Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1773 and GFF1774 are separated by 128 nucleotides GFF1774 and GFF1775 overlap by 4 nucleotides GFF1775 and GFF1776 are separated by 28 nucleotides GFF1776 and GFF1777 are separated by 24 nucleotides
GFF1773 - D-amino acid dehydrogenase (EC 1.4.99.6), at 435,330 to 436,631
GFF1773
GFF1774 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 436,760 to 437,227
GFF1774
GFF1775 - no description, at 437,224 to 437,718
GFF1775
GFF1776 - OsmC-like protein Pfl_1628, at 437,747 to 438,148
GFF1776
GFF1777 - Beta-lactamase class C-like and penicillin binding proteins (PBPs) superfamily, at 438,173 to 439,354
GFF1777
Position (kb) 436
437
438 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 435.817 kb on + strand, within GFF1773 at 435.818 kb on - strand, within GFF1773 at 435.862 kb on + strand, within GFF1773 at 435.862 kb on + strand, within GFF1773 at 435.863 kb on - strand, within GFF1773 at 435.883 kb on + strand, within GFF1773 at 435.883 kb on + strand, within GFF1773 at 435.884 kb on - strand, within GFF1773 at 436.141 kb on + strand, within GFF1773 at 436.141 kb on + strand, within GFF1773 at 436.141 kb on + strand, within GFF1773 at 436.142 kb on - strand, within GFF1773 at 436.164 kb on + strand, within GFF1773 at 436.243 kb on + strand, within GFF1773 at 436.243 kb on + strand, within GFF1773 at 436.243 kb on + strand, within GFF1773 at 436.244 kb on - strand, within GFF1773 at 436.244 kb on - strand, within GFF1773 at 436.291 kb on + strand, within GFF1773 at 436.292 kb on - strand, within GFF1773 at 436.292 kb on - strand, within GFF1773 at 436.330 kb on + strand, within GFF1773 at 436.330 kb on + strand, within GFF1773 at 436.331 kb on - strand, within GFF1773 at 436.480 kb on + strand, within GFF1773 at 436.579 kb on + strand at 436.579 kb on + strand at 436.579 kb on + strand at 436.579 kb on + strand at 436.579 kb on + strand at 436.579 kb on + strand at 436.579 kb on + strand at 436.579 kb on + strand at 436.579 kb on + strand at 436.579 kb on + strand at 436.580 kb on - strand at 436.580 kb on - strand at 436.580 kb on - strand at 436.580 kb on - strand at 436.580 kb on - strand at 436.580 kb on - strand at 436.580 kb on - strand at 436.580 kb on - strand at 436.580 kb on - strand at 436.582 kb on + strand at 436.582 kb on + strand at 436.582 kb on + strand at 436.582 kb on + strand at 436.582 kb on + strand at 436.583 kb on - strand at 436.583 kb on - strand at 436.583 kb on - strand at 436.668 kb on + strand at 436.669 kb on - strand at 436.819 kb on + strand, within GFF1774 at 436.819 kb on + strand, within GFF1774 at 437.078 kb on + strand, within GFF1774 at 437.079 kb on - strand, within GFF1774 at 437.079 kb on - strand, within GFF1774 at 437.174 kb on + strand, within GFF1774 at 437.174 kb on + strand, within GFF1774 at 437.174 kb on + strand, within GFF1774 at 437.174 kb on + strand, within GFF1774 at 437.174 kb on + strand, within GFF1774 at 437.174 kb on + strand, within GFF1774 at 437.174 kb on + strand, within GFF1774 at 437.174 kb on + strand, within GFF1774 at 437.175 kb on - strand, within GFF1774 at 437.175 kb on - strand, within GFF1774 at 437.175 kb on - strand, within GFF1774 at 437.175 kb on - strand, within GFF1774 at 437.175 kb on - strand, within GFF1774 at 437.175 kb on - strand, within GFF1774 at 437.175 kb on - strand, within GFF1774 at 437.390 kb on - strand, within GFF1775 at 437.621 kb on - strand, within GFF1775 at 437.621 kb on - strand, within GFF1775 at 437.695 kb on + strand at 437.695 kb on + strand at 437.696 kb on - strand at 437.696 kb on - strand at 437.879 kb on + strand, within GFF1776 at 437.880 kb on - strand, within GFF1776 at 437.924 kb on + strand, within GFF1776 at 437.924 kb on + strand, within GFF1776 at 437.925 kb on - strand, within GFF1776 at 437.925 kb on - strand, within GFF1776
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 435,817 + GFF1773 0.37 -0.5 435,818 - GFF1773 0.37 -0.1 435,862 + GFF1773 0.41 +0.1 435,862 + GFF1773 0.41 +0.1 435,863 - GFF1773 0.41 +0.1 435,883 + GFF1773 0.42 +0.8 435,883 + GFF1773 0.42 +0.0 435,884 - GFF1773 0.43 +0.5 436,141 + GFF1773 0.62 +0.5 436,141 + GFF1773 0.62 -1.5 436,141 + GFF1773 0.62 -0.6 436,142 - GFF1773 0.62 +1.5 436,164 + GFF1773 0.64 -1.0 436,243 + GFF1773 0.70 -0.9 436,243 + GFF1773 0.70 -0.4 436,243 + GFF1773 0.70 -0.3 436,244 - GFF1773 0.70 -0.8 436,244 - GFF1773 0.70 -1.0 436,291 + GFF1773 0.74 -0.2 436,292 - GFF1773 0.74 -1.0 436,292 - GFF1773 0.74 +1.2 436,330 + GFF1773 0.77 +0.1 436,330 + GFF1773 0.77 +0.7 436,331 - GFF1773 0.77 -0.0 436,480 + GFF1773 0.88 -1.0 436,579 + +1.8 436,579 + +0.4 436,579 + -0.2 436,579 + +0.5 436,579 + -1.1 436,579 + -0.3 436,579 + -1.7 436,579 + +0.4 436,579 + +0.1 436,579 + +0.9 436,580 - +0.4 436,580 - -0.8 436,580 - -0.5 436,580 - -0.7 436,580 - +0.4 436,580 - +0.3 436,580 - -0.7 436,580 - +0.3 436,580 - -0.2 436,582 + -0.9 436,582 + -0.9 436,582 + -0.8 436,582 + +0.9 436,582 + +0.5 436,583 - -1.0 436,583 - -0.5 436,583 - -0.8 436,668 + -1.8 436,669 - -0.5 436,819 + GFF1774 0.13 -0.7 436,819 + GFF1774 0.13 -0.9 437,078 + GFF1774 0.68 -0.3 437,079 - GFF1774 0.68 -0.9 437,079 - GFF1774 0.68 -0.2 437,174 + GFF1774 0.88 -1.0 437,174 + GFF1774 0.88 -0.9 437,174 + GFF1774 0.88 +0.2 437,174 + GFF1774 0.88 -2.5 437,174 + GFF1774 0.88 -0.5 437,174 + GFF1774 0.88 -1.5 437,174 + GFF1774 0.88 -0.7 437,174 + GFF1774 0.88 +0.7 437,175 - GFF1774 0.89 -0.0 437,175 - GFF1774 0.89 -0.7 437,175 - GFF1774 0.89 -1.0 437,175 - GFF1774 0.89 -0.7 437,175 - GFF1774 0.89 -1.3 437,175 - GFF1774 0.89 -0.9 437,175 - GFF1774 0.89 -3.6 437,390 - GFF1775 0.34 -1.5 437,621 - GFF1775 0.80 -0.5 437,621 - GFF1775 0.80 +1.1 437,695 + -0.3 437,695 + -1.0 437,696 - -1.7 437,696 - +0.4 437,879 + GFF1776 0.33 -0.7 437,880 - GFF1776 0.33 +0.8 437,924 + GFF1776 0.44 -0.7 437,924 + GFF1776 0.44 +0.5 437,925 - GFF1776 0.44 -0.8 437,925 - GFF1776 0.44 +0.4
Or see this region's nucleotide sequence