Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1784 and GFF1785 are separated by 112 nucleotides GFF1785 and GFF1786 overlap by 4 nucleotides GFF1786 and GFF1787 are separated by 8 nucleotides
GFF1784 - no description, at 445,163 to 445,852
GFF1784
GFF1785 - Two-component transcriptional response regulator, OmpR family, at 445,965 to 446,654
GFF1785
GFF1786 - hypothetical protein, at 446,651 to 448,003
GFF1786
GFF1787 - no description, at 448,012 to 448,167
GFF1787
Position (kb) 446
447
448
449 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 445.716 kb on + strand, within GFF1784 at 445.717 kb on - strand, within GFF1784 at 445.803 kb on + strand at 445.804 kb on - strand at 445.867 kb on + strand at 445.868 kb on - strand at 445.868 kb on - strand at 445.868 kb on - strand at 445.868 kb on - strand at 445.917 kb on + strand at 446.256 kb on + strand, within GFF1785 at 446.617 kb on - strand at 446.617 kb on - strand at 447.374 kb on + strand, within GFF1786 at 447.374 kb on + strand, within GFF1786 at 447.374 kb on + strand, within GFF1786 at 447.375 kb on - strand, within GFF1786 at 447.375 kb on - strand, within GFF1786 at 447.819 kb on + strand, within GFF1786 at 448.165 kb on + strand at 448.165 kb on + strand at 448.166 kb on - strand at 448.174 kb on + strand at 448.174 kb on + strand at 448.175 kb on - strand at 448.175 kb on - strand at 448.371 kb on - strand at 448.474 kb on + strand at 448.612 kb on + strand at 448.612 kb on + strand at 448.612 kb on + strand at 448.613 kb on - strand at 448.844 kb on - strand at 448.942 kb on + strand at 448.946 kb on - strand at 448.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 445,716 + GFF1784 0.80 +0.3 445,717 - GFF1784 0.80 -0.2 445,803 + -2.0 445,804 - +0.3 445,867 + -0.2 445,868 - +0.1 445,868 - -2.0 445,868 - +1.2 445,868 - +1.2 445,917 + +0.6 446,256 + GFF1785 0.42 +1.0 446,617 - -1.3 446,617 - -0.7 447,374 + GFF1786 0.53 -1.6 447,374 + GFF1786 0.53 +0.4 447,374 + GFF1786 0.53 -3.0 447,375 - GFF1786 0.54 -0.1 447,375 - GFF1786 0.54 -0.4 447,819 + GFF1786 0.86 -1.7 448,165 + -0.5 448,165 + +0.3 448,166 - +0.5 448,174 + -0.2 448,174 + -0.2 448,175 - +0.6 448,175 - -1.0 448,371 - -1.3 448,474 + -1.3 448,612 + +0.7 448,612 + +0.1 448,612 + -0.4 448,613 - -0.0 448,844 - -0.3 448,942 + +1.0 448,946 - +0.1 448,946 - +0.2
Or see this region's nucleotide sequence