Strain Fitness in Variovorax sp. SCN45 around GFF2698

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2696 and GFF2697 overlap by 1 nucleotidesGFF2697 and GFF2698 are separated by 49 nucleotidesGFF2698 and GFF2699 are separated by 27 nucleotidesGFF2699 and GFF2700 are separated by 28 nucleotides GFF2696 - L-2-keto-3-deoxyarabonate dehydratase (EC 4.2.1.43), at 124,563 to 125,501 GFF2696 GFF2697 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 125,501 to 126,262 GFF2697 GFF2698 - Transcriptional regulator, LysR family, at 126,312 to 127,226 GFF2698 GFF2699 - 4Fe-4S ferredoxin, iron-sulfur binding, at 127,254 to 127,589 GFF2699 GFF2700 - Enoyl-CoA hydratase (EC 4.2.1.17), at 127,618 to 128,394 GFF2700 Position (kb) 126 127 128Strain fitness (log2 ratio) -2 -1 0 1 2at 125.791 kb on - strand, within GFF2697at 125.791 kb on - strand, within GFF2697at 125.940 kb on + strand, within GFF2697at 125.940 kb on + strand, within GFF2697at 125.941 kb on - strand, within GFF2697at 126.862 kb on + strand, within GFF2698at 126.862 kb on + strand, within GFF2698at 126.862 kb on + strand, within GFF2698at 126.863 kb on - strand, within GFF2698at 126.863 kb on - strand, within GFF2698at 127.204 kb on + strandat 127.423 kb on + strand, within GFF2699at 127.489 kb on + strand, within GFF2699at 127.511 kb on - strand, within GFF2699at 127.511 kb on - strand, within GFF2699at 127.597 kb on - strandat 127.620 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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125,791 - GFF2697 0.38 +2.4
125,791 - GFF2697 0.38 -1.1
125,940 + GFF2697 0.58 +0.7
125,940 + GFF2697 0.58 -0.5
125,941 - GFF2697 0.58 +0.1
126,862 + GFF2698 0.60 -0.5
126,862 + GFF2698 0.60 -1.9
126,862 + GFF2698 0.60 -0.7
126,863 - GFF2698 0.60 -0.7
126,863 - GFF2698 0.60 -1.7
127,204 + -1.8
127,423 + GFF2699 0.50 -1.9
127,489 + GFF2699 0.70 +1.7
127,511 - GFF2699 0.76 -0.3
127,511 - GFF2699 0.76 -0.7
127,597 - +1.5
127,620 - +0.6

Or see this region's nucleotide sequence