Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2084 and GFF2085 overlap by 4 nucleotides GFF2085 and GFF2086 overlap by 4 nucleotides GFF2086 and GFF2087 overlap by 11 nucleotides
GFF2084 - Phosphatidate cytidylyltransferase (EC 2.7.7.41), at 131,960 to 132,913
GFF2084
GFF2085 - Acyl-CoA:1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 132,910 to 133,638
GFF2085
GFF2086 - hypothetical protein, at 133,635 to 134,084
GFF2086
GFF2087 - Ser/Thr and Tyr protein phosphatase (dual specificity), at 134,074 to 135,423
GFF2087
Position (kb) 133
134
135 Strain fitness (log2 ratio) -1
0
1
2 at 132.639 kb on + strand, within GFF2084 at 132.640 kb on - strand, within GFF2084 at 132.670 kb on - strand, within GFF2084 at 132.670 kb on - strand, within GFF2084 at 132.670 kb on - strand, within GFF2084 at 133.367 kb on + strand, within GFF2085 at 133.367 kb on + strand, within GFF2085 at 133.367 kb on + strand, within GFF2085 at 133.466 kb on + strand, within GFF2085 at 133.466 kb on + strand, within GFF2085 at 133.467 kb on - strand, within GFF2085 at 133.891 kb on + strand, within GFF2086 at 133.891 kb on + strand, within GFF2086 at 133.892 kb on - strand, within GFF2086 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.643 kb on - strand, within GFF2087 at 134.747 kb on + strand, within GFF2087 at 134.748 kb on - strand, within GFF2087 at 134.785 kb on - strand, within GFF2087 at 135.006 kb on + strand, within GFF2087 at 135.006 kb on + strand, within GFF2087 at 135.007 kb on - strand, within GFF2087
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 132,639 + GFF2084 0.71 -0.4 132,640 - GFF2084 0.71 +0.0 132,670 - GFF2084 0.74 -1.1 132,670 - GFF2084 0.74 -1.2 132,670 - GFF2084 0.74 -0.5 133,367 + GFF2085 0.63 +0.5 133,367 + GFF2085 0.63 -0.6 133,367 + GFF2085 0.63 -0.6 133,466 + GFF2085 0.76 -1.6 133,466 + GFF2085 0.76 +1.1 133,467 - GFF2085 0.76 -0.2 133,891 + GFF2086 0.57 +0.5 133,891 + GFF2086 0.57 -0.4 133,892 - GFF2086 0.57 +2.7 134,642 + GFF2087 0.42 +0.1 134,642 + GFF2087 0.42 -0.4 134,642 + GFF2087 0.42 -0.2 134,642 + GFF2087 0.42 -0.2 134,643 - GFF2087 0.42 -0.3 134,747 + GFF2087 0.50 -0.3 134,748 - GFF2087 0.50 -1.0 134,785 - GFF2087 0.53 -0.7 135,006 + GFF2087 0.69 -0.1 135,006 + GFF2087 0.69 +0.0 135,007 - GFF2087 0.69 -0.2
Or see this region's nucleotide sequence