Strain Fitness in Variovorax sp. SCN45 around GFF273

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF271 and GFF272 overlap by 4 nucleotidesGFF272 and GFF273 are separated by 108 nucleotidesGFF273 and GFF274 are separated by 154 nucleotides GFF271 - 2-methylcitrate synthase (EC 2.3.3.5), at 307,217 to 308,380 GFF271 GFF272 - thioesterase superfamily protein, at 308,377 to 308,817 GFF272 GFF273 - Transcriptional regulator, Xre family, at 308,926 to 310,347 GFF273 GFF274 - Probable RNA methyltransferase PA1839, at 310,502 to 311,548 GFF274 Position (kb) 308 309 310 311Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 307.936 kb on - strand, within GFF271at 307.936 kb on - strand, within GFF271at 307.941 kb on + strand, within GFF271at 307.941 kb on + strand, within GFF271at 307.941 kb on + strand, within GFF271at 307.941 kb on + strand, within GFF271at 307.941 kb on + strand, within GFF271at 307.942 kb on - strand, within GFF271at 307.942 kb on - strand, within GFF271at 307.942 kb on - strand, within GFF271at 307.942 kb on - strand, within GFF271at 307.942 kb on - strand, within GFF271at 307.942 kb on - strand, within GFF271at 307.942 kb on - strand, within GFF271at 308.151 kb on + strand, within GFF271at 308.151 kb on + strand, within GFF271at 308.151 kb on + strand, within GFF271at 308.151 kb on + strand, within GFF271at 308.152 kb on - strand, within GFF271at 308.152 kb on - strand, within GFF271at 308.152 kb on - strand, within GFF271at 308.152 kb on - strand, within GFF271at 308.152 kb on - strand, within GFF271at 308.152 kb on - strand, within GFF271at 308.194 kb on + strand, within GFF271at 308.257 kb on - strand, within GFF271at 308.257 kb on - strand, within GFF271at 308.414 kb on + strandat 308.415 kb on - strandat 308.415 kb on - strandat 308.723 kb on + strand, within GFF272at 308.723 kb on + strand, within GFF272at 308.822 kb on + strandat 309.025 kb on + strandat 309.025 kb on + strandat 309.025 kb on + strandat 309.025 kb on + strandat 309.026 kb on - strandat 309.026 kb on - strandat 309.026 kb on - strandat 309.026 kb on - strandat 309.026 kb on - strandat 309.730 kb on + strand, within GFF273at 309.730 kb on + strand, within GFF273at 309.730 kb on + strand, within GFF273at 309.793 kb on + strand, within GFF273at 309.794 kb on - strand, within GFF273at 309.997 kb on + strand, within GFF273at 309.997 kb on + strand, within GFF273at 309.997 kb on + strand, within GFF273at 309.998 kb on - strand, within GFF273at 309.998 kb on - strand, within GFF273at 309.998 kb on - strand, within GFF273at 309.998 kb on - strand, within GFF273at 310.063 kb on + strand, within GFF273at 310.063 kb on + strand, within GFF273at 310.063 kb on + strand, within GFF273at 310.063 kb on + strand, within GFF273at 310.063 kb on + strand, within GFF273at 310.063 kb on + strand, within GFF273at 310.063 kb on + strand, within GFF273at 310.063 kb on + strand, within GFF273at 310.063 kb on + strand, within GFF273at 310.063 kb on + strand, within GFF273at 310.064 kb on - strand, within GFF273at 310.064 kb on - strand, within GFF273at 310.064 kb on - strand, within GFF273at 310.064 kb on - strand, within GFF273at 310.064 kb on - strand, within GFF273at 310.064 kb on - strand, within GFF273at 310.064 kb on - strand, within GFF273at 310.174 kb on + strand, within GFF273at 310.174 kb on + strand, within GFF273at 310.174 kb on + strand, within GFF273at 310.174 kb on + strand, within GFF273at 310.174 kb on + strand, within GFF273at 310.174 kb on + strand, within GFF273at 310.174 kb on + strand, within GFF273at 310.174 kb on + strand, within GFF273at 310.175 kb on - strand, within GFF273at 310.175 kb on - strand, within GFF273at 310.175 kb on - strand, within GFF273at 310.175 kb on - strand, within GFF273at 310.175 kb on - strand, within GFF273at 310.327 kb on + strandat 310.327 kb on + strandat 310.327 kb on + strandat 310.327 kb on + strandat 310.327 kb on + strandat 310.328 kb on - strandat 310.503 kb on + strandat 310.503 kb on + strandat 310.503 kb on + strandat 310.503 kb on + strandat 310.503 kb on + strandat 310.503 kb on + strandat 310.503 kb on + strandat 310.504 kb on - strandat 310.504 kb on - strandat 310.504 kb on - strandat 310.504 kb on - strandat 310.504 kb on - strandat 310.504 kb on - strandat 310.504 kb on - strandat 310.737 kb on + strand, within GFF274at 310.737 kb on + strand, within GFF274at 310.737 kb on + strand, within GFF274at 310.737 kb on + strand, within GFF274at 310.737 kb on + strand, within GFF274at 310.738 kb on - strand, within GFF274at 310.815 kb on + strand, within GFF274at 310.816 kb on - strand, within GFF274at 310.845 kb on + strand, within GFF274at 310.846 kb on - strand, within GFF274

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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307,936 - GFF271 0.62 -0.9
307,936 - GFF271 0.62 +0.7
307,941 + GFF271 0.62 -0.4
307,941 + GFF271 0.62 -0.2
307,941 + GFF271 0.62 -1.0
307,941 + GFF271 0.62 -1.9
307,941 + GFF271 0.62 +0.8
307,942 - GFF271 0.62 +1.7
307,942 - GFF271 0.62 -1.1
307,942 - GFF271 0.62 -1.2
307,942 - GFF271 0.62 -0.2
307,942 - GFF271 0.62 -2.2
307,942 - GFF271 0.62 -2.1
307,942 - GFF271 0.62 +0.8
308,151 + GFF271 0.80 -1.8
308,151 + GFF271 0.80 -0.2
308,151 + GFF271 0.80 -0.8
308,151 + GFF271 0.80 +0.2
308,152 - GFF271 0.80 +0.4
308,152 - GFF271 0.80 -0.9
308,152 - GFF271 0.80 -0.9
308,152 - GFF271 0.80 -0.7
308,152 - GFF271 0.80 +0.2
308,152 - GFF271 0.80 -1.4
308,194 + GFF271 0.84 -0.3
308,257 - GFF271 0.89 +1.6
308,257 - GFF271 0.89 -0.7
308,414 + -1.8
308,415 - -0.2
308,415 - +1.4
308,723 + GFF272 0.78 -1.7
308,723 + GFF272 0.78 +0.9
308,822 + -0.8
309,025 + -0.9
309,025 + +0.4
309,025 + -0.4
309,025 + -0.7
309,026 - -2.3
309,026 - +0.2
309,026 - -2.5
309,026 - -1.9
309,026 - -1.2
309,730 + GFF273 0.57 +0.4
309,730 + GFF273 0.57 -1.2
309,730 + GFF273 0.57 -1.2
309,793 + GFF273 0.61 -1.4
309,794 - GFF273 0.61 -1.2
309,997 + GFF273 0.75 -1.1
309,997 + GFF273 0.75 -0.2
309,997 + GFF273 0.75 +0.4
309,998 - GFF273 0.75 -0.9
309,998 - GFF273 0.75 -2.3
309,998 - GFF273 0.75 -0.0
309,998 - GFF273 0.75 -0.9
310,063 + GFF273 0.80 -0.6
310,063 + GFF273 0.80 -1.2
310,063 + GFF273 0.80 -0.6
310,063 + GFF273 0.80 -1.4
310,063 + GFF273 0.80 -0.7
310,063 + GFF273 0.80 -1.4
310,063 + GFF273 0.80 -0.6
310,063 + GFF273 0.80 -1.2
310,063 + GFF273 0.80 -1.5
310,063 + GFF273 0.80 +1.4
310,064 - GFF273 0.80 -1.3
310,064 - GFF273 0.80 -2.9
310,064 - GFF273 0.80 -1.4
310,064 - GFF273 0.80 -2.1
310,064 - GFF273 0.80 +1.0
310,064 - GFF273 0.80 -0.3
310,064 - GFF273 0.80 -0.2
310,174 + GFF273 0.88 -1.9
310,174 + GFF273 0.88 +0.2
310,174 + GFF273 0.88 +0.4
310,174 + GFF273 0.88 -2.0
310,174 + GFF273 0.88 -0.3
310,174 + GFF273 0.88 +0.5
310,174 + GFF273 0.88 +0.8
310,174 + GFF273 0.88 -1.9
310,175 - GFF273 0.88 -0.9
310,175 - GFF273 0.88 -0.5
310,175 - GFF273 0.88 -0.8
310,175 - GFF273 0.88 -1.4
310,175 - GFF273 0.88 +1.4
310,327 + -1.1
310,327 + -0.7
310,327 + -1.6
310,327 + -0.9
310,327 + -1.7
310,328 - -0.4
310,503 + +0.8
310,503 + +0.0
310,503 + +0.4
310,503 + -0.4
310,503 + +1.1
310,503 + +1.7
310,503 + -0.6
310,504 - +0.1
310,504 - +0.5
310,504 - -0.8
310,504 - -1.1
310,504 - -0.6
310,504 - -0.4
310,504 - -0.6
310,737 + GFF274 0.22 -1.8
310,737 + GFF274 0.22 -2.1
310,737 + GFF274 0.22 +0.4
310,737 + GFF274 0.22 -0.1
310,737 + GFF274 0.22 -0.3
310,738 - GFF274 0.23 +1.4
310,815 + GFF274 0.30 +2.1
310,816 - GFF274 0.30 -0.4
310,845 + GFF274 0.33 -0.8
310,846 - GFF274 0.33 +0.7

Or see this region's nucleotide sequence