Strain Fitness in Variovorax sp. SCN45 around GFF2027

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2024 and GFF2025 are separated by 15 nucleotidesGFF2025 and GFF2026 overlap by 4 nucleotidesGFF2026 and GFF2027 overlap by 4 nucleotidesGFF2027 and GFF2028 are separated by 181 nucleotides GFF2024 - L-lysine permease, at 68,154 to 68,795 GFF2024 GFF2025 - Uncharacterized protein SCO3165, at 68,811 to 69,299 GFF2025 GFF2026 - Predicted Fe-S protein, at 69,296 to 69,553 GFF2026 GFF2027 - 2-hydroxychromene-2-carboxylate isomerase family protein, at 69,550 to 70,197 GFF2027 GFF2028 - Uncharacterized MFS-type transporter, at 70,379 to 72,073 GFF2028 Position (kb) 69 70 71Strain fitness (log2 ratio) -2 -1 0 1 2 3at 68.664 kb on + strand, within GFF2024at 68.664 kb on + strand, within GFF2024at 68.796 kb on + strandat 68.796 kb on + strandat 68.796 kb on + strandat 68.797 kb on - strandat 68.829 kb on - strandat 69.023 kb on + strand, within GFF2025at 69.023 kb on + strand, within GFF2025at 69.023 kb on + strand, within GFF2025at 69.023 kb on + strand, within GFF2025at 69.024 kb on - strand, within GFF2025at 69.024 kb on - strand, within GFF2025at 69.024 kb on - strand, within GFF2025at 69.024 kb on - strand, within GFF2025at 69.024 kb on - strand, within GFF2025at 69.590 kb on - strandat 69.640 kb on + strand, within GFF2027at 69.640 kb on + strand, within GFF2027at 69.640 kb on + strand, within GFF2027at 69.640 kb on + strand, within GFF2027at 69.640 kb on + strand, within GFF2027at 69.640 kb on + strand, within GFF2027at 69.641 kb on - strand, within GFF2027at 69.641 kb on - strand, within GFF2027at 69.641 kb on - strand, within GFF2027at 69.641 kb on - strand, within GFF2027at 69.641 kb on - strand, within GFF2027at 69.652 kb on + strand, within GFF2027at 69.652 kb on + strand, within GFF2027at 69.653 kb on - strand, within GFF2027at 69.653 kb on - strand, within GFF2027at 69.653 kb on - strand, within GFF2027at 69.736 kb on + strand, within GFF2027at 69.736 kb on + strand, within GFF2027at 69.736 kb on + strand, within GFF2027at 69.737 kb on - strand, within GFF2027at 69.737 kb on - strand, within GFF2027at 69.737 kb on - strand, within GFF2027at 69.802 kb on + strand, within GFF2027at 69.802 kb on + strand, within GFF2027at 69.802 kb on + strand, within GFF2027at 69.802 kb on + strand, within GFF2027at 69.802 kb on + strand, within GFF2027at 69.803 kb on - strand, within GFF2027at 69.803 kb on - strand, within GFF2027at 69.803 kb on - strand, within GFF2027at 69.803 kb on - strand, within GFF2027at 70.051 kb on + strand, within GFF2027at 70.051 kb on + strand, within GFF2027at 70.051 kb on + strand, within GFF2027at 70.051 kb on + strand, within GFF2027at 70.051 kb on + strand, within GFF2027at 70.051 kb on + strand, within GFF2027at 70.051 kb on + strand, within GFF2027at 70.051 kb on + strand, within GFF2027at 70.051 kb on + strand, within GFF2027at 70.052 kb on - strand, within GFF2027at 70.052 kb on - strand, within GFF2027at 70.052 kb on - strand, within GFF2027at 70.052 kb on - strand, within GFF2027at 70.052 kb on - strand, within GFF2027at 70.052 kb on - strand, within GFF2027at 70.108 kb on + strand, within GFF2027at 70.108 kb on + strand, within GFF2027at 70.108 kb on - strand, within GFF2027at 70.109 kb on - strand, within GFF2027at 70.260 kb on + strandat 70.261 kb on - strandat 70.261 kb on - strandat 70.267 kb on + strandat 70.360 kb on + strandat 70.360 kb on + strandat 70.360 kb on + strandat 70.360 kb on + strandat 70.360 kb on + strandat 70.360 kb on + strandat 70.360 kb on + strandat 70.360 kb on + strandat 70.360 kb on + strandat 70.360 kb on + strandat 70.361 kb on - strandat 70.361 kb on - strandat 70.361 kb on - strandat 70.361 kb on - strandat 70.361 kb on - strandat 70.361 kb on - strandat 70.361 kb on - strandat 70.392 kb on + strandat 70.392 kb on + strandat 70.392 kb on + strandat 70.392 kb on + strandat 70.393 kb on - strandat 70.393 kb on - strandat 70.393 kb on - strandat 70.491 kb on - strandat 70.499 kb on + strandat 70.499 kb on + strandat 70.499 kb on + strandat 70.499 kb on + strandat 70.500 kb on - strandat 70.500 kb on - strandat 70.500 kb on - strandat 70.500 kb on - strandat 70.500 kb on - strandat 70.505 kb on + strandat 70.664 kb on + strand, within GFF2028at 70.664 kb on + strand, within GFF2028at 70.664 kb on + strand, within GFF2028at 70.664 kb on + strand, within GFF2028at 70.664 kb on + strand, within GFF2028at 70.664 kb on + strand, within GFF2028at 70.664 kb on + strand, within GFF2028at 70.664 kb on + strand, within GFF2028at 70.665 kb on - strand, within GFF2028at 70.665 kb on - strand, within GFF2028at 70.665 kb on - strand, within GFF2028at 70.665 kb on - strand, within GFF2028at 70.665 kb on - strand, within GFF2028at 70.665 kb on - strand, within GFF2028at 70.665 kb on - strand, within GFF2028at 70.665 kb on - strand, within GFF2028at 70.808 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.826 kb on + strand, within GFF2028at 70.827 kb on - strand, within GFF2028at 70.827 kb on - strand, within GFF2028at 70.827 kb on - strand, within GFF2028at 70.827 kb on - strand, within GFF2028at 70.827 kb on - strand, within GFF2028at 70.827 kb on - strand, within GFF2028at 70.827 kb on - strand, within GFF2028at 70.827 kb on - strand, within GFF2028at 70.865 kb on + strand, within GFF2028at 70.865 kb on + strand, within GFF2028at 70.865 kb on + strand, within GFF2028at 70.865 kb on + strand, within GFF2028at 70.865 kb on + strand, within GFF2028at 70.866 kb on - strand, within GFF2028at 70.866 kb on - strand, within GFF2028at 70.866 kb on - strand, within GFF2028at 71.183 kb on + strand, within GFF2028at 71.184 kb on - strand, within GFF2028at 71.184 kb on - strand, within GFF2028at 71.184 kb on - strand, within GFF2028

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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68,664 + GFF2024 0.79 -0.3
68,664 + GFF2024 0.79 +0.2
68,796 + +2.1
68,796 + -0.8
68,796 + -0.8
68,797 - +0.0
68,829 - -1.2
69,023 + GFF2025 0.43 -0.3
69,023 + GFF2025 0.43 -1.5
69,023 + GFF2025 0.43 -0.8
69,023 + GFF2025 0.43 -0.8
69,024 - GFF2025 0.44 +0.6
69,024 - GFF2025 0.44 +1.3
69,024 - GFF2025 0.44 -1.7
69,024 - GFF2025 0.44 -0.2
69,024 - GFF2025 0.44 -0.7
69,590 - -1.0
69,640 + GFF2027 0.14 -0.9
69,640 + GFF2027 0.14 -0.6
69,640 + GFF2027 0.14 -1.0
69,640 + GFF2027 0.14 -0.7
69,640 + GFF2027 0.14 -0.7
69,640 + GFF2027 0.14 -1.8
69,641 - GFF2027 0.14 -0.8
69,641 - GFF2027 0.14 -1.8
69,641 - GFF2027 0.14 -1.1
69,641 - GFF2027 0.14 +0.3
69,641 - GFF2027 0.14 +0.3
69,652 + GFF2027 0.16 -0.6
69,652 + GFF2027 0.16 -0.4
69,653 - GFF2027 0.16 -0.5
69,653 - GFF2027 0.16 -1.3
69,653 - GFF2027 0.16 -1.4
69,736 + GFF2027 0.29 +0.7
69,736 + GFF2027 0.29 -2.0
69,736 + GFF2027 0.29 -0.4
69,737 - GFF2027 0.29 -0.3
69,737 - GFF2027 0.29 +0.3
69,737 - GFF2027 0.29 -1.6
69,802 + GFF2027 0.39 -0.4
69,802 + GFF2027 0.39 -0.9
69,802 + GFF2027 0.39 -0.7
69,802 + GFF2027 0.39 -2.0
69,802 + GFF2027 0.39 +0.2
69,803 - GFF2027 0.39 -0.0
69,803 - GFF2027 0.39 -0.0
69,803 - GFF2027 0.39 +0.6
69,803 - GFF2027 0.39 -2.2
70,051 + GFF2027 0.77 -0.5
70,051 + GFF2027 0.77 -0.3
70,051 + GFF2027 0.77 -0.7
70,051 + GFF2027 0.77 -2.0
70,051 + GFF2027 0.77 -0.3
70,051 + GFF2027 0.77 -1.7
70,051 + GFF2027 0.77 -2.5
70,051 + GFF2027 0.77 -1.1
70,051 + GFF2027 0.77 -0.4
70,052 - GFF2027 0.77 -0.1
70,052 - GFF2027 0.77 -1.5
70,052 - GFF2027 0.77 -2.0
70,052 - GFF2027 0.77 +0.3
70,052 - GFF2027 0.77 -0.9
70,052 - GFF2027 0.77 -1.0
70,108 + GFF2027 0.86 -1.5
70,108 + GFF2027 0.86 -0.7
70,108 - GFF2027 0.86 -0.7
70,109 - GFF2027 0.86 +0.1
70,260 + +0.1
70,261 - -0.8
70,261 - -0.7
70,267 + +0.1
70,360 + -0.2
70,360 + -2.0
70,360 + -0.7
70,360 + -0.2
70,360 + +0.6
70,360 + -1.6
70,360 + +0.3
70,360 + +0.3
70,360 + -0.3
70,360 + -0.1
70,361 - -1.0
70,361 - -0.2
70,361 - -0.2
70,361 - -0.4
70,361 - -1.0
70,361 - +0.6
70,361 - +3.4
70,392 + -1.6
70,392 + -0.5
70,392 + +0.1
70,392 + -0.8
70,393 - -0.5
70,393 - -1.8
70,393 - +2.3
70,491 - +0.3
70,499 + -0.6
70,499 + +0.1
70,499 + -0.2
70,499 + +1.4
70,500 - +0.1
70,500 - -1.1
70,500 - -1.3
70,500 - +0.7
70,500 - -0.7
70,505 + -0.5
70,664 + GFF2028 0.17 +0.3
70,664 + GFF2028 0.17 -0.6
70,664 + GFF2028 0.17 +0.3
70,664 + GFF2028 0.17 +0.1
70,664 + GFF2028 0.17 -0.1
70,664 + GFF2028 0.17 +0.4
70,664 + GFF2028 0.17 -0.9
70,664 + GFF2028 0.17 +0.5
70,665 - GFF2028 0.17 -0.1
70,665 - GFF2028 0.17 -2.4
70,665 - GFF2028 0.17 -1.7
70,665 - GFF2028 0.17 -0.6
70,665 - GFF2028 0.17 -0.1
70,665 - GFF2028 0.17 -0.3
70,665 - GFF2028 0.17 -0.4
70,665 - GFF2028 0.17 -1.0
70,808 + GFF2028 0.25 +0.3
70,826 + GFF2028 0.26 -0.5
70,826 + GFF2028 0.26 +0.1
70,826 + GFF2028 0.26 +1.7
70,826 + GFF2028 0.26 -0.5
70,826 + GFF2028 0.26 -0.9
70,826 + GFF2028 0.26 -0.7
70,826 + GFF2028 0.26 -1.1
70,826 + GFF2028 0.26 -1.1
70,826 + GFF2028 0.26 -0.3
70,826 + GFF2028 0.26 -0.8
70,826 + GFF2028 0.26 -1.0
70,826 + GFF2028 0.26 -0.6
70,826 + GFF2028 0.26 -0.6
70,826 + GFF2028 0.26 -0.1
70,826 + GFF2028 0.26 -0.6
70,827 - GFF2028 0.26 -0.5
70,827 - GFF2028 0.26 +0.2
70,827 - GFF2028 0.26 +0.1
70,827 - GFF2028 0.26 -0.4
70,827 - GFF2028 0.26 +0.3
70,827 - GFF2028 0.26 +0.4
70,827 - GFF2028 0.26 -0.4
70,827 - GFF2028 0.26 -1.4
70,865 + GFF2028 0.29 -0.4
70,865 + GFF2028 0.29 -0.3
70,865 + GFF2028 0.29 +0.3
70,865 + GFF2028 0.29 +0.6
70,865 + GFF2028 0.29 +1.6
70,866 - GFF2028 0.29 +0.1
70,866 - GFF2028 0.29 -0.2
70,866 - GFF2028 0.29 -1.8
71,183 + GFF2028 0.47 +0.1
71,184 - GFF2028 0.47 -0.4
71,184 - GFF2028 0.47 -0.5
71,184 - GFF2028 0.47 +0.3

Or see this region's nucleotide sequence