Strain Fitness in Variovorax sp. SCN45 around GFF26

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF25 and GFF26 are separated by 134 nucleotidesGFF26 and GFF27 overlap by 4 nucleotidesGFF27 and GFF28 are separated by 14 nucleotides GFF25 - Alcohol dehydrogenase (EC 1.1.1.1), at 29,212 to 30,348 GFF25 GFF26 - Esterase/lipase, at 30,483 to 31,463 GFF26 GFF27 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 31,460 to 32,335 GFF27 GFF28 - hypothetical protein, at 32,350 to 33,246 GFF28 Position (kb) 30 31 32Strain fitness (log2 ratio) -3 -2 -1 0 1at 30.003 kb on - strand, within GFF25at 30.003 kb on - strand, within GFF25at 30.003 kb on - strand, within GFF25at 30.003 kb on - strand, within GFF25at 30.003 kb on - strand, within GFF25at 30.110 kb on + strand, within GFF25at 30.111 kb on - strand, within GFF25at 30.111 kb on - strand, within GFF25at 30.332 kb on + strandat 30.333 kb on - strandat 30.333 kb on - strandat 30.394 kb on + strandat 30.395 kb on - strandat 30.476 kb on + strandat 30.631 kb on - strand, within GFF26at 30.735 kb on + strand, within GFF26at 30.735 kb on + strand, within GFF26at 30.771 kb on + strand, within GFF26at 31.080 kb on + strand, within GFF26at 31.081 kb on - strand, within GFF26at 31.081 kb on - strand, within GFF26at 31.179 kb on + strand, within GFF26at 31.179 kb on + strand, within GFF26at 31.180 kb on - strand, within GFF26at 31.180 kb on - strand, within GFF26at 31.180 kb on - strand, within GFF26at 31.180 kb on - strand, within GFF26at 31.309 kb on - strand, within GFF26at 31.356 kb on + strand, within GFF26at 31.562 kb on + strand, within GFF27at 31.562 kb on + strand, within GFF27at 31.563 kb on - strand, within GFF27at 31.563 kb on - strand, within GFF27at 31.563 kb on - strand, within GFF27at 31.563 kb on - strand, within GFF27at 31.563 kb on - strand, within GFF27at 31.563 kb on - strand, within GFF27at 31.563 kb on - strand, within GFF27at 31.571 kb on + strand, within GFF27at 31.595 kb on + strand, within GFF27at 31.619 kb on + strand, within GFF27at 31.620 kb on - strand, within GFF27at 31.655 kb on + strand, within GFF27at 31.655 kb on + strand, within GFF27at 31.656 kb on - strand, within GFF27at 31.811 kb on + strand, within GFF27at 31.811 kb on + strand, within GFF27at 31.811 kb on + strand, within GFF27at 31.811 kb on + strand, within GFF27at 31.811 kb on + strand, within GFF27at 31.811 kb on + strand, within GFF27at 31.811 kb on + strand, within GFF27at 31.812 kb on - strand, within GFF27at 31.812 kb on - strand, within GFF27at 31.812 kb on - strand, within GFF27at 31.812 kb on - strand, within GFF27at 31.812 kb on - strand, within GFF27at 31.883 kb on + strand, within GFF27at 31.883 kb on + strand, within GFF27at 32.249 kb on + strandat 32.249 kb on + strandat 32.249 kb on + strandat 32.249 kb on + strandat 32.250 kb on - strandat 32.250 kb on - strandat 32.250 kb on - strandat 32.250 kb on - strandat 32.250 kb on - strandat 32.264 kb on + strandat 32.264 kb on + strandat 32.264 kb on + strandat 32.265 kb on - strandat 32.379 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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30,003 - GFF25 0.70 +0.3
30,003 - GFF25 0.70 +0.2
30,003 - GFF25 0.70 +0.9
30,003 - GFF25 0.70 -0.1
30,003 - GFF25 0.70 -0.5
30,110 + GFF25 0.79 -0.0
30,111 - GFF25 0.79 +0.5
30,111 - GFF25 0.79 -0.6
30,332 + -0.6
30,333 - +0.4
30,333 - -0.5
30,394 + -1.0
30,395 - +1.0
30,476 + -1.2
30,631 - GFF26 0.15 -0.4
30,735 + GFF26 0.26 -1.6
30,735 + GFF26 0.26 -3.0
30,771 + GFF26 0.29 -0.9
31,080 + GFF26 0.61 -0.4
31,081 - GFF26 0.61 +0.4
31,081 - GFF26 0.61 -0.4
31,179 + GFF26 0.71 -0.5
31,179 + GFF26 0.71 -0.3
31,180 - GFF26 0.71 +1.5
31,180 - GFF26 0.71 +0.0
31,180 - GFF26 0.71 -0.4
31,180 - GFF26 0.71 +0.6
31,309 - GFF26 0.84 -0.5
31,356 + GFF26 0.89 -0.2
31,562 + GFF27 0.12 +1.1
31,562 + GFF27 0.12 -0.0
31,563 - GFF27 0.12 +0.6
31,563 - GFF27 0.12 +0.0
31,563 - GFF27 0.12 -0.3
31,563 - GFF27 0.12 -1.7
31,563 - GFF27 0.12 +0.4
31,563 - GFF27 0.12 +0.8
31,563 - GFF27 0.12 -0.4
31,571 + GFF27 0.13 +0.6
31,595 + GFF27 0.15 -1.6
31,619 + GFF27 0.18 -0.8
31,620 - GFF27 0.18 -1.6
31,655 + GFF27 0.22 -0.7
31,655 + GFF27 0.22 +0.6
31,656 - GFF27 0.22 +1.4
31,811 + GFF27 0.40 -0.2
31,811 + GFF27 0.40 -2.2
31,811 + GFF27 0.40 -0.5
31,811 + GFF27 0.40 -0.6
31,811 + GFF27 0.40 +1.4
31,811 + GFF27 0.40 +1.2
31,811 + GFF27 0.40 -0.4
31,812 - GFF27 0.40 -0.0
31,812 - GFF27 0.40 -0.5
31,812 - GFF27 0.40 +0.3
31,812 - GFF27 0.40 +0.0
31,812 - GFF27 0.40 -0.1
31,883 + GFF27 0.48 -0.0
31,883 + GFF27 0.48 +0.5
32,249 + -0.1
32,249 + +0.5
32,249 + -0.5
32,249 + +1.6
32,250 - +0.0
32,250 - -0.7
32,250 - +1.3
32,250 - +1.4
32,250 - +0.8
32,264 + -2.5
32,264 + -0.5
32,264 + -0.8
32,265 - -0.3
32,379 - -0.1

Or see this region's nucleotide sequence