GFF3133 0.69 -0.5 564,452 + GFF3133 0.85 -1.5 565,054 + GFF3135 0.12 +0.2 565,055 - GFF3135 0.12 -1.0 565,055 - GFF3135 0.12 -0.4 565,078 + GFF3135 0.16 -0.4 565,079 - GFF3135 0.16 -0.1 565,103 - GFF3135 0.21 +0.0 565,286 + GFF3135 0.55 +0.1 565,287 - GFF3135 0.55 -0.2 565,287 - GFF3135 0.55 -1.1 565,318 + GFF3135 0.61 +0.0 565,377 - GFF3135 0.71 -0.1
Or see this region's nucleotide sequence