Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2596 and GFF2597 overlap by 4 nucleotides GFF2597 and GFF2598 overlap by 4 nucleotides GFF2598 and GFF2599 are separated by 16 nucleotides GFF2599 and GFF2600 are separated by 28 nucleotides
GFF2596 - Multidrug resistance transporter, Bcr/CflA family, at 1,931 to 3,151
GFF2596
GFF2597 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 3,148 to 3,582
GFF2597
GFF2598 - 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2), at 3,579 to 4,793
GFF2598
GFF2599 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 4,810 to 5,643
GFF2599
GFF2600 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 5,672 to 6,595
GFF2600
Position (kb) 3
4
5 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 2.604 kb on + strand, within GFF2596 at 2.604 kb on + strand, within GFF2596 at 2.605 kb on - strand, within GFF2596 at 2.634 kb on + strand, within GFF2596 at 2.634 kb on + strand, within GFF2596 at 2.635 kb on - strand, within GFF2596 at 2.635 kb on - strand, within GFF2596 at 2.635 kb on - strand, within GFF2596 at 2.635 kb on - strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 3.054 kb on + strand at 3.054 kb on + strand at 3.054 kb on + strand at 3.054 kb on + strand at 3.054 kb on + strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.248 kb on + strand, within GFF2597 at 3.248 kb on + strand, within GFF2597 at 3.248 kb on + strand, within GFF2597 at 3.249 kb on - strand, within GFF2597 at 3.386 kb on + strand, within GFF2597 at 3.386 kb on + strand, within GFF2597 at 3.387 kb on - strand, within GFF2597 at 3.387 kb on - strand, within GFF2597 at 3.551 kb on + strand at 3.551 kb on + strand at 3.551 kb on + strand at 3.551 kb on + strand at 3.552 kb on - strand at 3.552 kb on - strand at 3.552 kb on - strand at 4.022 kb on - strand, within GFF2598 at 4.022 kb on - strand, within GFF2598 at 4.022 kb on - strand, within GFF2598 at 4.022 kb on - strand, within GFF2598 at 4.022 kb on - strand, within GFF2598 at 4.504 kb on + strand, within GFF2598 at 4.504 kb on + strand, within GFF2598 at 4.505 kb on - strand, within GFF2598 at 4.505 kb on - strand, within GFF2598 at 4.505 kb on - strand, within GFF2598 at 4.787 kb on + strand at 4.788 kb on - strand at 4.788 kb on - strand at 4.814 kb on + strand at 4.815 kb on - strand at 4.815 kb on - strand at 4.837 kb on + strand at 4.837 kb on + strand at 4.837 kb on + strand at 4.837 kb on + strand at 4.837 kb on + strand at 4.837 kb on + strand at 4.837 kb on + strand at 4.837 kb on + strand at 4.838 kb on - strand at 4.838 kb on - strand at 4.838 kb on - strand at 4.838 kb on - strand at 4.838 kb on - strand at 4.880 kb on + strand at 4.881 kb on - strand at 4.964 kb on + strand, within GFF2599 at 4.964 kb on + strand, within GFF2599 at 4.965 kb on - strand, within GFF2599 at 4.965 kb on - strand, within GFF2599 at 5.081 kb on + strand, within GFF2599 at 5.126 kb on + strand, within GFF2599 at 5.126 kb on + strand, within GFF2599 at 5.126 kb on + strand, within GFF2599 at 5.501 kb on + strand, within GFF2599 at 5.502 kb on - strand, within GFF2599 at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.570 kb on + strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.571 kb on - strand at 5.693 kb on - strand at 5.695 kb on - strand at 5.737 kb on - strand at 5.737 kb on - strand at 5.737 kb on - strand at 5.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 2,604 + GFF2596 0.55 -1.2 2,604 + GFF2596 0.55 +0.1 2,605 - GFF2596 0.55 +1.1 2,634 + GFF2596 0.58 -2.3 2,634 + GFF2596 0.58 +0.7 2,635 - GFF2596 0.58 +0.1 2,635 - GFF2596 0.58 +0.1 2,635 - GFF2596 0.58 +0.5 2,635 - GFF2596 0.58 +0.6 2,898 + GFF2596 0.79 -0.1 2,898 + GFF2596 0.79 +0.7 2,898 + GFF2596 0.79 +0.4 2,898 + GFF2596 0.79 -0.6 2,898 + GFF2596 0.79 +0.2 2,898 + GFF2596 0.79 -1.1 2,898 + GFF2596 0.79 -0.4 2,899 - GFF2596 0.79 +0.3 2,899 - GFF2596 0.79 +2.7 2,899 - GFF2596 0.79 +0.6 2,899 - GFF2596 0.79 -0.5 2,899 - GFF2596 0.79 +2.2 2,899 - GFF2596 0.79 -0.5 2,899 - GFF2596 0.79 -1.8 2,899 - GFF2596 0.79 -0.8 2,899 - GFF2596 0.79 +3.0 2,899 - GFF2596 0.79 +0.4 2,899 - GFF2596 0.79 -0.4 3,054 + -0.7 3,054 + -0.5 3,054 + -0.4 3,054 + -0.4 3,054 + -0.3 3,055 - +0.4 3,055 - +0.2 3,055 - +2.1 3,055 - -0.4 3,055 - -0.4 3,055 - -0.1 3,055 - +0.4 3,248 + GFF2597 0.23 -0.6 3,248 + GFF2597 0.23 +0.1 3,248 + GFF2597 0.23 +1.7 3,249 - GFF2597 0.23 +0.4 3,386 + GFF2597 0.55 -0.1 3,386 + GFF2597 0.55 -0.3 3,387 - GFF2597 0.55 +0.1 3,387 - GFF2597 0.55 -0.1 3,551 + -0.7 3,551 + +0.5 3,551 + -1.0 3,551 + +0.4 3,552 - -0.9 3,552 - -1.1 3,552 - -0.0 4,022 - GFF2598 0.36 -1.8 4,022 - GFF2598 0.36 +0.2 4,022 - GFF2598 0.36 +0.1 4,022 - GFF2598 0.36 +0.1 4,022 - GFF2598 0.36 -0.4 4,504 + GFF2598 0.76 -0.9 4,504 + GFF2598 0.76 -0.6 4,505 - GFF2598 0.76 -0.8 4,505 - GFF2598 0.76 -0.1 4,505 - GFF2598 0.76 +0.1 4,787 + +0.7 4,788 - +0.1 4,788 - -0.5 4,814 + +1.9 4,815 - -1.3 4,815 - +0.8 4,837 + +1.4 4,837 + -0.4 4,837 + +0.5 4,837 + -0.1 4,837 + -0.8 4,837 + -0.1 4,837 + -0.6 4,837 + +1.0 4,838 - +0.1 4,838 - -1.5 4,838 - -2.9 4,838 - +2.7 4,838 - +0.5 4,880 + -1.0 4,881 - -0.1 4,964 + GFF2599 0.18 -0.8 4,964 + GFF2599 0.18 +0.1 4,965 - GFF2599 0.19 -0.7 4,965 - GFF2599 0.19 -0.9 5,081 + GFF2599 0.32 -0.7 5,126 + GFF2599 0.38 -1.1 5,126 + GFF2599 0.38 +0.2 5,126 + GFF2599 0.38 +0.6 5,501 + GFF2599 0.83 +0.1 5,502 - GFF2599 0.83 -1.3 5,570 + -1.8 5,570 + +0.4 5,570 + -0.1 5,570 + +0.1 5,570 + +0.9 5,570 + -0.1 5,570 + -0.4 5,570 + -0.4 5,570 + +0.7 5,570 + -2.0 5,570 + -0.1 5,570 + -0.7 5,570 + -0.4 5,571 - +0.3 5,571 - +0.1 5,571 - -0.0 5,571 - +0.1 5,571 - -0.0 5,571 - +1.3 5,571 - -0.0 5,571 - -1.0 5,571 - +0.2 5,571 - +0.2 5,571 - +1.4 5,571 - +0.0 5,571 - +0.5 5,693 - +0.1 5,695 - -0.8 5,737 - +0.3 5,737 - -0.8 5,737 - +0.3 5,737 - -2.1
Or see this region's nucleotide sequence