Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2422 and GFF2423 are separated by 36 nucleotides GFF2423 and GFF2424 overlap by 4 nucleotides GFF2424 and GFF2425 overlap by 4 nucleotides
GFF2422 - Polysaccharide deacetylase, at 473,394 to 474,218
GFF2422
GFF2423 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 474,255 to 475,046
GFF2423
GFF2424 - ABC transporter, permease protein 1 (cluster 1, maltose/g3p/polyamine/iron), at 475,043 to 475,894
GFF2424
GFF2425 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 475,891 to 476,982
GFF2425
Position (kb) 475
476 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.604 kb on - strand, within GFF2423 at 474.607 kb on + strand, within GFF2423 at 474.608 kb on - strand, within GFF2423 at 474.608 kb on - strand, within GFF2423 at 474.676 kb on + strand, within GFF2423 at 474.680 kb on - strand, within GFF2423 at 474.680 kb on - strand, within GFF2423 at 474.680 kb on - strand, within GFF2423 at 474.784 kb on + strand, within GFF2423 at 474.785 kb on - strand, within GFF2423 at 474.931 kb on + strand, within GFF2423 at 474.932 kb on - strand, within GFF2423 at 475.170 kb on + strand, within GFF2424 at 475.170 kb on + strand, within GFF2424 at 475.170 kb on + strand, within GFF2424 at 475.194 kb on + strand, within GFF2424 at 475.195 kb on - strand, within GFF2424 at 475.195 kb on - strand, within GFF2424 at 475.339 kb on - strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.630 kb on - strand, within GFF2424 at 475.630 kb on - strand, within GFF2424 at 475.710 kb on + strand, within GFF2424 at 475.713 kb on + strand, within GFF2424 at 475.713 kb on + strand, within GFF2424 at 475.713 kb on + strand, within GFF2424 at 475.714 kb on - strand, within GFF2424 at 475.737 kb on + strand, within GFF2424 at 475.738 kb on - strand, within GFF2424 at 475.738 kb on - strand, within GFF2424 at 475.738 kb on - strand, within GFF2424 at 475.783 kb on - strand, within GFF2424 at 475.928 kb on + strand at 475.928 kb on + strand at 475.928 kb on + strand at 475.928 kb on + strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 476.042 kb on + strand, within GFF2425 at 476.042 kb on + strand, within GFF2425 at 476.043 kb on - strand, within GFF2425 at 476.318 kb on + strand, within GFF2425 at 476.319 kb on - strand, within GFF2425 at 476.319 kb on - strand, within GFF2425
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 474,603 + GFF2423 0.44 -1.2 474,603 + GFF2423 0.44 +0.4 474,603 + GFF2423 0.44 +0.4 474,603 + GFF2423 0.44 -0.2 474,603 + GFF2423 0.44 -0.9 474,604 - GFF2423 0.44 -0.6 474,607 + GFF2423 0.44 -0.2 474,608 - GFF2423 0.45 +0.8 474,608 - GFF2423 0.45 -2.3 474,676 + GFF2423 0.53 -0.5 474,680 - GFF2423 0.54 +0.8 474,680 - GFF2423 0.54 +1.6 474,680 - GFF2423 0.54 -0.2 474,784 + GFF2423 0.67 -0.1 474,785 - GFF2423 0.67 -0.1 474,931 + GFF2423 0.85 -0.8 474,932 - GFF2423 0.85 -0.2 475,170 + GFF2424 0.15 +0.8 475,170 + GFF2424 0.15 -0.1 475,170 + GFF2424 0.15 +0.8 475,194 + GFF2424 0.18 +0.7 475,195 - GFF2424 0.18 -1.0 475,195 - GFF2424 0.18 +0.6 475,339 - GFF2424 0.35 +0.1 475,626 + GFF2424 0.68 -1.6 475,626 + GFF2424 0.68 -1.3 475,626 + GFF2424 0.68 -1.0 475,626 + GFF2424 0.68 -0.3 475,627 - GFF2424 0.69 -0.5 475,627 - GFF2424 0.69 +0.2 475,627 - GFF2424 0.69 -1.0 475,627 - GFF2424 0.69 +0.3 475,627 - GFF2424 0.69 -0.9 475,630 - GFF2424 0.69 +0.6 475,630 - GFF2424 0.69 -0.7 475,710 + GFF2424 0.78 -0.5 475,713 + GFF2424 0.79 -0.5 475,713 + GFF2424 0.79 +0.4 475,713 + GFF2424 0.79 -0.0 475,714 - GFF2424 0.79 +0.8 475,737 + GFF2424 0.81 -0.3 475,738 - GFF2424 0.82 -0.5 475,738 - GFF2424 0.82 +0.8 475,738 - GFF2424 0.82 -0.5 475,783 - GFF2424 0.87 -0.1 475,928 + +0.6 475,928 + +2.8 475,928 + -1.3 475,928 + +0.5 475,929 - +0.2 475,929 - -0.7 475,929 - -0.8 475,929 - +1.1 475,929 - +0.5 475,929 - -0.3 475,929 - -1.0 476,042 + GFF2425 0.14 -1.2 476,042 + GFF2425 0.14 +1.1 476,043 - GFF2425 0.14 -0.0 476,318 + GFF2425 0.39 -0.5 476,319 - GFF2425 0.39 +0.5 476,319 - GFF2425 0.39 -0.9
Or see this region's nucleotide sequence