Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2792 and GFF2793 are separated by 109 nucleotides GFF2793 and GFF2794 overlap by 4 nucleotides GFF2794 and GFF2795 are separated by 27 nucleotides GFF2795 and GFF2796 are separated by 122 nucleotides
GFF2792 - Chaperone protein ClpB (ATP-dependent unfoldase), at 221,350 to 223,971
GFF2792
GFF2793 - Tricarboxylate transport protein TctC, at 224,081 to 225,073
GFF2793
GFF2794 - Molybdopterin synthase catalytic subunit MoaE (EC 2.8.1.12), at 225,070 to 225,546
GFF2794
GFF2795 - no description, at 225,574 to 225,687
GFF2795
GFF2796 - Dihydrodipicolinate synthase family protein PP2639, at 225,810 to 226,706
GFF2796
Position (kb) 224
225
226 Strain fitness (log2 ratio) -2
-1
0
1
2 at 223.321 kb on + strand, within GFF2792 at 223.322 kb on - strand, within GFF2792 at 223.335 kb on + strand, within GFF2792 at 223.487 kb on + strand, within GFF2792 at 223.487 kb on + strand, within GFF2792 at 223.487 kb on + strand, within GFF2792 at 223.488 kb on - strand, within GFF2792 at 223.889 kb on + strand at 223.889 kb on + strand at 223.889 kb on + strand at 223.889 kb on + strand at 223.889 kb on + strand at 223.889 kb on + strand at 223.889 kb on + strand at 223.890 kb on - strand at 223.890 kb on - strand at 223.890 kb on - strand at 223.890 kb on - strand at 223.890 kb on - strand at 223.890 kb on - strand at 223.995 kb on - strand at 223.995 kb on - strand at 224.389 kb on - strand, within GFF2793 at 224.391 kb on - strand, within GFF2793 at 224.522 kb on + strand, within GFF2793 at 224.522 kb on + strand, within GFF2793 at 224.523 kb on - strand, within GFF2793 at 224.523 kb on - strand, within GFF2793 at 224.523 kb on - strand, within GFF2793 at 224.523 kb on - strand, within GFF2793 at 224.523 kb on - strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.624 kb on + strand, within GFF2793 at 224.625 kb on - strand, within GFF2793 at 224.625 kb on - strand, within GFF2793 at 224.625 kb on - strand, within GFF2793 at 224.625 kb on - strand, within GFF2793 at 224.625 kb on - strand, within GFF2793 at 224.852 kb on + strand, within GFF2793 at 224.852 kb on + strand, within GFF2793 at 224.852 kb on + strand, within GFF2793 at 224.852 kb on + strand, within GFF2793 at 224.853 kb on - strand, within GFF2793 at 224.853 kb on - strand, within GFF2793 at 224.853 kb on - strand, within GFF2793 at 224.855 kb on + strand, within GFF2793 at 224.855 kb on + strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.856 kb on - strand, within GFF2793 at 224.903 kb on + strand, within GFF2793 at 224.903 kb on + strand, within GFF2793 at 224.903 kb on + strand, within GFF2793 at 224.903 kb on + strand, within GFF2793 at 224.904 kb on - strand, within GFF2793 at 224.904 kb on - strand, within GFF2793 at 224.904 kb on - strand, within GFF2793 at 224.981 kb on + strand at 224.982 kb on - strand at 224.982 kb on - strand at 225.020 kb on + strand at 225.020 kb on + strand at 225.020 kb on + strand at 225.020 kb on + strand at 225.020 kb on + strand at 225.021 kb on - strand at 225.021 kb on - strand at 225.021 kb on - strand at 225.071 kb on + strand at 225.071 kb on + strand at 225.071 kb on + strand at 225.072 kb on - strand at 225.072 kb on - strand at 225.072 kb on - strand at 225.072 kb on - strand at 225.072 kb on - strand at 225.072 kb on - strand at 225.194 kb on + strand, within GFF2794 at 225.194 kb on + strand, within GFF2794 at 225.194 kb on + strand, within GFF2794 at 225.194 kb on + strand, within GFF2794 at 225.195 kb on - strand, within GFF2794 at 225.195 kb on - strand, within GFF2794 at 225.195 kb on - strand, within GFF2794 at 225.381 kb on - strand, within GFF2794 at 225.381 kb on - strand, within GFF2794 at 225.568 kb on + strand at 225.569 kb on - strand at 225.569 kb on - strand at 225.834 kb on - strand at 225.834 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 223,321 + GFF2792 0.75 +0.7 223,322 - GFF2792 0.75 +0.3 223,335 + GFF2792 0.76 -1.7 223,487 + GFF2792 0.82 -2.5 223,487 + GFF2792 0.82 -0.9 223,487 + GFF2792 0.82 +0.5 223,488 - GFF2792 0.82 +0.1 223,889 + +0.2 223,889 + -0.3 223,889 + -1.7 223,889 + -1.5 223,889 + +1.4 223,889 + +0.7 223,889 + +1.2 223,890 - +0.4 223,890 - -1.3 223,890 - +1.2 223,890 - +0.5 223,890 - +0.4 223,890 - +0.7 223,995 - -1.0 223,995 - -0.1 224,389 - GFF2793 0.31 -0.1 224,391 - GFF2793 0.31 +0.4 224,522 + GFF2793 0.44 -0.1 224,522 + GFF2793 0.44 +1.2 224,523 - GFF2793 0.45 -0.8 224,523 - GFF2793 0.45 -1.8 224,523 - GFF2793 0.45 +0.3 224,523 - GFF2793 0.45 +0.8 224,523 - GFF2793 0.45 -1.1 224,624 + GFF2793 0.55 -1.1 224,624 + GFF2793 0.55 -0.1 224,624 + GFF2793 0.55 -0.0 224,624 + GFF2793 0.55 -0.7 224,624 + GFF2793 0.55 +0.7 224,624 + GFF2793 0.55 -1.3 224,624 + GFF2793 0.55 +0.4 224,624 + GFF2793 0.55 +0.0 224,624 + GFF2793 0.55 -0.3 224,624 + GFF2793 0.55 -0.5 224,624 + GFF2793 0.55 +0.1 224,625 - GFF2793 0.55 -0.8 224,625 - GFF2793 0.55 -0.5 224,625 - GFF2793 0.55 +0.2 224,625 - GFF2793 0.55 -2.0 224,625 - GFF2793 0.55 -0.3 224,852 + GFF2793 0.78 -0.1 224,852 + GFF2793 0.78 +0.9 224,852 + GFF2793 0.78 +1.2 224,852 + GFF2793 0.78 +0.3 224,853 - GFF2793 0.78 -0.5 224,853 - GFF2793 0.78 +0.1 224,853 - GFF2793 0.78 +2.1 224,855 + GFF2793 0.78 +0.1 224,855 + GFF2793 0.78 +0.1 224,856 - GFF2793 0.78 -0.2 224,856 - GFF2793 0.78 -0.7 224,856 - GFF2793 0.78 -0.3 224,856 - GFF2793 0.78 +0.7 224,856 - GFF2793 0.78 -0.2 224,856 - GFF2793 0.78 -0.5 224,856 - GFF2793 0.78 -0.1 224,903 + GFF2793 0.83 -0.6 224,903 + GFF2793 0.83 -1.4 224,903 + GFF2793 0.83 -1.1 224,903 + GFF2793 0.83 +0.2 224,904 - GFF2793 0.83 -0.3 224,904 - GFF2793 0.83 -0.7 224,904 - GFF2793 0.83 +0.8 224,981 + -0.8 224,982 - -0.2 224,982 - +1.1 225,020 + +1.1 225,020 + +0.2 225,020 + -0.1 225,020 + -1.0 225,020 + -0.5 225,021 - -0.3 225,021 - +0.6 225,021 - -0.5 225,071 + -0.3 225,071 + -0.1 225,071 + -0.4 225,072 - +0.5 225,072 - +1.2 225,072 - +0.5 225,072 - +0.0 225,072 - -0.9 225,072 - -1.2 225,194 + GFF2794 0.26 -0.6 225,194 + GFF2794 0.26 -0.3 225,194 + GFF2794 0.26 -0.6 225,194 + GFF2794 0.26 -0.7 225,195 - GFF2794 0.26 -0.3 225,195 - GFF2794 0.26 -0.7 225,195 - GFF2794 0.26 -0.2 225,381 - GFF2794 0.65 -0.5 225,381 - GFF2794 0.65 +0.7 225,568 + +0.8 225,569 - +2.1 225,569 - +0.6 225,834 - -0.8 225,834 - +0.5
Or see this region's nucleotide sequence