Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3096 and GFF3097 are separated by 82 nucleotides GFF3097 and GFF3098 are separated by 10 nucleotides
GFF3096 - Argininosuccinate synthase (EC 6.3.4.5), at 525,271 to 526,611
GFF3096
GFF3097 - hypothetical protein, at 526,694 to 527,539
GFF3097
GFF3098 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.3.1.98), at 527,550 to 528,602
GFF3098
Position (kb) 526
527
528 Strain fitness (log2 ratio) -2
-1
0
1
2 at 526.612 kb on + strand at 526.613 kb on - strand at 526.870 kb on + strand, within GFF3097 at 526.870 kb on + strand, within GFF3097 at 526.871 kb on - strand, within GFF3097 at 526.896 kb on + strand, within GFF3097 at 526.896 kb on + strand, within GFF3097 at 526.896 kb on + strand, within GFF3097 at 526.896 kb on + strand, within GFF3097 at 526.896 kb on + strand, within GFF3097 at 526.896 kb on + strand, within GFF3097 at 526.896 kb on + strand, within GFF3097 at 526.896 kb on + strand, within GFF3097 at 526.896 kb on + strand, within GFF3097 at 526.897 kb on - strand, within GFF3097 at 526.897 kb on - strand, within GFF3097 at 526.897 kb on - strand, within GFF3097 at 526.926 kb on + strand, within GFF3097 at 526.926 kb on + strand, within GFF3097 at 526.926 kb on + strand, within GFF3097 at 526.926 kb on + strand, within GFF3097 at 526.926 kb on + strand, within GFF3097 at 526.926 kb on + strand, within GFF3097 at 526.926 kb on + strand, within GFF3097 at 526.926 kb on + strand, within GFF3097 at 526.927 kb on - strand, within GFF3097 at 526.927 kb on - strand, within GFF3097 at 526.927 kb on - strand, within GFF3097 at 526.927 kb on - strand, within GFF3097 at 527.028 kb on + strand, within GFF3097 at 527.028 kb on + strand, within GFF3097 at 527.029 kb on - strand, within GFF3097 at 527.075 kb on + strand, within GFF3097 at 527.075 kb on + strand, within GFF3097 at 527.076 kb on - strand, within GFF3097 at 527.076 kb on - strand, within GFF3097 at 527.076 kb on - strand, within GFF3097 at 527.511 kb on + strand at 527.511 kb on + strand at 527.511 kb on + strand at 527.511 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 526,612 + -0.2 526,613 - +0.2 526,870 + GFF3097 0.21 -1.3 526,870 + GFF3097 0.21 +0.1 526,871 - GFF3097 0.21 +0.1 526,896 + GFF3097 0.24 -1.0 526,896 + GFF3097 0.24 +0.4 526,896 + GFF3097 0.24 -0.6 526,896 + GFF3097 0.24 -0.4 526,896 + GFF3097 0.24 +0.4 526,896 + GFF3097 0.24 -0.8 526,896 + GFF3097 0.24 +0.1 526,896 + GFF3097 0.24 -0.1 526,896 + GFF3097 0.24 +0.4 526,897 - GFF3097 0.24 +0.1 526,897 - GFF3097 0.24 +2.3 526,897 - GFF3097 0.24 +0.7 526,926 + GFF3097 0.27 -0.1 526,926 + GFF3097 0.27 +0.4 526,926 + GFF3097 0.27 -0.9 526,926 + GFF3097 0.27 +0.2 526,926 + GFF3097 0.27 -0.0 526,926 + GFF3097 0.27 +0.5 526,926 + GFF3097 0.27 +0.4 526,926 + GFF3097 0.27 +0.2 526,927 - GFF3097 0.28 -1.3 526,927 - GFF3097 0.28 +0.2 526,927 - GFF3097 0.28 -0.3 526,927 - GFF3097 0.28 -1.6 527,028 + GFF3097 0.39 -0.7 527,028 + GFF3097 0.39 +0.8 527,029 - GFF3097 0.40 -1.0 527,075 + GFF3097 0.45 +0.4 527,075 + GFF3097 0.45 -0.1 527,076 - GFF3097 0.45 -0.9 527,076 - GFF3097 0.45 +1.1 527,076 - GFF3097 0.45 -1.9 527,511 + -0.3 527,511 + -0.1 527,511 + -0.3 527,511 + -0.7
Or see this region's nucleotide sequence