Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2576 and GFF2577 are separated by 40 nucleotides GFF2577 and GFF2578 overlap by 4 nucleotides GFF2578 and GFF2579 are separated by 19 nucleotides GFF2579 and GFF2580 are separated by 69 nucleotides
GFF2576 - 6-carboxy-5,6,7,8-tetrahydropterin synthase (EC 4.1.2.50), at 633,652 to 634,014
GFF2576
GFF2577 - 7-carboxy-7-deazaguanine synthase (EC 4.3.99.3), at 634,055 to 634,693
GFF2577
GFF2578 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 634,690 to 635,532
GFF2578
GFF2579 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 635,552 to 636,445
GFF2579
GFF2580 - L-aspartate oxidase (EC 1.4.3.16), at 636,515 to 638,101
GFF2580
Position (kb) 634
635
636 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.930 kb on + strand, within GFF2576 at 633.930 kb on + strand, within GFF2576 at 633.931 kb on - strand, within GFF2576 at 634.665 kb on + strand at 634.665 kb on + strand at 634.665 kb on + strand at 634.665 kb on + strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.686 kb on + strand at 634.686 kb on + strand at 634.686 kb on + strand at 634.686 kb on + strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.823 kb on + strand, within GFF2578 at 634.823 kb on + strand, within GFF2578 at 634.823 kb on + strand, within GFF2578 at 634.823 kb on + strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.873 kb on + strand, within GFF2578 at 634.874 kb on - strand, within GFF2578 at 634.874 kb on - strand, within GFF2578 at 634.874 kb on - strand, within GFF2578 at 634.973 kb on + strand, within GFF2578 at 634.974 kb on - strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.554 kb on - strand at 635.554 kb on - strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 636.084 kb on + strand, within GFF2579 at 636.084 kb on + strand, within GFF2579 at 636.085 kb on - strand, within GFF2579 at 636.165 kb on + strand, within GFF2579 at 636.165 kb on + strand, within GFF2579 at 636.165 kb on + strand, within GFF2579 at 636.166 kb on - strand, within GFF2579 at 636.291 kb on + strand, within GFF2579 at 636.291 kb on + strand, within GFF2579 at 636.292 kb on - strand, within GFF2579 at 636.292 kb on - strand, within GFF2579 at 636.292 kb on - strand, within GFF2579 at 636.399 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 633,914 + GFF2576 0.72 -0.7 633,914 + GFF2576 0.72 -0.9 633,914 + GFF2576 0.72 -0.4 633,914 + GFF2576 0.72 -0.1 633,914 + GFF2576 0.72 -0.4 633,914 + GFF2576 0.72 -0.2 633,914 + GFF2576 0.72 -0.2 633,914 + GFF2576 0.72 +1.4 633,914 + GFF2576 0.72 +0.2 633,915 - GFF2576 0.72 +1.5 633,915 - GFF2576 0.72 -1.2 633,915 - GFF2576 0.72 -1.8 633,915 - GFF2576 0.72 +0.8 633,915 - GFF2576 0.72 -2.6 633,930 + GFF2576 0.77 -0.2 633,930 + GFF2576 0.77 -1.0 633,931 - GFF2576 0.77 -0.1 634,665 + +0.7 634,665 + +0.1 634,665 + +2.5 634,665 + -0.3 634,666 - -0.9 634,666 - +0.1 634,666 - -1.2 634,666 - -1.7 634,666 - -0.7 634,666 - -1.1 634,666 - -0.8 634,666 - -0.1 634,666 - +0.2 634,666 - +1.0 634,686 + +1.5 634,686 + -0.6 634,686 + -0.5 634,686 + -1.0 634,687 - +0.2 634,687 - +1.2 634,687 - +0.0 634,687 - -0.3 634,687 - -2.0 634,687 - -1.2 634,687 - -0.9 634,687 - +1.5 634,687 - -0.7 634,823 + GFF2578 0.16 -1.8 634,823 + GFF2578 0.16 -1.7 634,823 + GFF2578 0.16 -1.1 634,823 + GFF2578 0.16 -2.5 634,824 - GFF2578 0.16 -1.9 634,824 - GFF2578 0.16 -4.2 634,824 - GFF2578 0.16 -1.9 634,824 - GFF2578 0.16 -1.4 634,824 - GFF2578 0.16 -1.4 634,824 - GFF2578 0.16 -1.2 634,873 + GFF2578 0.22 -2.1 634,874 - GFF2578 0.22 -2.0 634,874 - GFF2578 0.22 -2.1 634,874 - GFF2578 0.22 -3.4 634,973 + GFF2578 0.34 +0.6 634,974 - GFF2578 0.34 -1.9 635,090 + GFF2578 0.47 -1.6 635,090 + GFF2578 0.47 -2.6 635,090 + GFF2578 0.47 -1.8 635,090 + GFF2578 0.47 -1.3 635,090 + GFF2578 0.47 -2.0 635,091 - GFF2578 0.48 -1.0 635,091 - GFF2578 0.48 -1.0 635,091 - GFF2578 0.48 -1.8 635,091 - GFF2578 0.48 -3.0 635,091 - GFF2578 0.48 -2.4 635,334 - GFF2578 0.76 -1.0 635,334 - GFF2578 0.76 -1.6 635,334 - GFF2578 0.76 -0.8 635,334 - GFF2578 0.76 -1.8 635,528 + -2.2 635,528 + -0.8 635,528 + -2.4 635,528 + -1.8 635,528 + -0.9 635,528 + -2.1 635,528 + -1.4 635,528 + -1.6 635,528 + -0.4 635,528 + -3.1 635,529 - -1.6 635,529 - -1.5 635,529 - -0.4 635,529 - +0.8 635,529 - -3.4 635,529 - -2.2 635,529 - -0.4 635,529 - -2.4 635,554 - -1.0 635,554 - -1.9 635,607 + +0.0 635,607 + +0.6 635,607 + -0.5 635,607 + -1.7 635,607 + -0.7 635,607 + +0.2 635,607 + +0.0 635,607 + +0.7 635,607 + -0.6 635,607 + +0.9 635,607 + -0.8 635,607 + -0.1 635,608 - +0.1 635,608 - +1.2 635,608 - +0.3 635,608 - +0.2 635,608 - +0.8 635,608 - +0.6 635,608 - +0.3 635,608 - +0.6 635,608 - +0.3 635,918 + GFF2579 0.41 +0.9 635,918 + GFF2579 0.41 -1.5 635,918 + GFF2579 0.41 -0.2 635,918 + GFF2579 0.41 +1.3 635,918 + GFF2579 0.41 -0.4 635,918 + GFF2579 0.41 -1.7 635,918 + GFF2579 0.41 -1.8 635,918 + GFF2579 0.41 +1.4 635,918 + GFF2579 0.41 -0.1 635,919 - GFF2579 0.41 -1.4 635,919 - GFF2579 0.41 -1.0 635,919 - GFF2579 0.41 -1.5 635,919 - GFF2579 0.41 +0.9 635,919 - GFF2579 0.41 +0.4 635,919 - GFF2579 0.41 +0.6 635,919 - GFF2579 0.41 -0.8 635,919 - GFF2579 0.41 -0.8 635,919 - GFF2579 0.41 +0.2 635,919 - GFF2579 0.41 +0.4 635,919 - GFF2579 0.41 +0.1 635,919 - GFF2579 0.41 +0.8 635,919 - GFF2579 0.41 +0.2 636,084 + GFF2579 0.60 +1.4 636,084 + GFF2579 0.60 +1.7 636,085 - GFF2579 0.60 -0.0 636,165 + GFF2579 0.69 +2.0 636,165 + GFF2579 0.69 +0.2 636,165 + GFF2579 0.69 -0.7 636,166 - GFF2579 0.69 +0.0 636,291 + GFF2579 0.83 +1.0 636,291 + GFF2579 0.83 -0.1 636,292 - GFF2579 0.83 +0.5 636,292 - GFF2579 0.83 -0.6 636,292 - GFF2579 0.83 +0.6 636,399 + -0.0
Or see this region's nucleotide sequence