Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2241 and GFF2242 overlap by 10 nucleotides GFF2242 and GFF2243 are separated by 135 nucleotides GFF2243 and GFF2244 overlap by 4 nucleotides GFF2244 and GFF2245 overlap by 4 nucleotides
GFF2241 - Transcriptional regulator, AcrR family, at 299,827 to 300,435
GFF2241
GFF2242 - no description, at 300,426 to 300,692
GFF2242
GFF2243 - Ornithine cyclodeaminase (EC 4.3.1.12), at 300,828 to 301,787
GFF2243
GFF2244 - hypothetical protein, at 301,784 to 302,647
GFF2244
GFF2245 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 302,644 to 303,219
GFF2245
Position (kb) 300
301
302 Strain fitness (log2 ratio) -2
-1
0
1 at 299.974 kb on + strand, within GFF2241 at 299.975 kb on - strand, within GFF2241 at 299.980 kb on + strand, within GFF2241 at 299.981 kb on - strand, within GFF2241 at 299.981 kb on - strand, within GFF2241 at 299.981 kb on - strand, within GFF2241 at 300.196 kb on + strand, within GFF2241 at 300.196 kb on + strand, within GFF2241 at 300.196 kb on + strand, within GFF2241 at 300.197 kb on - strand, within GFF2241 at 300.197 kb on - strand, within GFF2241 at 300.197 kb on - strand, within GFF2241 at 300.397 kb on + strand at 300.397 kb on + strand at 300.398 kb on - strand at 300.398 kb on - strand at 300.433 kb on + strand at 300.434 kb on - strand at 300.520 kb on + strand, within GFF2242 at 300.529 kb on + strand, within GFF2242 at 300.529 kb on + strand, within GFF2242 at 300.529 kb on + strand, within GFF2242 at 300.529 kb on + strand, within GFF2242 at 300.529 kb on + strand, within GFF2242 at 300.529 kb on + strand, within GFF2242 at 300.529 kb on + strand, within GFF2242 at 300.530 kb on - strand, within GFF2242 at 300.530 kb on - strand, within GFF2242 at 300.530 kb on - strand, within GFF2242 at 300.530 kb on - strand, within GFF2242 at 300.530 kb on - strand, within GFF2242 at 300.766 kb on + strand at 300.766 kb on + strand at 300.767 kb on - strand at 300.777 kb on - strand at 301.522 kb on + strand, within GFF2243 at 301.522 kb on + strand, within GFF2243 at 301.522 kb on + strand, within GFF2243 at 301.523 kb on - strand, within GFF2243 at 301.523 kb on - strand, within GFF2243 at 301.523 kb on - strand, within GFF2243 at 302.017 kb on - strand, within GFF2244 at 302.017 kb on - strand, within GFF2244 at 302.095 kb on - strand, within GFF2244 at 302.095 kb on - strand, within GFF2244 at 302.233 kb on - strand, within GFF2244 at 302.328 kb on + strand, within GFF2244 at 302.328 kb on + strand, within GFF2244 at 302.328 kb on + strand, within GFF2244 at 302.328 kb on + strand, within GFF2244 at 302.329 kb on - strand, within GFF2244 at 302.329 kb on - strand, within GFF2244 at 302.554 kb on - strand, within GFF2244
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 299,974 + GFF2241 0.24 +0.3 299,975 - GFF2241 0.24 -0.2 299,980 + GFF2241 0.25 -0.0 299,981 - GFF2241 0.25 -1.7 299,981 - GFF2241 0.25 +1.3 299,981 - GFF2241 0.25 +0.4 300,196 + GFF2241 0.61 -1.3 300,196 + GFF2241 0.61 +0.1 300,196 + GFF2241 0.61 +0.0 300,197 - GFF2241 0.61 -0.6 300,197 - GFF2241 0.61 -0.5 300,197 - GFF2241 0.61 +1.1 300,397 + -2.2 300,397 + +0.3 300,398 - +1.4 300,398 - -0.7 300,433 + -0.5 300,434 - +0.4 300,520 + GFF2242 0.35 -1.3 300,529 + GFF2242 0.39 -0.3 300,529 + GFF2242 0.39 +0.6 300,529 + GFF2242 0.39 +0.0 300,529 + GFF2242 0.39 -1.6 300,529 + GFF2242 0.39 +0.8 300,529 + GFF2242 0.39 -0.1 300,529 + GFF2242 0.39 -1.6 300,530 - GFF2242 0.39 -0.8 300,530 - GFF2242 0.39 +1.0 300,530 - GFF2242 0.39 +0.0 300,530 - GFF2242 0.39 +0.0 300,530 - GFF2242 0.39 +1.7 300,766 + -1.1 300,766 + -2.2 300,767 - +0.9 300,777 - -0.1 301,522 + GFF2243 0.72 -0.6 301,522 + GFF2243 0.72 -1.8 301,522 + GFF2243 0.72 -0.5 301,523 - GFF2243 0.72 -0.0 301,523 - GFF2243 0.72 +0.5 301,523 - GFF2243 0.72 -0.1 302,017 - GFF2244 0.27 -0.6 302,017 - GFF2244 0.27 -0.8 302,095 - GFF2244 0.36 -0.2 302,095 - GFF2244 0.36 +0.3 302,233 - GFF2244 0.52 +0.5 302,328 + GFF2244 0.63 -1.5 302,328 + GFF2244 0.63 -2.3 302,328 + GFF2244 0.63 -0.5 302,328 + GFF2244 0.63 +0.7 302,329 - GFF2244 0.63 +1.3 302,329 - GFF2244 0.63 -1.1 302,554 - GFF2244 0.89 -0.2
Or see this region's nucleotide sequence