Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2067 and GFF2068 overlap by 4 nucleotides GFF2068 and GFF2069 are separated by 152 nucleotides GFF2069 and GFF2070 overlap by 4 nucleotides GFF2070 and GFF2071 are separated by 14 nucleotides GFF2071 and GFF2072 are separated by 1 nucleotides
GFF2067 - OmpA/MotB, at 115,650 to 116,273
GFF2067
GFF2068 - hypothetical protein, at 116,270 to 116,734
GFF2068
GFF2069 - Cobalt-zinc-cadmium resistance protein, at 116,887 to 117,804
GFF2069
GFF2070 - D-aminoacyl-tRNA deacylase (EC 3.1.1.96), at 117,801 to 118,244
GFF2070
GFF2071 - Metal-dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly, at 118,259 to 118,726
GFF2071
GFF2072 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 118,728 to 119,684
GFF2072
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.776 kb on + strand at 116.776 kb on + strand at 116.776 kb on + strand at 116.777 kb on - strand at 116.777 kb on - strand at 116.777 kb on - strand at 116.780 kb on - strand at 116.793 kb on + strand at 116.793 kb on + strand at 116.794 kb on - strand at 116.794 kb on - strand at 116.794 kb on - strand at 116.794 kb on - strand at 116.797 kb on + strand at 116.797 kb on + strand at 116.797 kb on + strand at 116.797 kb on + strand at 116.797 kb on + strand at 116.798 kb on - strand at 116.798 kb on - strand at 116.798 kb on - strand at 116.798 kb on - strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.840 kb on + strand at 116.841 kb on - strand at 116.967 kb on + strand at 116.968 kb on + strand at 116.968 kb on + strand at 116.968 kb on + strand at 116.968 kb on + strand at 116.968 kb on + strand at 116.969 kb on - strand at 116.969 kb on - strand at 116.969 kb on - strand at 116.969 kb on - strand at 116.969 kb on - strand at 116.969 kb on - strand at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.113 kb on - strand, within GFF2069 at 117.113 kb on - strand, within GFF2069 at 117.113 kb on - strand, within GFF2069 at 117.801 kb on - strand at 117.811 kb on + strand at 117.811 kb on + strand at 117.811 kb on + strand at 117.811 kb on + strand at 117.811 kb on + strand at 117.812 kb on - strand at 117.812 kb on - strand at 117.812 kb on - strand at 117.812 kb on - strand at 117.842 kb on + strand at 117.842 kb on + strand at 117.842 kb on + strand at 117.843 kb on - strand at 117.843 kb on - strand at 118.116 kb on + strand, within GFF2070 at 118.116 kb on + strand, within GFF2070 at 118.117 kb on - strand, within GFF2070 at 118.122 kb on + strand, within GFF2070 at 118.122 kb on + strand, within GFF2070 at 118.122 kb on + strand, within GFF2070 at 118.122 kb on + strand, within GFF2070 at 118.122 kb on + strand, within GFF2070 at 118.122 kb on + strand, within GFF2070 at 118.123 kb on - strand, within GFF2070 at 118.123 kb on - strand, within GFF2070 at 118.123 kb on - strand, within GFF2070 at 118.123 kb on - strand, within GFF2070 at 118.123 kb on - strand, within GFF2070 at 118.123 kb on - strand, within GFF2070 at 118.242 kb on + strand at 118.242 kb on + strand at 118.242 kb on + strand at 118.243 kb on - strand at 118.243 kb on - strand at 118.762 kb on + strand at 118.762 kb on + strand at 118.762 kb on + strand at 118.762 kb on + strand at 118.762 kb on + strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 115,960 + GFF2067 0.50 +0.2 115,960 + GFF2067 0.50 +0.5 115,960 + GFF2067 0.50 +0.9 115,960 + GFF2067 0.50 +0.3 115,960 + GFF2067 0.50 +0.3 115,961 - GFF2067 0.50 -0.0 115,961 - GFF2067 0.50 -0.3 115,961 - GFF2067 0.50 -1.2 115,961 - GFF2067 0.50 -0.3 115,961 - GFF2067 0.50 -1.1 115,961 - GFF2067 0.50 -0.3 115,961 - GFF2067 0.50 -0.8 115,961 - GFF2067 0.50 -0.3 115,961 - GFF2067 0.50 -0.1 115,961 - GFF2067 0.50 +1.3 115,961 - GFF2067 0.50 -0.5 115,961 - GFF2067 0.50 -1.0 115,961 - GFF2067 0.50 +0.3 115,969 + GFF2067 0.51 -0.4 115,969 + GFF2067 0.51 -0.1 115,969 + GFF2067 0.51 +0.4 115,969 + GFF2067 0.51 -0.7 115,970 - GFF2067 0.51 +2.3 115,970 - GFF2067 0.51 -0.1 115,970 - GFF2067 0.51 -1.1 115,970 - GFF2067 0.51 -0.4 116,093 - GFF2067 0.71 +0.2 116,093 - GFF2067 0.71 +1.6 116,093 - GFF2067 0.71 +0.2 116,093 - GFF2067 0.71 -0.8 116,776 + -1.5 116,776 + -0.3 116,776 + +0.4 116,777 - -1.1 116,777 - -1.2 116,777 - -0.6 116,780 - -0.3 116,793 + -2.7 116,793 + -0.1 116,794 - +0.0 116,794 - -0.2 116,794 - +0.0 116,794 - -2.8 116,797 + -1.9 116,797 + -0.1 116,797 + -0.4 116,797 + -0.4 116,797 + -1.8 116,798 - +0.3 116,798 - -0.4 116,798 - -0.3 116,798 - +0.2 116,837 + -0.6 116,837 + -0.1 116,837 + +0.1 116,837 + -2.1 116,837 + -0.5 116,837 + +0.3 116,837 + -0.9 116,837 + -0.4 116,837 + +0.6 116,837 + -2.0 116,837 + +0.9 116,837 + +0.3 116,837 + +0.2 116,838 - +0.5 116,838 - -0.4 116,838 - +1.2 116,838 - +0.1 116,838 - -0.2 116,838 - -0.2 116,838 - -1.0 116,838 - -1.3 116,838 - -0.0 116,838 - +0.0 116,838 - +0.4 116,840 + +0.1 116,841 - +0.1 116,967 + +0.5 116,968 + -0.5 116,968 + -1.2 116,968 + -0.2 116,968 + +0.9 116,968 + +0.1 116,969 - +0.6 116,969 - -0.1 116,969 - -0.4 116,969 - +0.0 116,969 - +0.5 116,969 - -1.4 117,112 + GFF2069 0.25 +1.1 117,112 + GFF2069 0.25 -0.0 117,112 + GFF2069 0.25 +0.3 117,112 + GFF2069 0.25 +0.8 117,112 + GFF2069 0.25 -0.5 117,112 + GFF2069 0.25 +0.5 117,112 + GFF2069 0.25 -0.3 117,112 + GFF2069 0.25 -1.7 117,112 + GFF2069 0.25 +1.9 117,112 + GFF2069 0.25 -0.4 117,113 - GFF2069 0.25 +0.9 117,113 - GFF2069 0.25 +0.5 117,113 - GFF2069 0.25 +0.3 117,801 - +0.0 117,811 + -0.1 117,811 + -0.3 117,811 + -1.0 117,811 + -1.1 117,811 + +1.4 117,812 - -0.2 117,812 - -1.1 117,812 - +0.0 117,812 - -0.4 117,842 + -0.6 117,842 + +0.6 117,842 + -0.9 117,843 - +0.2 117,843 - -1.2 118,116 + GFF2070 0.71 -0.3 118,116 + GFF2070 0.71 +1.1 118,117 - GFF2070 0.71 -2.1 118,122 + GFF2070 0.72 +0.4 118,122 + GFF2070 0.72 +0.8 118,122 + GFF2070 0.72 -0.8 118,122 + GFF2070 0.72 +0.0 118,122 + GFF2070 0.72 +2.9 118,122 + GFF2070 0.72 -0.5 118,123 - GFF2070 0.73 -1.1 118,123 - GFF2070 0.73 -2.1 118,123 - GFF2070 0.73 -0.4 118,123 - GFF2070 0.73 -0.3 118,123 - GFF2070 0.73 +0.3 118,123 - GFF2070 0.73 -0.1 118,242 + -1.0 118,242 + +0.7 118,242 + +0.7 118,243 - -0.4 118,243 - -0.8 118,762 + +0.5 118,762 + -0.7 118,762 + +0.2 118,762 + +0.5 118,762 + +0.5 118,763 - -1.7 118,763 - -2.4 118,763 - -0.0 118,763 - -0.3 118,763 - +0.7 118,763 - -0.9 118,763 - -0.3 118,763 - -1.1
Or see this region's nucleotide sequence