Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF289 and GFF290 are separated by 19 nucleotides GFF290 and GFF291 overlap by 1 nucleotides GFF291 and GFF292 are separated by 25 nucleotides
GFF289 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1, at 324,200 to 325,045
GFF289
GFF290 - Cell division coordinator CpoB, at 325,065 to 325,814
GFF290
GFF291 - Tol-Pal system peptidoglycan-associated lipoprotein PAL, at 325,814 to 326,332
GFF291
GFF292 - Tol-Pal system beta propeller repeat protein TolB, at 326,358 to 327,584
GFF292
Position (kb)
325
326 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 324.079 kb on - strand at 324.079 kb on - strand at 324.079 kb on - strand at 324.079 kb on - strand at 324.196 kb on - strand at 324.204 kb on - strand at 324.204 kb on - strand at 324.204 kb on - strand at 324.578 kb on + strand, within GFF289 at 324.578 kb on + strand, within GFF289 at 324.578 kb on + strand, within GFF289 at 324.578 kb on + strand, within GFF289 at 324.579 kb on - strand, within GFF289 at 324.579 kb on - strand, within GFF289 at 324.579 kb on - strand, within GFF289 at 324.579 kb on - strand, within GFF289 at 324.579 kb on - strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.934 kb on - strand, within GFF289 at 324.934 kb on - strand, within GFF289 at 324.934 kb on - strand, within GFF289 at 324.966 kb on + strand at 324.966 kb on + strand at 324.966 kb on + strand at 324.966 kb on + strand at 324.966 kb on + strand at 324.966 kb on + strand at 324.967 kb on - strand at 324.967 kb on - strand at 324.967 kb on - strand at 324.967 kb on - strand at 324.967 kb on - strand at 325.120 kb on + strand at 325.121 kb on - strand at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.288 kb on + strand, within GFF290 at 325.289 kb on - strand, within GFF290 at 325.327 kb on + strand, within GFF290 at 325.327 kb on + strand, within GFF290 at 325.327 kb on + strand, within GFF290 at 325.327 kb on + strand, within GFF290 at 325.328 kb on - strand, within GFF290 at 325.328 kb on - strand, within GFF290 at 325.342 kb on + strand, within GFF290 at 325.824 kb on + strand at 325.824 kb on + strand at 325.825 kb on - strand at 325.825 kb on - strand at 325.825 kb on - strand at 325.825 kb on - strand at 325.992 kb on + strand, within GFF291 at 325.992 kb on + strand, within GFF291 at 325.992 kb on + strand, within GFF291 at 325.992 kb on + strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.118 kb on + strand, within GFF291 at 326.127 kb on + strand, within GFF291 at 326.127 kb on + strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.313 kb on + strand at 326.321 kb on - strand at 326.349 kb on + strand at 326.349 kb on + strand at 326.349 kb on + strand at 326.350 kb on - strand at 326.355 kb on + strand at 326.355 kb on + strand at 326.355 kb on + strand at 326.355 kb on + strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.485 kb on + strand, within GFF292 at 326.486 kb on - strand, within GFF292 at 326.546 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.714 kb on - strand, within GFF292 at 326.714 kb on - strand, within GFF292 at 326.714 kb on - strand, within GFF292 at 326.714 kb on - strand, within GFF292 at 326.714 kb on - strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 324,079 - -0.6 324,079 - -0.0 324,079 - -1.2 324,079 - -0.4 324,196 - +0.1 324,204 - +0.5 324,204 - -1.6 324,204 - +0.4 324,578 + GFF289 0.45 -0.1 324,578 + GFF289 0.45 +0.6 324,578 + GFF289 0.45 -0.0 324,578 + GFF289 0.45 -0.1 324,579 - GFF289 0.45 -1.1 324,579 - GFF289 0.45 +1.4 324,579 - GFF289 0.45 +0.4 324,579 - GFF289 0.45 +0.5 324,579 - GFF289 0.45 -1.3 324,933 + GFF289 0.87 -0.7 324,933 + GFF289 0.87 -0.5 324,933 + GFF289 0.87 +0.3 324,933 + GFF289 0.87 +0.7 324,933 + GFF289 0.87 -0.4 324,933 + GFF289 0.87 +0.2 324,934 - GFF289 0.87 -0.4 324,934 - GFF289 0.87 -1.3 324,934 - GFF289 0.87 -0.5 324,966 + -0.3 324,966 + -0.2 324,966 + -0.3 324,966 + +1.7 324,966 + -2.8 324,966 + -0.4 324,967 - -0.1 324,967 - -0.6 324,967 - -3.5 324,967 - +0.4 324,967 - +0.0 325,120 + +1.0 325,121 - -1.3 325,273 + GFF290 0.28 -0.9 325,273 + GFF290 0.28 -0.9 325,273 + GFF290 0.28 -0.4 325,273 + GFF290 0.28 +3.0 325,273 + GFF290 0.28 -2.1 325,273 + GFF290 0.28 +0.4 325,273 + GFF290 0.28 +3.4 325,273 + GFF290 0.28 -0.5 325,273 + GFF290 0.28 -1.3 325,274 - GFF290 0.28 -0.3 325,274 - GFF290 0.28 -1.2 325,274 - GFF290 0.28 +1.4 325,274 - GFF290 0.28 -1.4 325,274 - GFF290 0.28 -1.4 325,274 - GFF290 0.28 -3.3 325,274 - GFF290 0.28 -1.8 325,274 - GFF290 0.28 -1.3 325,274 - GFF290 0.28 -0.3 325,274 - GFF290 0.28 +0.1 325,274 - GFF290 0.28 +1.1 325,288 + GFF290 0.30 -1.3 325,289 - GFF290 0.30 -1.6 325,327 + GFF290 0.35 -1.6 325,327 + GFF290 0.35 -0.7 325,327 + GFF290 0.35 -0.4 325,327 + GFF290 0.35 -1.0 325,328 - GFF290 0.35 -1.4 325,328 - GFF290 0.35 -1.8 325,342 + GFF290 0.37 -1.7 325,824 + -1.8 325,824 + -1.3 325,825 - -0.3 325,825 - -0.8 325,825 - +0.8 325,825 - -1.2 325,992 + GFF291 0.34 -2.0 325,992 + GFF291 0.34 +0.2 325,992 + GFF291 0.34 -2.1 325,992 + GFF291 0.34 -2.9 325,993 - GFF291 0.34 -1.4 325,993 - GFF291 0.34 -2.6 325,993 - GFF291 0.34 +0.6 325,993 - GFF291 0.34 -1.6 325,993 - GFF291 0.34 -2.9 325,993 - GFF291 0.34 +0.7 325,993 - GFF291 0.34 -4.0 325,993 - GFF291 0.34 -3.1 325,993 - GFF291 0.34 +1.4 325,993 - GFF291 0.34 -0.2 325,993 - GFF291 0.34 -1.4 325,993 - GFF291 0.34 -1.8 325,993 - GFF291 0.34 -2.6 325,993 - GFF291 0.34 -1.4 326,109 + GFF291 0.57 -3.9 326,109 + GFF291 0.57 -0.9 326,109 + GFF291 0.57 -0.6 326,109 + GFF291 0.57 -0.8 326,109 + GFF291 0.57 -2.1 326,109 + GFF291 0.57 -1.4 326,109 + GFF291 0.57 -1.9 326,109 + GFF291 0.57 -2.9 326,110 - GFF291 0.57 -2.5 326,110 - GFF291 0.57 -2.1 326,110 - GFF291 0.57 -2.3 326,110 - GFF291 0.57 -1.4 326,110 - GFF291 0.57 -0.6 326,110 - GFF291 0.57 -0.5 326,110 - GFF291 0.57 -2.0 326,110 - GFF291 0.57 -1.4 326,118 + GFF291 0.59 -1.4 326,127 + GFF291 0.60 -0.6 326,127 + GFF291 0.60 -2.3 326,128 - GFF291 0.61 -0.2 326,128 - GFF291 0.61 -1.3 326,128 - GFF291 0.61 -1.6 326,128 - GFF291 0.61 -1.7 326,128 - GFF291 0.61 -3.0 326,128 - GFF291 0.61 -2.8 326,128 - GFF291 0.61 -1.6 326,313 + -3.5 326,321 - -1.2 326,349 + +0.9 326,349 + -1.6 326,349 + +0.4 326,350 - -0.2 326,355 + +0.2 326,355 + -0.9 326,355 + -2.5 326,355 + -0.8 326,356 - -0.1 326,356 - -1.4 326,356 - +1.1 326,356 - +1.4 326,356 - -1.5 326,356 - +0.1 326,356 - -1.8 326,356 - -0.8 326,356 - -0.5 326,356 - -0.7 326,356 - -0.3 326,356 - +0.4 326,394 + -0.9 326,394 + +1.4 326,394 + -1.6 326,394 + -0.0 326,394 + -2.8 326,394 + -1.1 326,394 + -2.2 326,394 + -2.6 326,394 + -1.1 326,394 + -3.4 326,394 + -2.8 326,395 - -0.9 326,395 - -1.2 326,395 - -1.6 326,395 - -2.7 326,395 - -0.8 326,395 - -1.7 326,395 - -3.8 326,395 - -0.3 326,395 - -1.3 326,395 - -0.2 326,485 + GFF292 0.10 -0.2 326,486 - GFF292 0.10 -1.6 326,546 + GFF292 0.15 -1.8 326,614 + GFF292 0.21 -1.7 326,614 + GFF292 0.21 -0.9 326,614 + GFF292 0.21 -1.7 326,614 + GFF292 0.21 -0.9 326,614 + GFF292 0.21 -0.5 326,614 + GFF292 0.21 -1.4 326,614 + GFF292 0.21 -2.1 326,614 + GFF292 0.21 -2.1 326,614 + GFF292 0.21 -1.7 326,614 + GFF292 0.21 -0.3 326,615 - GFF292 0.21 -0.4 326,615 - GFF292 0.21 -3.6 326,615 - GFF292 0.21 -1.5 326,615 - GFF292 0.21 +1.8 326,615 - GFF292 0.21 -2.6 326,615 - GFF292 0.21 -1.5 326,615 - GFF292 0.21 -2.1 326,659 + GFF292 0.25 -1.6 326,659 + GFF292 0.25 -2.5 326,659 + GFF292 0.25 -3.4 326,659 + GFF292 0.25 -0.7 326,659 + GFF292 0.25 -0.6 326,659 + GFF292 0.25 -4.1 326,659 + GFF292 0.25 -1.0 326,659 + GFF292 0.25 -2.6 326,659 + GFF292 0.25 -2.4 326,659 + GFF292 0.25 -0.2 326,659 + GFF292 0.25 -2.5 326,660 - GFF292 0.25 +0.4 326,660 - GFF292 0.25 -1.9 326,660 - GFF292 0.25 -4.3 326,660 - GFF292 0.25 -1.2 326,660 - GFF292 0.25 +2.4 326,660 - GFF292 0.25 -1.4 326,660 - GFF292 0.25 -1.6 326,713 + GFF292 0.29 -2.4 326,713 + GFF292 0.29 -1.6 326,713 + GFF292 0.29 -2.9 326,713 + GFF292 0.29 +0.4 326,713 + GFF292 0.29 -2.8 326,713 + GFF292 0.29 -2.2 326,713 + GFF292 0.29 -1.4 326,713 + GFF292 0.29 -1.8 326,714 - GFF292 0.29 +1.4 326,714 - GFF292 0.29 -0.8 326,714 - GFF292 0.29 -0.9 326,714 - GFF292 0.29 -2.9 326,714 - GFF292 0.29 -1.2 326,749 + GFF292 0.32 -1.6 326,749 + GFF292 0.32 -0.7 326,749 + GFF292 0.32 +0.4 326,749 + GFF292 0.32 -2.6 326,749 + GFF292 0.32 -0.6 326,749 + GFF292 0.32 -1.8 326,749 + GFF292 0.32 -1.2 326,749 + GFF292 0.32 -2.7 326,749 + GFF292 0.32 -2.4 326,749 + GFF292 0.32 -0.9 326,749 + GFF292 0.32 -0.6 326,749 + GFF292 0.32 -1.4 326,750 - GFF292 0.32 +0.2 326,750 - GFF292 0.32 -1.1 326,750 - GFF292 0.32 -2.9 326,750 - GFF292 0.32 -1.9 326,750 - GFF292 0.32 -2.7 326,750 - GFF292 0.32 -2.2 326,750 - GFF292 0.32 -1.4 326,750 - GFF292 0.32 -3.1
Or see this region's nucleotide sequence